Académique Documents
Professionnel Documents
Culture Documents
. .
2009 2010
microbiology
:
Micro organisms ,
%50 %90
( yelliw .)2008
Biosphere
.
.
.
.
microbiology
( )
()
microbiology
Viroids
Prions
Viruses
Eucarya
Archaea
Bacteria
( )
Helminths
Fungi
Protozoa
Algae
Protest
( :)1
: :
The discovery of microorganisms and the conflict over spontaneous generation
,
() . (- 980
)1037
Roger Bacon
microbiology
sse alinciz
)1658( Kircher
. Antony Van
)1723 -1632( Leeuwenhoek ,
( 300-40)
1676
.
,
( )322-384 Aristotale "
, "
Spontaneous generation ,
, Pasteur 1861
,
. Chon Spores
Tyndall 1872
Tyndallization .
(" )2"a,b,c 1456
. 2005
microbiology
( 60-55) 63
.Pasteurization (-1877
)1885 Anthrax
.
Wendell Holmes ( )1843
.
Lister 1878 ,
.
)1910-1843( Robert Koch
Bacillus anthracis
( )1881
. 1882 Vibrio
Cholerae 1883
( ).
)1823-1749( Jenner 1796
.
. 1880
Vaccination .
Rabies
. Roux
Antitoxins Metchinkoff
. Klebs&Loeffler
.
Rickettisa 1909
( . )2
: :
The Development of industrial Microbiology and Microbial Ecology :
. Burrill
microbiology
,1883
smith1900
schloesing&muntz 1877
nitrification , winogradsky 1890
.
Waksman
Alexanders .Dommergues Hansen 1879
.
, Weizmann .
Microbial Ecology
( (1953 -1856 (1931-1851( Beijerink
.
.
,
( . .)2
1958 Tatum , Bradme , Lederberg
. 1969 Luria , Hershey , Delbruck
Bacteriophages 1974 Claude
. 1975
Baltimore , Temin , Dulbruck
DNA . RNA
1977 Woesse ,
.1979
Scanning microscopes .1980
Hepatitis B , B ,1982
1995
. 1986
.1996 Colerae ( Chromosomes .)2000
2002 . 2005
.HIV
SARS .2003
,
) ,)1900 - 1880 ,1945
,
microbiology
,
.
.
microbiology
.7 : Soil Microbiology
.
.8 : Industrial Microbiology
,
,
.
.9 : Microbiology of Insects
.
.11
: Space Microbiology
.
10
microbiology
,
, . ,
.
AIDA SARS
, ,
%46
.
.
.
.
1984 ) )
,
.
.
3 ,
.
.
( )
Microbial genetics ( Molecular biology
) .
.
G.beadle( 1940 )E.L.Tatum .
O.Avery C.Macleod M.McCarty DNA .
J.Ledeberg E.Tatum ,
1958 F.Crick J.Watson , DNA 1960
F.Jacob J.Monod RNAm .
11
microbiology
( )
. P.Berg
() .
.
,
...
.
,
, ,
,
1979
,
.
. 1970
.
12
microbiology
prokaryotes ,
. biomass
. ,
. () genetic,
, .
1994 .
: archaebacteria
()
peptidoglycan
)mucopeptide
( )3 :
13
microbiology
Eubacteria
:Bacteria
0,2 0,8 Vibrio 25 10
( )5
- :cocci
14
microbiology
,
(Dextran ) " ( )1
(.)5
1 : Microcci
.Microcus denitrificans
2 : Diplococci
:
.Diplococci
: Maningococi
.Neisseria
pneumococci .
3 : Streptococci
:
Steptococci .Str . faecalis
Streptodiplococci .Str.pneumomiae
-4 : Tetracocci
.
-5 :Sarcinae
.Sarcina ventriculi
-6 : Staphylococci
.Staph. Aureus
- Bacilli :Rods
(.)5
:
.Colibacilli
.Bacillus anthracis
.Bacillus diphtheria
.Bacillus fusiform
Coccobacilli
Pasteurella
.pestis
Vibrios or curved rods
( )C
.vibrio cholerae
:
15
microbiology
Bacilli .
