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pDsRed2-Peroxi

CMV IE Promoter

AseI
AseI

1 TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG ATCAATAATT ATCATTAGTT AATGCCCCAG TAATCAAGTA TCGGGTATAT ACCTCAAGGC

CMV IE Promoter

61 CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT GCAATGTATT GAATGCCATT TACCGGGCGG ACCGACTGGC
61 CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT
GCAATGTATT GAATGCCATT TACCGGGCGG ACCGACTGGC GGGTTGCTGG GGGCGGGTAA

CMV IE Promoter

121 GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA CTGCAGTTAT TACTGCATAC AAGGGTATCA TTGCGGTTAT
121 GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA
CTGCAGTTAT TACTGCATAC AAGGGTATCA TTGCGGTTAT CCCTGAAAGG TAACTGCAGT

CMV IE Promoter

181 ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC TACCCACCTC ATAAATGCCA TTTGACGGGT GAACCGTCAT
181 ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC
TACCCACCTC ATAAATGCCA TTTGACGGGT GAACCGTCAT GTAGTTCACA TAGTATACGG

CMV IE Promoter

241 AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA TTCATGCGGG GGATAACTGC AGTTACTGCC ATTTACCGGG
241 AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA
TTCATGCGGG GGATAACTGC AGTTACTGCC ATTTACCGGG CGGACCGTAA TACGGGTCAT

CMV IE Promoter

301 CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC GTACTGGAAT ACCCTGAAAG GATGAACCGT CATGTAGATG
301 CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC
GTACTGGAAT ACCCTGAAAG GATGAACCGT CATGTAGATG CATAATCAGT AGCGATAATG

CMV IE Promoter

361 CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG GTACCACTAC GCCAAAACCG TCATGTAGTT ACCCGCACCT
361 CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG
GTACCACTAC GCCAAAACCG TCATGTAGTT ACCCGCACCT ATCGCCAAAC TGAGTGCCCC

CMV IE Promoter

421 ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG TAAAGGTTCA GAGGTGGGGT AACTGCAGTT ACCCTCAAAC
421 ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG
TAAAGGTTCA GAGGTGGGGT AACTGCAGTT ACCCTCAAAC AAAACCGTGG TTTTAGTTGC

CMV IE Promoter

481 GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT CCTGAAAGGT TTTACAGCAT TGTTGAGGCG GGGTAACTGC
481 GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT
CCTGAAAGGT TTTACAGCAT TGTTGAGGCG GGGTAACTGC GTTTACCCGC CATCCGCACA

CMV IE Promoter

541 ACGGTGGGAG GTCTATATAA GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCGCTA TGCCACCCTC CAGATATATT CGTCTCGACC AAATCACTTG
541 ACGGTGGGAG GTCTATATAA GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCGCTA
TGCCACCCTC CAGATATATT CGTCTCGACC AAATCACTTG GCAGTCTAGG CGATCGCGAT

DsRed2-PTS1 fusion

DsRed2

601 CCGGTCGCCA CCATGGCCTC CTCCGAGAAC GTCATCACCG AGTTCATGCG CTTCAAGGTG GGCCAGCGGT GGTACCGGAG GAGGCTCTTG CAGTAGTGGC
601 CCGGTCGCCA CCATGGCCTC CTCCGAGAAC GTCATCACCG AGTTCATGCG CTTCAAGGTG
GGCCAGCGGT GGTACCGGAG GAGGCTCTTG CAGTAGTGGC TCAAGTACGC GAAGTTCCAC

DsRed2-PTS1 fusion

DsRed2

661 CGCATGGAGG GCACCGTGAA CGGCCACGAG TTCGAGATCG AGGGCGAGGG CGAGGGCCGC GCGTACCTCC CGTGGCACTT GCCGGTGCTC AAGCTCTAGC
661 CGCATGGAGG GCACCGTGAA CGGCCACGAG TTCGAGATCG AGGGCGAGGG CGAGGGCCGC
GCGTACCTCC CGTGGCACTT GCCGGTGCTC AAGCTCTAGC TCCCGCTCCC GCTCCCGGCG

