Vi = f ([S]) et 1/Vi = f (1/[S])

NOM:

tube [oNPG] A415 vi 1/[S] 1/vi

µmol/mL pour 3 min (µmol/mL/min) mL/µmol min.mL/µmol
0 0 0 0
1 0.05 0.25 0 20.0 41220
2 0.1 0.55 0 10.0 18736
3 0.15 0.75 0 6.7 13740
4 0.2 0.85 0 5.0 12124
5 0.25 0.92 0 4.0 11201
6 0.3 0.96 0 3.3 10734
7 0.35 0.99 0 2.9 10409
8 0.4 1 0 2.5 10305

42500
40000
37500
35000
32500
30000

1 / [oNPG]
27500
25000
22500
20000
17500
15000
12500
10000
2.5 5.0 7
 Vi = f([S])
0
0
0
Vi (µmol/mL/min)

0
0
0
0
0
0
0
0
0 0.05 0.1 0.15 0.2 0.25 0.3 0.35 0.4
[oNPG] (µmol/mL)

Représentation de Lineweaver-Burk
42500
40000
37500
35000
32500
30000
1 / [oNPG]

27500 Column G
25000 Linear regression,
22500 Column G

20000
17500
15000
12500
10000
2.5 5.0 7.5 10.0 12.5 15.0 17.5 20.0
1 / Vi