Diplobacilli .Klebsiella pneumoniae
Streptobacilli .
, .
,
.
Sarcina bacilli .
- :spirals
, 1676
. , ,
, 15
( .)5 :
.1 Spirochaeta . S. plicatilis
.2 Cristosoira .Cristospira balbiani
.3 Treponema T.pallidum .
.4 Leptospira L. interrogans
( )5 .
) (5
16
microbiology
(
)
, .
( )6
:
-1
:Capsule
.
,
,
, .
.
-2
:cell wall
,
, ,
Mucopeptides
Acetyl glucosamine
Acetylmuramic ( Osidic ) .
( )A.Teichoic
( B -7)A
:
,
.
17
microbiology
,
( ) ,
,
.
Spheroplaste
.Lysosyme
Bacteriophages
, DNA
,
.
( )
,
, ( Lugol )
,
( )
. ,
,
, , .
:Plasma membrane
,
( %60 %40) ,
, () .Permeas
:
,
Mesosomes
( )8
( Cytochromes -Oxydases
)Cytochrome .
Mitochondrions ,
18
microbiology
,
.
,
Permease .
,
.
-1
: Cytoplasm
.70S
,
Chromatophores ,
, Poly hydroxybutyric
Volutine , .
-2
:Nucleoid
DNA
.
, . .
19
microbiology
( )p-11
.
-3
:plasmids
, DNA
,
. ,
:
( )1 : Transmissible ,
12 ,
( , 106. (40 10 ( 3-1) .
( )2 : Nontransmissible ,
( ,106.)20-3 ( 60-1) .
:
* .
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-4 Aerosomes ( Gas Vacuoles
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,
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(.)10
, ,
( ).
,
, , :
-1
:Flagella
.
, (3-
) 12 , 50 , ) (10-20
20
microbiology
-2
Fimbriae
: Pili
.pilin .
pili ( )13
20-0,2 , ( )25-3 ,
. ,
Bacteriophage
Hemagglutination ,
,Sexepilus
. pili
pilin .
21
microbiology
13 Pili E.Coli
-3
: Axial filaments
-4
: Endospoeres
,
.
( )Bacillaceae
,
,Germination
, 80 -70
, ,
Ca-dipicolinic acid
.
:
-1 .
-2 .
-3 , .
, Ca- acid
dipicolinic ,
.
, ,
, CO2
,
.
:
,
. ,
Flagella :
22
microbiology
,
.
. :
-1 :
Bacillus megaterium ( )27 , Vibrio comma
( )200 ,
( )50 , .
%0,1 ,
.
( ,
) .
-2 :
, Bacillus
.rotans
-3 : ,
,
,
, ,
, .
-4 : ,
.
-5 : ,Myxobacteria
,Cyanobacteria ,
( 5-2 ).
-6 : .
( ,)Taxis ,
( ) ,
:
... :Chemotaxis .
... :Aerotaxis ,
.
... :Phototaxis ,
.
23
microbiology
-1-
, , ,
( )
Chemo
.taxis
()
( .) DNA
Uptake .
().
, .
24
microbiology
- Actinomycetes
( Actinobacteria ) :
.
Actinomycetes Actionbacteria
Actionmycetales Actinomyces
,Actionmycetales ()Mycelium
( )Hyphae
, 0.5-1.0 ,
2( .)14
( ,)Conidia
(.)Sporangium
:
- .
- .
- ,
,
.
,Mycobacterium
Coryneform .
,
, ,
.Prokaryotea
.
,
25
microbiology
,
.
, ,
,
,
.
.
- :Cyanobacteria
, ,
( ) a-15
Heterocystes
( Ribosomes .) b-15
a Phycobiline
Phycocyanine Phycoerythrine
B-carotene Zeaxanthine ,Myxoxanthine
.
,
.
,
,Lichens ( )
.
( 50)
.
Nitrogenase
.
, Hydrogenase
Nitrogenase
:
2NH3
C2H4
26
microbiology
,
,
( )3 .
:
( )16 :
-
.
-
Phycocyanine Phycoerythirne
,
.Chromatoplasme
Volutine ,
,
.
- Nucleoplasme
. DNA
27
microbiology
Eucaryotes ()
( .)17
.