DsRed2-PTS1 fusion

DsRed2

721 CCCTACGAGG GCCACAACAC CGTGAAGCTG AAGGTGACCA AGGGCGGCCC CCTGCCCTTC GGGATGCTCC CGGTGTTGTG GCACTTCGAC TTCCACTGGT
721 CCCTACGAGG GCCACAACAC CGTGAAGCTG AAGGTGACCA AGGGCGGCCC CCTGCCCTTC
GGGATGCTCC CGGTGTTGTG GCACTTCGAC TTCCACTGGT TCCCGCCGGG GGACGGGAAG

DsRed2-PTS1 fusion

DsRed2

781 GCCTGGGACA TCCTGTCCCC CCAGTTCCAG TACGGCTCCA AGGTGTACGT GAAGCACCCC CGGACCCTGT AGGACAGGGG GGTCAAGGTC ATGCCGAGGT
781 GCCTGGGACA TCCTGTCCCC CCAGTTCCAG TACGGCTCCA AGGTGTACGT GAAGCACCCC
CGGACCCTGT AGGACAGGGG GGTCAAGGTC ATGCCGAGGT TCCACATGCA CTTCGTGGGG

DsRed2-PTS1 fusion

DsRed2

841 GCCGACATCC CCGACTACAA GAAGCTGTCC TTCCCCGAGG GCTTCAAGTG GGAGCGCGTG CGGCTGTAGG GGCTGATGTT CTTCGACAGG AAGGGGCTCC
841 GCCGACATCC CCGACTACAA GAAGCTGTCC TTCCCCGAGG GCTTCAAGTG GGAGCGCGTG
CGGCTGTAGG GGCTGATGTT CTTCGACAGG AAGGGGCTCC CGAAGTTCAC CCTCGCGCAC

DsRed2-PTS1 fusion

DsRed2

901 ATGAACTTCG AGGACGGCGG CGTGGCGACC GTGACCCAGG ACTCCTCCCT GCAGGACGGC TACTTGAAGC TCCTGCCGCC GCACCGCTGG CACTGGGTCC
901 ATGAACTTCG AGGACGGCGG CGTGGCGACC GTGACCCAGG ACTCCTCCCT GCAGGACGGC
TACTTGAAGC TCCTGCCGCC GCACCGCTGG CACTGGGTCC TGAGGAGGGA CGTCCTGCCG

DsRed2-PTS1 fusion

DsRed2

961 TGCTTCATCT ACAAGGTGAA GTTCATCGGC GTGAACTTCC CCTCCGACGG CCCCGTGATG ACGAAGTAGA TGTTCCACTT CAAGTAGCCG CACTTGAAGG
961 TGCTTCATCT ACAAGGTGAA GTTCATCGGC GTGAACTTCC CCTCCGACGG CCCCGTGATG
ACGAAGTAGA TGTTCCACTT CAAGTAGCCG CACTTGAAGG GGAGGCTGCC GGGGCACTAC

DsRed2-PTS1 fusion

DsRed2

StuI
StuI

1021 CAGAAGAAGA CCATGGGCTG GGAGGCCTCC ACCGAGCGCC TGTACCCCCG CGACGGCGTG GTCTTCTTCT GGTACCCGAC CCTCCGGAGG TGGCTCGCGG ACATGGGGGC GCTGCCGCAC

pDsRed2-Peroxi

DsRed2-PTS1 fusion

DsRed2

EcoO109I
EcoO109I

1081 CTGAAGGGCG AGACCCACAA GGCCCTGAAG CTGAAGGACG GCGGCCACTA CCTGGTGGAG GACTTCCCGC TCTGGGTGTT CCGGGACTTC GACTTCCTGC CGCCGGTGAT GGACCACCTC

