Isolation and Identification Technique

Sukemi NIM. 1204016011 Management of Natural Resources and Renewable Energy Faculty of Forestry Mulawarman University 2013

GOALS
To investigate the antioxidant potential of polyphenolic rich ethyl acetate extract/fractions of Acacia auriculiformis A. Cunn employing DPPH radical-scavenging, reducing power, Fe2+ chelating ability and lipid peroxidation assays

METHODS
EXTRACTION AND FRACTINATION BY INCREASING ORDER OF SOLVENT POLARITY

METHODS
EXTRACTION AND FRACTINATION BY DECREASING ORDER OF SOLVENT POLARITY

METHODS
Determination of total phenolics Antioxidant testing assays DPPH scavenging assay Reducing power assay

Deoxyribose degradation assay Chelating effects on ferrous ions

Lipid peroxidation by thiobarbituric acid (TBA) Assay Statistical analysis

RESULTS
TOTAL FENOLIC CONTENT

Water fraction has a higher phenolic content (720 mg) than ethyl acetate fraction (600 mg) and crude ethyl acetate extract (390 mg) expressed as gallic acid equivalents (GAE)/g of extract/fraction
ANTIOXIDANT ACTIVITY DPPH SCAVENGING

ANTIOXIDANT ACTIVITY REDUCING POWER

NON SITE SPESIFIC

ANTIOXIDANT ACTIVITY SITE SPESIFIC

CHELATING POWER

ANTIOXIDANT ACTIVITY LIPID PEROXIDATION

The difference in the antioxidant activity of the water, ethyl acetate fractions and crude extract may be ascribed to the difference in the total phenolic content as well as the phenolic compositions.