201
305 309
.
201 .
Protozoan
( )
Yeast
( : )17
.
- : Fungi
28
microbiology
.
.
.
,Heterotroph exoenzyms
.
) Saprophytes ,
) () Mycorrhiza
()
.
, Yeasts
. hyphae
( Chitin ,)18
mycelium .
, .
haustoria
( .)19
( )20 ( , ,
,)
(.)3
( : )3
()
Arbuscular mycorrhizae
( )
( )
Saccharomyces .
29
microbiology
()
.
2003 .
E.coli
.
, Genome
phanerochaete chrysosporium .
.
- :Algae
Protists ,
,Colonial Phytoplankton
Diatoms ( , )21
hydrated silica
, ( .)22
. 2003
104 / ,2
.
CO2 a
Xanthophylles
phycoerythrine , .
Golden Algae () ,Brown
(.)23
,
, (
algin Agar Carrageenan )
, , ,
.
- :Protozoa
.
,
(.)24
30
microbiology
:
Flagellates .Euglena
Ciliates
.Paramecium
Amebae
.entamoebas
( )2 ()
( )3
31
microbiology
( )2 ()
,
,
, .
, .
,
.
.
,
, .
. CO2
.
, ( ) ,
( ).
32
microbiology
( : )18 .
Ascomycetes
Rhizopu
Mucor
( : )20 .
Penicillum
Mushroom
33
microbiology
The Viruses
:
,
( )1884 .
,
, ,
( (Stanley,valens 1935
.
- :
,
,
,
, vaccinia 300-200
E.coli
(1000) ( )25 ( )
, , ,
.
Jenner 1796
,
Pasteur Rabies ,1864 Ivanoweski 1892
Beijerinck 1898 .V.M.T
Twort,
) D'Herelle ,)1918 ,
( Karrer-Duggar )1921 Stanly 1935
Best 1939 ,
,
,
.
,
Bacteriophage
Cyanophyges Mycophages
34
microbiology
,
( )26
. , ,
.
( )27
Virion .
( )28
Capsid ,Capsomeres
, ,
Envelope
. .
RNA DNA ,
.
.
()
:
.1 :
35
microbiology
,
RNA ,
, .
X 300 A 150-170 A
40 A ()29
.2 :
,
, ( )60 .
, Adenovirus
252 (.)30
.3 :
:
- Poxviruses Influenza viruses
()31
- Bacteriophages
()31
.4 :
( ,
) ,
, .
.
.
, ,
Replication Reproduction
.
Bacteriophage .
36
microbiology
,
, , ()32
.
:
.1
)Adsorption &Penetration( :
( .)33
, .
Coliphage
, Lysosyme ,
:
. . DNA , , .
37
microbiology
,
.
,
.
- ( ) Slow Viruses :
,persistence Latent ,
Reactivation
.
,
Latent .
, Slow Viral Infection Incubation
, .
,
Immunodeficit Immunosuppression
Neonatal Period ,Latency Persistency
. HIV
)Hepatitis B Viruses( B
( ) HBV ( )Herpes Viruses.
, , ,
.
DNA
Adenoviruses
Papovaviruses ,
,
1960 Herpes
38
microbiology
39
microbiology
.
27-21 1986
. C
( Nitrosamine
) ( )1986 Calum Muir A
(. )1986 L.Tomatis
, .
:
,
.
.
( , , , , ,).
DNA,RNA (
) ( ) , ,Capsomeres
, ( Replication ).
genes ,
, ,RNA
mRNA ,
.
.
- :
.1 :Viruses of Bacteria and Archaea
40
microbiology
Bacteriophages
, Protists ,
.
, ,
.
, .
,
( , , , ,)
, ,
: Lytic or Virulent phages
.Temperate or avirulent phages
(.)35
) (T
Escherichia coli ( .)7-1
DNA T3 ,T7 ,
, T3 ,T7 .
65 200 55 70 .
DNA .
T2 ,T3, T6 DNA
-5 . 5-hydroxy-mehtyl cytosine
E.Coli .T
F2 RNA
x DNA x 174
DNA .
