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Papain treated R1R1 poolA Saline cards

Anti-D 73/515
Dilution 1:100 1:400 1:800 1:1600 1:3200 1:6400
Exp 1 4 3 2 1 1 0
Exp 2 4 3 3 2 1 1

Anti-D 86 i.u.
Dilution 1:400 1:1600 1:3200 1:6400 1:12800 1:25600
Exp 1 4 3 1 0 0 0
Exp 2 4 3 2 0 0 0

Anti-D lot 24311


Dilution 1:4 1:8 1:16 1:32 1:64 1:128
Exp 1 4 4 3 2 2 1
Exp 2 4 4 3 3 1 0

Anti-D Lot 32372B


Dilution 1:200 1:400 1:800 1:1600 1:3200 1:6400
Exp 1 4 4 3 3 1 0
Exp 2 4 4 3 2 1 0

Anti-D Lot 101


Dilution 1:50 1:100 1:200 1:400 1:800 1:1600
Exp 1 4 4 3 1 1 0
Exp 2 4 3 2 1 0 0

Anti-D Patient Pool A


Dilution Neat 1:2 1:4 1:8 1:16 1:32
Exp 1 4 3 1 0 0 0
Exp 2 4 2 1 0 0 0

Anti-D Lot 102


Dilution 1:100 1:200 1:400 1:800 1:1600 1:3200
Exp 1 4 3 3 1 0 0
Exp 2 4 2 1 0 0 0

Anti-D Lot 201


Dilution 1:100 1:200 1:400 1:800 1:1600 1:3200
Exp 1 4 4 3 1 0 0
Exp 2 4 3 2 0 0 0

Anti-D Lot 202


Dilution 1:100 1:200 1:400 1:800 1:1600 1:3200
Exp 1 3 1 0 0 0 0
Exp 2 3 0 0 0 0 0
Anti-D Lot 103
Dilution 1:100 1:200 1:400 1:800 1:1600 1:3200
Exp 1 4 4 3 3 1 0
Exp 2 4 4 3 3 1 0

Anti-D Lot 203


Dilution 1:200 1:400 1:800 1:1600 1:3200 1:6400
Exp 1 4 4 2 2 1 0
Exp 2 4 4 3 3 2 1
Pooled R1R1 cells Pool B
Cells Treated 14/2/09

Dilutions made in PBSS


Anti-D 73/515

Papainised cell Saline card Test date 19/2/09


Dilution 1:100 1:400 1:800 1:1600 1:3200 1:6400
Exp 1 4 4 3 2 1 0
Exp 2 4 4 3 2 0 0
Exp 3 4 4 3 2 0 0

Papainised cell AHG card Test date 19/2/09


Dilution 1:100 1:400 1:800 1:1600 1:3200 1:6400
Exp 1 4 4 3 2 2 1
Exp 2 4 4 3 3 2 0
Exp 3 4 4 3 3 1 0

Anti-D 86 i.u. Test date 19/2/09


Papainised cell Saline card Test date 19/2/09
Dilution 1:400 1:1600 1:3200 1:6400 1:12800 1:25600
Exp 1 4 3 2 1 0 0
Exp 2 4 3 2 0 0 0
Exp 3 4 4 3 1 0 0

Papainised cell AHG card Test date 19/2/09


Dilution 1:400 1:1600 1:3200 1:6400 1:12800 1:25600
Exp 1 4 4 3 1 0 0
Exp 2 4 4 4 1 2 2
Exp 3 4 4 3 2 1 0

Anti-D Lot 32372B


Papainised cell Saline card Test date 19/2/09
Dilution 1:200 1:400 1:800 1:1600 1:3200 1:6400
Exp 1 4 4 4 3 1 0
Exp 2 4 4 3 2 1 0
Exp 3 4 4 4 2 1 0

Papainised cell AHG card Test date 19/2/09


Dilution 1:200 1:400 1:800 1:1600 1:3200 1:6400 1:12800 1:25600
Exp 1 4 4 4 3 2 1
Exp 2 4 4 4 3 2 2
Exp 3 4 4 4 4 3 1
Exp 1 Dilutions made 4 4 3 1 0 0
Exp 2 and discarded 4 4 3 1 0 0
Exp 3 4 4 3 1 0 0
Anti-D Lot 203
Papainised cell Saline card Test date 19/2/09
Dilution 1:200 1:400 1:800 1:1600 1:3200 1:6400
Exp 1 4 4 3 3 1 0
Exp 2 4 4 3 3 0 0
Exp 3 4 4 4 3 0 0

Papainised cell AHG card Test date 19/2/09


Dilution 1:200 1:400 1:800 1:1600 1:3200 1:6400 1:12800 1:25600 1:51200
Exp 1 4 4 4 4 3 3
Exp 2 4 4 4 3 2 2
Exp 3 4 4 4 3 2 1
Exp 1 Dilutions made 4 3 1 0 0 0
Exp 2 and discarded 4 2 1 0 0 0
Exp 3 4 3 1 0 0 0
1:51200
Pooled R1R1 cells Pool B
Test Date 25/2/09 Cell treated 14/2/09
very weak non specific reactions
Dilutions made in Albumin saline

