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API strip reactions: ONPG o Detects enzyme: B-galactosidase o Products: orthonoitrophenol and galactose o Positive: yellow o Negative: colorless

s ADH, LDC, ODC Citrate o Carbon Source o Indicator: Bromothymol Blue o Blue is Positive H2S o Citrobacter o Salmonella o Edwardsiella o Proteus Urease o Enzyme: urease urea to ammonia o Indicator: Phenol red (alkaline is pos) o Positive: Pink (red/orange on API) o Negative: Yellow TDA: Tryptophane o Trytophane deaminase o Similar to PDA (phenylaleninedeaminase-PHE) o Reagent 10% ferric chloride o 5 minutes, wait o Positive: dark green (tube) Brown/red (API) o Negative: no change Tube Indole o Org uses enzume tryptophanase to produce indole o Reagent: Kovacs o Positive: red o Negative: yellow/no color MR/VP, Glucose Pathway o VP-Voges Proskauer API Butylene glycol pathway 1 drop VP A 1 drop VP B (dont shake) Let stand 10 min Positive: red/pink Kohns charcoal gelatin o Enzyme: gelatinase

o Positive: diffusion of black pigment o Negative: solid black clumps Sugars/Alcohols o Fermentation o Positive: yellow o Negative: blue/blue-green Nitrate (use the glucose well/tube) o Add reagent A and reagent B o Record nitrite & NO2 result

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