E.Coli
FI,Fd,M13 .DNA
:
,
.
-1 : Herelle
, Shigell
41
microbiology
, pesudomonas
.
-2 :
, .
-3 : ,
. ,
( )
.
.2 () : Cyanophages
, 1963
Safferman & Morris
,
( A.MOAT .)1979
.
.
.
DNA ,
LPP-1 13-19 ,
80 . SM-1 12 , (
25 40) .
.
.
-
:
.1 :
, ,
,
.
,
42
microbiology
, , , ,
Arboviruses .
,
.
( )idae ( )imae
) (Virus ,
,Adenoviridae
(()) ,
(.)36
( )4
.2 (:)Plant Viruses
,
,
( )5 (.)37
(:)5
( )m.M
60015
4010
Tobacco mosaic
0.3
60015
40106
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0.1
4209.8
26106
Potato Virus X
0.05
26
7106
0.04
20
6106
0.01
19
3106
Tobacco ring Spot
0.2
16.5
2106
43
microbiology
Alfalfa mosaic
,
,
%60
, %40 , %30 .
, ,
, , .
,
,
. ,
,
.
. .
X .
, .
:
Mosaic Necrosis Malformation
.
,
.
- :
44
microbiology
- :
- :
. - Muskmelon vein Necrosis Virus
- :
. - Potato leaf roll virus
- :
- Onion yellow Dwarf virus
- :
Lettuce M.V - Beet M.V
45
microbiology
Basidiomycetec
Ascomycetes
Nneurospora,Peziza,Saccharomyces
18 :
Deuteromycetes
Alternaria,Penicillium,Aspergillus
:
Mucor,Plasmodiophora,Rhizopus
Phycomycetes
( )Mycophages
, .
Penicillium
chrysogenum Agaricus bisporus
Sinden ,1957 1960
.
, ,
33-41 , ( dsRNA,
.) ssRNA ( )28 Penicillium cyaneofulvum
.
, .
() ,Hyphae
.
.
.
, .
phycodnaviridae
,chlorella
.dsDNA .
46
microbiology
Protozoa
Giardia intestinalis Leishmania spp. Totiviridae
. dsRN
Mimi virus dsDNA 400
Polyphaga Acanthamoeba
amoeba
.Poxviridae Phycodnaviridae , :
- :Insect Viruses
,
Togaviridae , Flaviviridae
( ) .
, Baculoviridae, Reoviridae,
.Iridoviridae, Polydnaviridae
Ttrips Beetle Whitefly
.
. dsRNA
.dsDNA
Biological control
.
- :Pirons
47
microbiology
,
. ( )
( ) :
:
:
1730 Vison 1947 1967 1985 1990 1991 1992 Puma 1992 Scrapie
() ,
.
Stanley B. Prusiner
( )PrP
, ( )PrPc C ,
) (PrPsc sc
. ) (PrPsc
48
microbiology
, )(PrPc
() ) (PrPsc ( )
.
1997 ,
,
.
.
()7 Stanly Prusiner 1995
()7
Kuru
2600
.
1957
.
1958
Fore
49
microbiology
Creutzfeldt-Jakob
)(CJD
.
,
100
PrP..
.
80 .
--
)(GSS
50 2-6
Gerstmann .
.
.
straussler .PrP
scheinker
Insomnie fatale
,
9
.
.
.
.PrP
30 PrPc
.
)(BSE
Spongiform Encephalopathy .
Bovine
) (BSE :
1986 ( )40 ,
. 1987 ,
( )
,
,
, .
1980
50
microbiology
:
1990
( )39
, -
\ \245
51
microbiology
\ \30 , ,1996
1996\3\24 3 1996
( , ,)
.
( )1985
- 50-60.
,
27-40 .
- :
-. - . . 1990 .
.
.
.
.
52
microbiology
Microbial Nutrition
Nutrients
biosynthesis .
%70-80
:
, , , , , ,,
, .
macroelements macronutrients
53
microbiology
. (P-S-
) N-H-O-C ( )
.
.
microelement trace element
.
.
growth
factors .
.
.
.