Anti-D 73/515
Papainised cell Saline card
Dilution 1:100 1:400 1:800 1:1600 1:3200 1:6400 1:12800 1:25600
Exp 1 Dilutions discarded 4 3 2 0 0 0
Exp 2 4 3 1 0 0 0
Exp 3 4 3 1 0 0 0

Papainised cell AHG card


Dilution 1:100 1:400 1:800 1:1600 1:3200 1:6400 1:12800 1:25600
Exp 1 Dilutions discarded 3 2 1 1 0 0
Exp 2 3 3 2 1 0 0
Exp 3 3 3 2 1 1 0

Anti-D 86 i.u.
Papainised cell Saline card
Dilution 1:400 1:1600 1:3200 1:6400 1:12800 1:25600 1:512001:102400
Exp 1 Dilutions discarded 4 2 1 1 0 0
Exp 2 4 3 2 0 0 0
Exp 3 4 3 2 1 0 0

Papainised cell AHG card


Dilution 1:400 1:1600 1:3200 1:6400 1:12800 1:25600 1:512001:102400
Exp 1 Dilutions discarded 4 3 2 2 1 0
Exp 2 4 3 2 1 0 0
Exp 3 4 3 2 1 1 0

Anti-D Lot 32372B


Papainised cell Saline card
Dilution 1:200 1:400 1:800 1:1600 1:3200 1:6400 1:12800 1:25600
Exp 1 Dilutions discarded 4 3 2 1 0 0
Exp 2 4 4 3 1 0 0
Exp 3 4 4 3 1 0 0

Papainised cell AHG card


Dilution 1:200 1:400 1:800 1:1600 1:3200 1:6400 1:12800 1:25600
Exp 1 Dilutions discarded 4 4 3 2 1 1
Exp 2 4 4 3 2 1 1
Exp 3 4 4 3 2 1 1

Anti-D Lot 203


Papainised cell Saline card
Dilution 1:200 1:400 1:800 1:1600 1:3200 1:6400 1:12800 1:25600
Exp 1 Dilutions discarded 4 4 3 1 0 0
Exp 2 4 3 3 2 1 1
Exp 3 4 3 2 1 1 0

Papainised cell AHG card


Dilution 1:200 1:400 1:800 1:1600 1:3200 1:6400 1:12800 1:25600
Exp 1 Dilutions discarded 4 4 3 2 2 1
Exp 2 4 4 4 3 2 1
Exp 3 4 4 4 3 2 1
Pooled R1R1 cells Pool B
Test date 19/2/09 Treated date 14/2/09

Dilutions made in AB serum

Anti-D 73/515
Papainised cell Saline card
Dilution 1:100 1:400 1:800 1:1600 1:3200 1:6400 1:12800
Exp 1 4 4 3 3 1 0
Exp 2 4 4 3 2 1 0
Exp 3 4 4 3 2 1 0

Papainised cell AHG card


Dilution 1:100 1:400 1:800 1:1600 1:3200 1:6400 1:12800
Exp 1 4 4 3 3 1 0
Exp 2 4 4 3 3 1 0
Exp 3 4 4 3 2 1 0

Anti-D 86 i.u.
Papainised cell Saline card
Dilution 1:400 1:1600 1:3200 1:6400 1:12800 1:25600
Exp 1 4 4 3 2 1 0
Exp 2 4 4 3 3 1 0
Exp 3 4 4 3 3 1 0

Papainised cell AHG card


Dilution 1:400 1:1600 1:3200 1:6400 1:12800 1:25600
Exp 1 4 4 4 3 2 2
Exp 2 4 4 4 3 2 1
Exp 3 4 4 4 3 2 1

Anti-D Lot 32372B


Papainised cell Saline card
Dilution 1:200 1:400 1:800 1:1600 1:3200 1:6400 1:12800 1:25600
Exp 1 Dilutions discarded 4 4 3 1 0 0
Exp 2 4 4 3 2 0 0
Exp 3 4 4 3 2 0 0

Papainised cell AHG card


Dilution 1:200 1:400 1:800 1:1600 1:3200 1:6400 1:12800 1:25600
Exp 1 Dilutions discarded 4 4 4 2 1 0
Exp 2 4 4 4 2 1 0
Exp 3 4 4 4 3 1 0

Anti-D Lot 203


Papainised cell Saline card
Dilution 1:200 1:400 1:800 1:1600 1:3200 1:6400 1:12800 1:25600
Exp 1 Dilutions discarded 4 4 3 2 1 0
Exp 2 4 3 3 2 0 0
Exp 3 4 3 3 2 0 0

Papainised cell AHG card


Dilution 1:200 1:400 1:800 1:1600 1:3200 1:6400 1:12800 1:25600
Exp 1 Dilutions discarded 4 4 3 3 1 1
Exp 2 4 4 3 2 1 1
Exp 3 4 4 3 2 1 1