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  • Microbial Profiling of HIV Women's Vaginal Microbiota

    Transféré par

    Nur Insana Imaniar
    12 vues2 pages
    MICROBIAL PROFILING (METHOD and RESULTS) was performed on Vaginal Microbiota from HIV women. NGS technologies have an impressive range of applications, and more are being developed. Clustering of the large number of reads derived from Illumina sequencing would be more accurate if it took both the sequence variation and the underlying error rates into account.

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    Overview Microbial Profiling (Method and Results)

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    MICROBIAL PROFILING (METHOD and RESULTS) was performed on Vaginal Microbiota from HIV women. NGS technologies have an impressive range of applications, and more are being developed. Clustering of the large number of reads derived from Illumina sequencing would be more accurate if it took both the sequence variation and the underlying error rates into account.

    Droits d'auteur :

    Attribution Non-Commercial (BY-NC)
    Téléchargez comme PPTX, PDF, TXT ou lisez en ligne sur Scribd
    Signaler comme contenu inapproprié
    12 vues2 pages

    Microbial Profiling of HIV Women's Vaginal Microbiota

    Transféré par

    Nur Insana Imaniar
    MICROBIAL PROFILING (METHOD and RESULTS) was performed on Vaginal Microbiota from HIV women. NGS technologies have an impressive range of applications, and more are being developed. Clustering of the large number of reads derived from Illumina sequencing would be more accurate if it took both the sequence variation and the underlying error rates into account.

    Droits d'auteur :

    Attribution Non-Commercial (BY-NC)
    Téléchargez comme PPTX, PDF, TXT ou lisez en ligne sur Scribd
    Signaler comme contenu inapproprié
    sur 2

    OVERVIEW MICROBIAL PROFILING (METHOD AND RESULTS)

    Vaginal Microbiota from HIV women PCR V6 Region (110-130 nt) E.Coli primer (Probe macth RDP)+ Tag/adaptor (3-6 nt) Illumimna Sequencing (read 76 nt)

    Analysis Data (Mismatch Overlapp Region

    selection

    Individual Sequence Units (ISU)


    Chimeric Anlysis

    126832 sequence Sequence 100% Comparison with identity BLAST 1384

    Clustering By Operational Taxonomic Units (OTU)

    CONCLUSIONS
    NGS technologies have an impressive range of applications, and more are being developed. In addition to the applications described above, NGS technologies are being used to characterize the evolutionary relationships of ancient genomes and to elucidate the role of noncoding RNAs in health and disease. Sequence clustering of the large number of reads derived from Illumina sequencing would be more accurate if it took both the sequence variation and the underlying error rates into account.

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