Nutritional Types of
:Microorganisms
. ,
()I
( ) I
Autotrophs
CO2
Heterotrophs
phototrophs
Chemotrophs
54
microbiology
Lithotrophs
Organotrophs
:
- :
.1 : Autotrophs
CO2 NH3
, ()
Thiobacillus .
.2 : Heterotrophs
, ,
,
Escherichia .
.3 : Hypotrophs
, ( ,)Rickettsia
Viruses .
- :
.1 : phototrophs
Bacterial chlorophyll
CO2
:
photolithotrophs
Thiorhodaceae .Chromatium
photoorganotrophs
( )
AThiorhodaceae .Rhodopseudomonas
55
microbiology
.2 :Chemotrophs
, :
Chemolithotrophs
,SH2 , NO2 , NH3 .
Chemoorgnotrophs
, Esherichia
.
.3
:Paratrophs
, Rickettsias Chlamydias
. ( )8
, .
( )8
:
Lithotrophs
Organotrophs
56
microbiology
Photoorganotrophs
Photolithotrophs
Phototrophs
.
.
Athiorhodaceae
.Thiorhodaceae
(
.
, :
)
CO2 2 H 2O (CH 2O) H 2O O2
) (.
Chemotrophs
Chemoorgnotrophs
Chemolithotrophs
:
DH 2 A D AH 2
NH 4 3 / 2O2 NO2 H 2 O 2 H
( NH
( D DH2
.)O2
AH2 A
(.
( )41 .
.
.
.
57
microbiology
Chemotaxis
.
( E.coli )
,
, , , , (.)42
(:)42
E.coli Serine
Leucine.
()
.
:
,
,
Ribonuclease Alkaline phosphatase
.Periplasm
Amylases Clostridium
Acetobutylicum .
Myxobacteria
,
.
:
:Simple diffussion passive transport
Glycerol
(
) .
:Facilitated diffusion
()
58
microbiology
Permeases
( .)43 ( )44
().
:Active transport
, Specificity
B-galactosides permease E.Coli
,
,
.
( )42
.
: Culture Media
,
:
( )In-Vivo .
( )In-Vitro
,
Agar .
.
:
- : Enriched media
Nutrient broth ( Nutrient Agar
) .
.
- : Selective media
, .
, .
59
microbiology
, ,
.
- : Differential media
, .
,
,
.
.
- : Assay media
,
.
- : Maintenance Media
. ,
.
, ,
, .
: ()
Habitats .
,
( , ,) ( .)45
( ) .
60
microbiology
Biochemical Serological
.Pathogenicity
Microbial Growth
,
, ,
. ( ).
- :
61
microbiology
- :Binary Fission
, . ,
,
, (.)48
( 30 50 )
, 15-12
E.coli 20 Mycobacterium tuberculosis
.
- :Budding
, ,
, Yeasts
Ancalomicrobium ,Hyphomicrobium
,
( )46 ( )46
.
- : Spores
,
, Bacillus , Clostridium
,
, ( PELCZAR et al
. )1977
- : Conjugation
, ( DNA
3800 E.Coli)
.
Tatum ,Lederberg 1946 E.coli
( VB1)
, ,
.
62
microbiology
) ( F ) sexual factor (F
) ( F ) (F .
( )47 ,
) (F DNA
) ( F ) . ( F
-:
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( )log b ()48
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,
. ,
, , .
,
,
.
:
: , .
:
.
: .
-2 :Logarithmic phase
,
( ) ,
63
microbiology
, ,
. .
-3 :Stationary phase
,
, , .
( PH ).
,
.
-4 :Decline phase
, ,
, . ,
. ,
72 ,
.
- :
, ,
:
n
122 2 2 2 ..2 .
n
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Generation Time
)35-15( E.coli ,
)20-12( Mycobacterium tuberculosis , (
, ) , ,
(.)9
,
.
24 ( )
,
, Blank .
64
microbiology
, ,
, , :
-B .
-b .
-t .
-G .
-n .
,
1000
( )10 , :
b 1 2n
2n
n .
, ( )B :
()2
b B 2n
( )2 :
log b log B
log 2
()3
log 2 ( )2 0.301 :
()4
log b log B
0.301
G ()t ( : )n
()5
t
n
65
microbiology
b
) (n 3.3 log
B
()6
n ( )4:
()6
t 0.301
t
( )9 .