DsRed2-PTS1 fusion

DsRed2

1141 TTCAAGTCCA TCTACATGGC CAAGAAGCCC GTGCAGCTGC CCGGCTACTA CTACGTGGAC AAGTTCAGGT AGATGTACCG GTTCTTCGGG CACGTCGACG
1141 TTCAAGTCCA TCTACATGGC CAAGAAGCCC GTGCAGCTGC CCGGCTACTA CTACGTGGAC
AAGTTCAGGT AGATGTACCG GTTCTTCGGG CACGTCGACG GGCCGATGAT GATGCACCTG

DsRed2-PTS1 fusion

DsRed2

1201 GCCAAGCTGG ACATCACCTC CCACAACGAG GACTACACCA TCGTGGAGCA GTACGAGCGC CGGTTCGACC TGTAGTGGAG GGTGTTGCTC CTGATGTGGT
1201 GCCAAGCTGG ACATCACCTC CCACAACGAG GACTACACCA TCGTGGAGCA GTACGAGCGC
CGGTTCGACC TGTAGTGGAG GGTGTTGCTC CTGATGTGGT AGCACCTCGT CATGCTCGCG

DsRed2-PTS1 fusion

DsRed2

Peroxisomal Targeting Signal (P NotI

fusion DsRed2 Peroxisomal Targeting Signal (P NotI 1261 ACCGAGGGCC GCCACCACCT GTTCCTGAGA TCGTACAAGT

1261

ACCGAGGGCC GCCACCACCT GTTCCTGAGA TCGTACAAGT CCAAGCTGTA GCGGCCGCGA TGGCTCCCGG CGGTGGTGGA CAAGGACTCT AGCATGTTCA GGTTCGACAT CGCCGGCGCT

 
XbaI
XbaI

1321

CTCTAGATCA TAATCAGCCA TACCACATTT GTAGAGGTTT TACTTGCTTT AAAAAACCTC GAGATCTAGT ATTAGTCGGT ATGGTGTAAA CATCTCCAAA ATGAACGAAA TTTTTTGGAG

1381

CCACACCTCC CCCTGAACCT GAAACATAAA ATGAATGCAA TTGTTGTTGT TAACTTGTTT GGTGTGGAGG GGGACTTGGA CTTTGTATTT TACTTACGTT AACAACAACA ATTGAACAAA

 

SV40 early polyA sig

SV40 early polyA sig

1441

ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TAACGTCGAA TATTACCAAT GTTTATTTCG TTATCGTAGT GTTTAAAGTG TTTATTTCGT

 
AflII f1 origi
AflII
f1 origi

1501

TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTAAGGCGTA AAAAAAAGTG ACGTAAGATC AACACCAAAC AGGTTTGAGT AGTTACATAG AATTCCGCAT

