SAMPLE COLLECTION & STORAGE IN MOLECULAR PATHOLOGY

PRESENTED BY NAZNEEN ISLAM

WHAT IS A SAMPLE ?
ANY MATERIAL OR PARTICLE RECEIVED IN LABORATORY FOR THE JUDGEMENT OF PHYSICAL OR CHEMICAL PROPERTIES IS KNOWN AS SAMPLE TYPES OF SAMPLE BLOOD BONE MARROW BODY FLUIDS CSF SPUTUM BIOPSY URINE SWAB

PURPOSE OF SENDING SAMPLE IN CLINICAL LABORATRY?

DIAGNOSIS OF DISEASE
BIOCHEMICAL ANALYSIS MICROBIOLOGICAL ANALYSIS

HEMATOLOGICAL PARAMETER
HISTOPATHOLOGY BLOOD BANK MOLECULAR BASED TESTING

AIM OF THIS LECTURE IS TO FAMILIARIZE THE NEW COMMERE REGARDING IMPORTANCE OF SAMPLE COLLECTION IN MOLECULAR DIAGNOSIS

WHY WE ARE GIVING IMPORTANCE TO SAMPLE STORAGE ?

DNA/RNA TEMPRATURE SENSITIVE

FEAR TO BEING DIGEST BY RNase/DNase FALSE NEGATIVE RESULTS QUALITY CARE TO PATIENTS LOSS OF SPECIMEN

What are the requirements for patient preparation, specimen collection, processing, and storage?
For each sample to be tested, the laboratory must establish methods for: The preparation of patients prior to phlebotomy specimen collection specimen labeling and assurance of positive identification specimen rejection (e.g., improper labeling, insufficient quantity, improper collection) specimen preservation prior to and after testing conditions for specimen transportation optimum integrity of specimens from the time of collection until testing has been completed and results reported

MOLECULAR DIAGNOSIS

VIROLOGY HLA TEST ONCOLOGY GENETICS BACTERIOLOGY

VIROLOGY
Sr# NAME OF TEST Sample Required Collection container Temp.

Hepatitis C Qualitative

BLOOD 10 CC

SERUM

GEL TUBE

Freeze

Hepatitis C Quantitative

BLOOD 5 CC

SERUM

GEL TUBE

Freeze

Hepatitis B Qualitative

BLOOD 5 CC

SERUM

GEL TUBE

Freeze

Hepatitis B Quantitative

BLOOD 5 CC

SERUM

GEL TUBE

Freeze

Cytomegalovirus

BLOOD 5 CC

WBCs

EDTA

4C

Herpes Simplex virus

CSF 1 ML

CSF

Syringe/Green

Freeze

Dengue IgM

BLOOD 5 CC

SERUM

GEL TUBE

Freeze

VIROLOGY
Sr#
8

NAME OF TEST
CCHF PCR

Sample
BLOOD 5 CC

Required
SERUM

Collection container
GEL TUBE

Temp.
Freeze

HCV RNA Genotyping

BLOOD 5 CC

SERUM

GEL TUBE

Freeze

10

HIV QUALITATIVE

BLOOD 5 CC

PLASMA

EDTA

Freeze

11

HIV QUANTITATIVE

BLOOD 5 CC

PLASMA

EDTA

Freeze

12

HDV PCR

BLOOD 5 CC

SERUM

GEL TUBE

Freeze

13

QUANTITATIVE HDV

BLOOD 5 CC

SERUM

GEL TUBE

Freeze

14

HBV Genotyping

BLOOD 5 CC

SERUM

GEL TUBE

Freeze

VIROLOGY
Sr# NAME OF TEST Sample Required
Collection container

Temp.
Freeze

15

Swine Flu H1N1

Nasal swab

UTM

16

Hepatitis E by PCR

BLOOD 5 CC

SERUM

GEL TUBE

Freeze

HLA TEST
Sr# NAME OF TEST Sample Required

Collection container

Temp.

17

TOTAL HLA TYPING

BLOOD 20 CC

WBCs

EDTA

4C

18

HLA TYPING MINUS DR BLOOD 20 CC

WBCs

EDTA

4C

19

HLA DR

BLOOD 10 CC

WBCs

EDTA

4C

20

HLA B-27

BLOOD 10 CC

WBCs

EDTA

4C

ONCOLOGY
Sr# NAME OF TEST Sample Required Collection container Temp.

21

BCR-abl Translocation

BLOOD 10 CC

WBCs

EDTA

4C

22

15,17 Translocation

BLOOD 5 CC

WBCs

EDTA

4C

23

Quantitative BCR-abl

BLOOD 10 CC

WBCs

EDTA

4C

24

BCR-abl Translocation

BLOOD 10 CC

WBCs

EDTA

4C

25

15,17 Translocation

BLOOD 5 CC
BLOOD 5 CC or IML BOME MARROW

WBCs

EDTA

4C

26

FLT3 by PCR

WBCs

EDTA

4C

GENETICS
Sr#
27

NAME OF TEST Sample

Required

Collection container
EDTA

Temp.
4C

Thalessemia in blood BLOOD 10CC(M,F) WBCs Thalessemia PCR (CVS)

28

BLOOD 10CC(M,F) WBCs

EDTA

4C

29

Cystic Fibrosis

BLOOD 5 CC

WBCs

EDTA

4C

30

DNA Banking

BLOOD 5 CC

WBCs

EDTA

4C

31

QPRE (Pat,Don)

BLOOD 5 CC

WBCs

EDTA

4C

32

QPOST

BLOOD 5 CC

WBCs

EDTA

4C

33

SMA

BLOOD 5 CC

WBCs

EDTA

4C

GENETICS
Sr# NAME OF TEST Sample Required Collection container Temp.

34

JAK2

BLOOD 5 CC

WBCs

EDTA

4C

35

MTHFR Polymorphism

BLOOD 5 CC

WBCs

EDTA

4C

36

DMD by MLPA

BLOOD 5 CC

WBCs

EDTA

4C

37

Prader Willi and Angelman Syndrom

BLOOD 5 CC

WBCs

EDTA

4C

Bacteriology/Parasitology
Sr# NAME OF TEST Sample Required
Collection container

Temp.

38

AFB PCR

BLOOD 10 CC

WBCs

EDTA

4C

Sputum, Body Fluids

Sterile Container

Freezing

39

CTA

Urine

Green top

4C

40

Naegleria Sp PCR

CSF

Sterile Container

Freezing

MOLECULAR DIAGNOSIS WORKING MAP

SET UP SAVE IN -80 OR -20 FREEZER

DNA/RNA EXTRACTION

MASTER MIX AREA (MM)

MM + RNA OR DNA

PCR AREA
POST PCR DETECTION

SAVE TARGET DNA / RNA DURING SAMPLE PROCESSING

IMPROPER HANDLING MAY LEAD TO THE LOSS OF TARGET DNA/RNA

THANK YOU