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Gavin Cleveland College Biology 1 Lab Paper 11/7/2013 impalas intramuscularly, they also on separate days, injected the impalas, directly through their jugular veins, while they withdrew the same volume of blood in the syringes, too measure the results. Over the course of the 3 months of experiments, each impala was immobilized 3 to 4 times with sedatives, with a time span of at least 72 hours between each immobilization. After the impalas were immobilized, they would go into the data recording stage, where the scientist would give each impala a score based on how much movement they were doing. Etorphine, was used to sedate the impalas, and once they were sedated, they would injection water into the impalas to increase their oxygen saturation. Doxapram, was used after the injection of etorphine, and had a far greater oxygen increase in the impala, which proved to be less damaging on any tissues. Metoclopramide an buspirone, were used in combination to sedative the impalas as well. Metoclopramide was injected first and after the imobolization was intiated, the impala was then injected with buspirone, which increased the oxygenation of the impalas significantly, by the increase of respiration within 3 minutes of injection. Out of all of the drugs used in these experiments, Buspirone proved to be the least harmful drug used in sedating these impalas. Buspirone, showed a significant increase in oxygenation of the impalas, within minutes after the injection. The use of buspirone, after metoclopramide was the most successful method of sedating an animal, while maintaining minimal oxygenation loss throughout the impalas.
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Article:http://www.jwildlifedis.org/content/46/2/514.full.pdf By:Leith C. R. Meyer,1,2 Robyn S. Hetem,1 Linda G. Fick,1 Duncan Mitchell,1 and Andrea Fuller1 1 Brain Function Research Group, School of Physiology, University of the Witwatersrand, 7 York Road, Parktown, 2193, South Africa 2 Corresponding author (email: Leith.Meyer@wits.ac.za)