()
Bacteria
Protista
Fungi
-
:
66
microbiology
.1
.2
.3
-a :
.
( 0.01)
1 .
petroff hausser ( )49
.
.
-b :
(1 )
( 24 ) E.coli
. Azotobacter
( )50
( )300 30
1.
.
-c :
.
67
microbiology
.
.
.
.
( Mc CRAD )
-d :
Millipore
.
( .) 51
-e :Turbidimetrice method
.
.
.
Spectrophotometer Colorimeter ( Turbidimeter
)52 Io I
. Beer&Lambert
E : .
C .
I .
:
= -ElC
log
Transmittance ( )T T%
100 :
100
= T%
68
microbiology
absorpance ( )A
:
= - log T
A = log
.
. .
-f :
( %5.5 )
.
,
.
-g :
.
-h :
.
Nitrogenase C2H2 C2H4
.
-i :Synchronous growth
.
.
69
microbiology
Defferential centrifugation .
-j : Continuous culture
( Chemostat )53
.Turbidostat
.
:
:
-1 :
.
.
-2
.
.
.
spores
( )
.
( 15) Mycobacterium tuberculosis (
70
microbiology
Psychrophiles :
30 20 15 .
7- 30 20
( .)57
71
microbiology
Mesophiles :
40 - 25
15 10 45 .
saprophiles
37.
- Thermophiles :
60 45
45 25
75 90 .
:
Facultative thermophiles
Obligate thermophiles
( 60 )
.
.
4 PRESSURE
:
-1 :
2
650 /
2
1200 /
.parophiles
6000 -300
45 20.000
.
-2 :
72
microbiology
:
hypotonic . hypertonic . Isotonic .
Plasmolysis
.
%30.5 ( )25-5
(100 90 )
Halophilic (%25-15
) ClNa
Seratia salinaria
.
5 Oxygen concentration :
.
(:)58
- :Obligate aerobe
Azotobacter Nitrobacter
( -) .
anaerobe
:Strict
Bacteriodes Clostridium
() () .
( )Eh .
:Facultative anaerobe
vibrio Escherichia
.
73
microbiology
:Aerotolerant anaerobe
Enterococcus faecalis .
- : Microaerophile
lactobacillus
.
.
:
.1
HSCH2 COONa SODIUM THIOGLYCOLATE
.
.2
()
.
.3
+ C6H3 (OH)3 .
.4 GASPAK ( )59
.GASPAK
Palladium
.
Acidity
or
Alkalinity
-6 (:)PH
PH PH
.
PH 8-6.5
. 9 -4
74
microbiology
4 3.5
. 7 = PH
.
() Buffer Solutions
PH .
KH2PO4 K2HPO4
PH .
-7 :
2
20 72.2/
.
2
(28
/ ) ( 40/ ) Detergents
.
.
( )
.
-8 Radiation :
( :)60
Electromagnetic :
ultra violet
visible rays infra red
.
75
microbiology
Ionizing :
()
x cosmic rays .
:
- Ultra violet :
A 2950 A 2000
.
. Mutation
.
.
- ():
A1000 0.06
()
.
.
.
-9 :
Bacterio Static
Bactericidale
detergent disinfectants .....
.
.
microbistasis .
76
microbiology
.
.
-11 :
Antibiotic
.
.
:
penicillium .griseo fulvin
bacillus Bacitracinpolymyxin
Actinomyetes streptomyces
streptomycin chloramphenicol
tetracycline .erythromycin
.
. :
.i
Muramic acid
- Mucopoly saccharide -
.
Penicilinase
Staphylococcus
.
.ii
Antagonisme
( ).
77
microbiology
.iii
Permease
.Colistine & Polymyxine
.iv
.Tetracyclins
- :
Microbial Metabolism
:
. :
)Anabolism or Biosynthetic( :
...... NH3, NO3, CO2 ,
,
.Endothermic
(Catabolism or Degradative( :
, :
,
, ,
.CO2
,
, , ,
,
.
78
microbiology
, ,
.
- :Anabolism
.A :
, ,
substrate .