f1 origin 1561 AATTGTAAGC GTTAATATTT TGTTAAAATT CGCGTTAAAT TTTTGTTAAA TCAGCTCATT TTAACATTCG CAATTATAAA ACAATTTTAA
f1 origin
1561
AATTGTAAGC GTTAATATTT TGTTAAAATT CGCGTTAAAT TTTTGTTAAA TCAGCTCATT
TTAACATTCG CAATTATAAA ACAATTTTAA GCGCAATTTA AAAACAATTT AGTCGAGTAA
f1 origin
1621
TTTTAACCAA TAGGCCGAAA TCGGCAAAAT CCCTTATAAA TCAAAAGAAT AGACCGAGAT
AAAATTGGTT ATCCGGCTTT AGCCGTTTTA GGGAATATTT AGTTTTCTTA TCTGGCTCTA
f1 origin
1681
AGGGTTGAGT GTTGTTCCAG TTTGGAACAA GAGTCCACTA TTAAAGAACG TGGACTCCAA
TCCCAACTCA CAACAAGGTC AAACCTTGTT CTCAGGTGAT AATTTCTTGC ACCTGAGGTT
f1 origin
1741
CGTCAAAGGG CGAAAAACCG TCTATCAGGG CGATGGCCCA CTACGTGAAC CATCACCCTA
GCAGTTTCCC GCTTTTTGGC AGATAGTCCC GCTACCGGGT GATGCACTTG GTAGTGGGAT
f1 origin
1801
ATCAAGTTTT TTGGGGTCGA GGTGCCGTAA AGCACTAAAT CGGAACCCTA AAGGGAGCCC
TAGTTCAAAA AACCCCAGCT CCACGGCATT TCGTGATTTA GCCTTGGGAT TTCCCTCGGG
f1 origin
1861
CCGATTTAGA GCTTGACGGG GAAAGCCGGC GAACGTGGCG AGAAAGGAAG GGAAGAAAGC
GGCTAAATCT CGAACTGCCC CTTTCGGCCG CTTGCACCGC TCTTTCCTTC CCTTCTTTCG
f1 origin
1921
GAAAGGAGCG GGCGCTAGGG CGCTGGCAAG TGTAGCGGTC ACGCTGCGCG TAACCACCAC
CTTTCCTCGC CCGCGATCCC GCGACCGTTC ACATCGCCAG TGCGACGCGC ATTGGTGGTG
f1 origin
1981
ACCCGCCGCG CTTAATGCGC CGCTACAGGG CGCGTCAGGT GGCACTTTTC GGGGAAATGT
TGGGCGGCGC GAATTACGCG GCGATGTCCC GCGCAGTCCA CCGTGAAAAG CCCCTTTACA
Bacterial promoter for Kan(R)
f1 origin
2041
GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG
CGCGCCTTGG GGATAAACAA ATAAAAAGAT TTATGTAAGT TTATACATAG GCGAGTACTC
Bacterial promoter for Kan
f1 origin

2101

ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTCCTG AGGCGGAAAG TGTTATTGGG ACTATTTACG AAGTTATTAT AACTTTTTCC TTCTCAGGAC TCCGCCTTTC

2161

SV40 early Promoter/Enhancer

AACCAGCTGT GGAATGTGTG TCAGTTAGGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC TTGGTCGACA CCTTACACAC AGTCAATCCC ACACCTTTCA GGGGTCCGAG GGGTCGTCCG

pDsRed2-Peroxi

SV40 early Promoter/Enhancer

2221 AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA GGTGTGGAAA GTCCCCAGGC TCTTCATACG TTTCGTACGT AGAGTTAATC AGTCGTTGGT
2221
AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA GGTGTGGAAA GTCCCCAGGC
TCTTCATACG TTTCGTACGT AGAGTTAATC AGTCGTTGGT CCACACCTTT CAGGGGTCCG
SV40 early Promoter/Enhancer
2281
TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CATAGTCCCG
AGGGGTCGTC CGTCTTCATA CGTTTCGTAC GTAGAGTTAA TCAGTCGTTG GTATCAGGGC
SV40 origin
SV40 early Promoter/Enhancer
2341
CCCCTAACTC CGCCCATCCC GCCCCTAACT CCGCCCAGTT CCGCCCATTC TCCGCCCCAT
GGGGATTGAG GCGGGTAGGG CGGGGATTGA GGCGGGTCAA GGCGGGTAAG AGGCGGGGTA

SV40 origin

SV40 early Promoter/Enhancer

2401

GGCTGACTAA TTTTTTTTAT TTATGCAGAG GCCGAGGCCG CCTCGGCCTC TGAGCTATTC CCGACTGATT AAAAAAAATA AATACGTCTC CGGCTCCGGC GGAGCCGGAG ACTCGATAAG

2461

SV40 origin StuI
SV40 origin
StuI

CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC CTAGGCTTTT GCAAAGATCG ATCAAGAGAC GTCTTCATCA CTCCTCCGAA AAAACCTCCG GATCCGAAAA CGTTTCTAGC TAGTTCTCTG

2521

Kan(R)/Neo(R)

AGGATGAGGA TCGTTTCGCA TGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TCCTACTCCT AGCAAAGCGT ACTAACTTGT TCTACCTAAC GTGCGTCCAA GAGGCCGGCG

