Faculty of Agriculture, Kasetsart University

Department of Entomology, Bangkhen, Bangkok 10900 Thailand

Microbial Control of Insect Laboratory

Under the direction of

Assoc. Prof.Jariya Chanpaisaeng, Ph.D. (Entomology)
University of the Philippines at LosBanos agrjyc@ku.ac.th

Our laboratory has focused on application of Bacillus thuringiensis

(Bt) in microbial control of insect pest which importantly related to
national policy in reduction of chemical pesticides use and utilization of
natural resources
Research interest

- Biodiversity of Bt in Thailand.
- Collection, isolation and identification to Bt subspecies (strains).
- Screening bioassay of high-efficiency Bt strains in economically Bt Research Team
Dr. Tipvadee Attathom
important pests such as diamondback moth and beet armyworm.
Dr. Suttipan Keawsompong
- Molecular biology study on cry type genes of Bt and the use of Dr. Yupa Pankaew
specific (toxic-producing) cry gene type detection. Dr. Nutchanat Tungjitsomkitkul
- Study on optimal condition for lab scale and pilot scale Bt
production.
CP

CP

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Research outcomes
- Field trial and farmer field application
- Technology transfer of Bt application to farmers.
On-going research
1. Use of polymerase chain reaction to detect cry genes in
Bacillus thuringiensis of Thai isolates with efficiency
in controlling coleopteran and dipteran insect pest.
2. Exclusion of Bacillus thuringiensis producing exotoxin by
PCR.
3. Development of concentrate Bt production at pilot scale.
4. Pilot plant development of Bt and nematode production for
insect pest control in farming community.
5. Application of Thai strain Bacillus thuringiensis product for
controlling agarwood leaf eating caterpillar (Heortia
vitessoidea, O. Lepidoptera F. Crambidae

Past Students:
Mrs. Laojana chaowanadisai,
Mrs chaowanadisai Ph.D
Ph D
Ms. Wanaporn Wongnikong, M.Sc.
Ms. Tunlaya Klubkaew, M.Sc.

Current Students:
Ms. Prakai thaphan, Ph.D student
Ms. Ratsamee Promto, M.Sc. student

Publication
1. Poojitkanont, Y., S. Keawsompong and J. Chanpaisaeng. 2008. Identification and Characterization of cry Genes
Coding for Insecticidal Crystal Protein in Bacillus thuringiensis JC590. Kasetsart J. (Nat. Sci). 42: 51-60.
2. Thaphan, P., S.K. Keawsompong and J. Chanpaisaeng. 2008. Isolation, toxicity and detection of cry gene in
Bacillus thuringiensis isolates in Krabi province, Thailand. Songklanakarin J. Sci. Technol. 30(5): 597-601.