S
ES
E +
DNA
, ,
Adaptive Induced ,
E.coli Lactose
B-galactosides
, .
Enzyme Induction Enzyme
Repression
,
:
.a :Structural genes
mRNA ,
.mRNA
.B :Operon genes
, .mRNA
.b :Regulator genes
.
.C :ATP
79
microbiology
ATP
( .)61
61 ATB
.D :
,
Baceriochlorophyll Wassink
,1939 ) (a -CO-CH3 -CH=CH2
-C6H5 CH3 .
Chromataiaceae , Chlorobacteriaceae
, H2S
:
CO2
, ,
,Chemosynthesis
,
:
.1 :Nitrobacteria
Winogradsky
,1890 ,
:
:
,Nitrosofication
,Nitrosomsnas , :
80
microbiology
Nitratation
,Nitrobacter , :
CO2 .
.2 :Thiobacteria
, ,
,Achromatium
,Beggiatoa Thiobacillus
thioparus :
:
, :
, co2
.
.3 :Ferrobacteria
:
Leptothrix
, .Didymohelix ferruginea
.4 :Mydrogenobacterria
:
Hydrogenomonas Ruhland
,
CO2 :
.5 :Methanobacteria
81
microbiology
,
Bacillus methanicus
,CO2 CO2
( ) ,
Methanococcus .
, 5%
,
.
- :Catabolism
,
,
.
,
.
.A :
,
,
,Aerobic Respiration
Fermentation ,Anaerobic Respiration
.
.a :
:
62 :
82
microbiology
Glycolysis
CH3-CO-COOH Krebs cycle
, CO2
The Citrice acid cycle
, ATP
,
CH3-CO-COOH
CO2 .H2O
GLYCOLYSIS
GLUCOSE
2ATP
2Pyrovic
acid
2NADH
H
2NADH
H
2CO2
+2H
step
2Acetyl CoA
Prearatory
83
microbiology
6NADH
H
+6H
4CO2
KREBS
2FADH2
CYCLE
6CO2
+H
2ATP
10NADH
2FADH2
)(TOTAL
Electrons
34ATP
+12H
62
38ATP
6O 2
6H2O
) ,(38ATP
,
( NAD FAD NADH )FADH .
.b :Fermentation
,
,
,
:
.i :Alcoholic fermentation
.
.Klebsiella aerogenes
.ii
:Homofermentative bacteria
84
microbiology
Lactic Acid
Lactobacillus lactis L.casei
.Streptococcus lactis
.iii :Heterofermentative
() Succinic acid
, , ,
:
Propionibacterium ,
.
.v :Butyric fermentation
( Butyric acid)
:
Clostridium C.butyricum
C.butylicum C.acetobutylicum
.
.vi :Enterobacteriaceae
( Formic acid)
H-COOH CH3-(COOH)2-CH3 .
85
microbiology
,
,
Adenosine tri phosphate ATP
( - 6 -)
.63
ATP +
- 6- ADP+
-1-
+
+
2ATP
2H
-6-
-6-1-
3
2H
CO2
86
microbiology
+
2ATP
2H
)(E.M.P
)(E.D
((H.M.P
63
.
- :
Clostridium pasteurinum
, C.pectinovorum
,
,
Methanobacterium .CH4
.B :
,
.
.a
, ,
Azotobacter Spirillum -Clostridium
.Rhizobium
87
microbiology
.b
:
:
N2
.c
():
,
Micrococcus Proteus vulgarism ,
Deamination Decarboxylation CO2
() :
,
) (ONH2 ).(-NH2
.
().
,
.
.d
:
()
,
Nitrosomonas Nitrobacter ,
Nitrosocystes ocean .
.e
:Dinitrification
,
Chromobacterium Serratia
:
88
microbiology
Thiobacillus
.Clostridium
.C :
, Nocardia corllina Bacillus
megaterium ,
,Purines ATP ,Pyrimidines .
CO2 Ca3(PO4)2
) (Ca HPO4 .
,
.
.D :
, E.coli Bacillus subtilis
H2S ,
:
H2S
, CO2 H2S
.
Thiobacillus
.
microbiology
89