Kan(R)/Neo(R) 2581 TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC AACCCACCTC TCCGATAAGC CGATACTGAC
Kan(R)/Neo(R)
2581
TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC
AACCCACCTC TCCGATAAGC CGATACTGAC CCGTGTTGTC TGTTAGCCGA CGAGACTACG
Kan(R)/Neo(R)
2641
CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC
GCGGCACAAG GCCGACAGTC GCGTCCCCGC GGGCCAAGAA AAACAGTTCT GGCTGGACAG
Kan(R)/Neo(R)
2701
CGGTGCCCTG AATGAACTGC AAGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG
GCCACGGGAC TTACTTGACG TTCTGCTCCG TCGCGCCGAT AGCACCGACC GGTGCTGCCC
Kan(R)/Neo(R)
2761
CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT
GCAAGGAACG CGTCGACACG AGCTGCAACA GTGACTTCGC CCTTCCCTGA CCGACGATAA
Kan(R)/Neo(R)
2821
GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC
CCCGCTTCAC GGCCCCGTCC TAGAGGACAG TAGAGTGGAA CGAGGACGGC TCTTTCATAG
Kan(R)/Neo(R)
2881
CATCATGGCT GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA
GTAGTACCGA CTACGTTACG CCGCCGACGT ATGCGAACTA GGCCGATGGA CGGGTAAGCT
Kan(R)/Neo(R)
2941
CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA
GGTGGTTCGC TTTGTAGCGT AGCTCGCTCG TGCATGAGCC TACCTTCGGC CAGAACAGCT
Kan(R)/Neo(R)
3001
TCAGGATGAT CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT
AGTCCTACTA GACCTGCTTC TCGTAGTCCC CGAGCGCGGT CGGCTTGACA AGCGGTCCGA
Kan(R)/Neo(R)
3061
CAAGGCGAGC ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC
GTTCCGCTCG TACGGGCTGC CGCTCCTAGA GCAGCACTGG GTACCGCTAC GGACGAACGG
Kan(R)/Neo(R)
3121
GAATATCATG GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT
CTTATAGTAC CACCTTTTAC CGGCGAAAAG ACCTAAGTAG CTGACACCGG CCGACCCACA
Kan(R)/Neo(R)
3181
GGCGGACCGC TATCAGGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG AGCTTGGCGG
CCGCCTGGCG ATAGTCCTGT ATCGCAACCG ATGGGCACTA TAACGACTTC TCGAACCGCC
Kan(R)/Neo(R)
3241
CGAATGGGCT GACCGCTTCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT CGCAGCGCAT
GCTTACCCGA CTGGCGAAGG AGCACGAAAT GCCATAGCGG CGAGGGCTAA GCGTCGCGTA
Kan(R)/Neo(R)
3301
CGCCTTCTAT CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA CTCTGGGGTT CGAAATGACC
GCGGAAGATA GCGGAAGAAC TGCTCAAGAA GACTCGCCCT GAGACCCCAA GCTTTACTGG
3361
GACCAAGCGA CGCCCAACCT GCCATCACGA GATTTCGATT CCACCGCCGC CTTCTATGAA
CTGGTTCGCT GCGGGTTGGA CGGTAGTGCT CTAAAGCTAA GGTGGCGGCG GAAGATACTT
3421
AGGTTGGGCT TCGGAATCGT TTTCCGGGAC GCCGGCTGGA TGATCCTCCA GCGCGGGGAT
TCCAACCCGA AGCCTTAGCA AAAGGCCCTG CGGCCGACCT ACTAGGAGGT CGCGCCCCTA

pDsRed2-Peroxi

3481

CTCATGCTGG AGTTCTTCGC CCACCCTAGG GGGAGGCTAA CTGAAACACG GAAGGAGACA GAGTACGACC TCAAGAAGCG GGTGGGATCC CCCTCCGATT GACTTTGTGC CTTCCTCTGT

 

HSV TK polyA sig

HSV TK polyA sig

3541

ATACCGGAAG GAACCCGCGC TATGACGGCA ATAAAAAGAC AGAATAAAAC GCACGGTGTT TATGGCCTTC CTTGGGCGCG ATACTGCCGT TATTTTTCTG TCTTATTTTG CGTGCCACAA

3601

GGGTCGTTTG TTCATAAACG CGGGGTTCGG TCCCAGGGCT GGCACTCTGT CGATACCCCA CCCAGCAAAC AAGTATTTGC GCCCCAAGCC AGGGTCCCGA CCGTGAGACA GCTATGGGGT

3661

CCGAGACCCC ATTGGGGCCA ATACGCCCGC GTTTCTTCCT TTTCCCCACC CCACCCCCCA GGCTCTGGGG TAACCCCGGT TATGCGGGCG CAAAGAAGGA AAAGGGGTGG GGTGGGGGGT

 
EcoO109I
EcoO109I

3721

AGTTCGGGTG AAGGCCCAGG GCTCGCAGCC AACGTCGGGG CGGCAGGCCC TGCCATAGCC TCAAGCCCAC TTCCGGGTCC CGAGCGTCGG TTGCAGCCCC GCCGTCCGGG ACGGTATCGG

3781

TCAGGTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC AGTCCAATGA GTATATATGA AATCTAACTA AATTTTGAAG TAAAAATTAA ATTTTCCTAG

3841

TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC ATCCACTTCT AGGAAAAACT ATTAGAGTAC TGGTTTTAGG GAATTGCACT CAAAAGCAAG

 

pUC origin

3901

CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG GTGACTCGCA GTCTGGGGCA TCTTTTCTAG TTTCCTAGAA GAACTCTAGG AAAAAAAGAC

pUC origin 3961 CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GCGCATTAGA CGACGAACGT TTGTTTTTTT
pUC origin
3961
CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG
GCGCATTAGA CGACGAACGT TTGTTTTTTT GGTGGCGATG GTCGCCACCA AACAAACGGC
pUC origin
4021
GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA
CTAGTTCTCG ATGGTTGAGA AAAAGGCTTC CATTGACCGA AGTCGTCTCG CGTCTATGGT
pUC origin
4081
AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG
TTATGACAGG AAGATCACAT CGGCATCAAT CCGGTGGTGA AGTTCTTGAG ACATCGTGGC
pUC origin
4141
CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG
GGATGTATGG AGCGAGACGA TTAGGACAAT GGTCACCGAC GACGGTCACC GCTATTCAGC
pUC origin
4201
TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA
ACAGAATGGC CCAACCTGAG TTCTGCTATC AATGGCCTAT TCCGCGTCGC CAGCCCGACT
pUC origin
4261
ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC
TGCCCCCCAA GCACGTGTGT CGGGTCGAAC CTCGCTTGCT GGATGTGGCT TGACTCTATG
pUC origin
4321
CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT
GATGTCGCAC TCGATACTCT TTCGCGGTGC GAAGGGCTTC CCTCTTTCCG CCTGTCCATA
pUC origin
4381
CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC
GGCCATTCGC CGTCCCAGCC TTGTCCTCTC GCGTGCTCCC TCGAAGGTCC CCCTTTGCGG
pUC origin
4441
TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA
ACCATAGAAA TATCAGGACA GCCCAAAGCG GTGGAGACTG AACTCGCAGC TAAAAACACT
pUC origin
4501
TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC
ACGAGCAGTC CCCCCGCCTC GGATACCTTT TTGCGGTCGT TGCGCCGGAA AAATGCCAAG
pUC origi
4561
CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG
GACCGGAAAA CGACCGGAAA ACGAGTGTAC AAGAAAGGAC GCAATAGGGG ACTAAGACAC
4621
GATAACCGTA TTACCGCCAT GCAT
CTATTGGCAT AATGGCGGTA CGTA