HYPOTONIC LYSIS BUFFER

10ml

1
2
3
4
5
6
7
8
9

dd. H2O
Tris (PH7.5)
NaCl
MgCl2
Na3VO4
DTT
EDTA
TLA (-20C)
PMSF (-20C)

Stock Conc.

Total Vol.
Final Conc.

1M
5M
1 M
0.1 M
1M
0.5 M
100X
200 mM

10 mM
10 mM
3 mM
1 mM
1 mM
1 mM
1X
1 mM

750
ul
717.8
7.5
1.5
2.3
7.5
0.8
1.5
7.5
3.8

1000
ul
957
10
2
3
10
1
2
10
5

2000
ul
1914
20
4
6
20
2
4
20
10

3000
ul
2871
30
6
9
30
3
6
30
15

4000
ul
3828
40
8
12
40
4
8
40
20

5000
ul
4785
50
10
15
50
5
10
50
25

6000
ul
5742
60
12
18
60
6
12
60
30

7000
ul
6699
70
14
21
70
7
14
70
35

8000
ul
7656
80
16
24
80
8
16
80
40

10000
ul
9570
100
20
30
100
10
20
100
50

750
ul
680.3
7.5
1.5
2.3
7.5
0.8
1.5
7.5
3.8
37.5

1000
ul
907
10
2
3
10
1
2
10
5
50

2000
ul
1814
20
4
6
20
2
4
20
10
100

3000
ul
2721
30
6
9
30
3
6
30
15
150

4000
ul
3628
40
8
12
40
4
8
40
20
200

5000
ul
4535
50
10
15
50
5
10
50
25
250

6000
ul
5442
60
12
18
60
6
12
60
30
300

7000
ul
6349
70
14
21
70
7
14
70
35
350

8000
ul
7256
80
16
24
80
8
16
80
40
400

10000
ul
9070
100
20
30
100
10
20
100
50
500

750
ul
507
15
42
15
8
1
2
8
4
150

1000
ul
676
20
56
20
10
1
2
10
5
200

2000
ul
1352
40
112
40
20
2
4
20
10
400

3000
ul
2028
60
168
60
30
3
6
30
15
600

4000
ul
2704
80
224
80
40
4
8
40
20
800

5000
ul
3380
100
280
100
50
5
10
50
25
1000

6000
ul
4056
120
336
120
60
6
12
60
30
1200

7000
ul
4732
140
392
140
70
7
14
70
35
1400

8000
ul
5408
160
448
160
80
8
16
80
40
1600

10000
ul
6760
200
560
200
100
10
20
100
50
2000

NUCLEAR ISOLATION BUFFER

10ml

1
2
3
4
5
6
7
8
9
10

dd. H2O
Tris (PH7.5)
NaCl
MgCl2
Na3VO4
DTT
EDTA
TLA (-20C)
PMSF (-20C)
NP40

Stock Conc.

Total Vol.
Final Conc.

1M
5M
1 M
0.1 M
1M
0.5 M
100X
200 mM
10%

10 mM
10 mM
3 mM
1 mM
1 mM
1 mM
1X
1 mM
0.5%

NUCLEAR LYSIS BUFFER

10ml

1
2
3
4
5
6
7
8
9
10

dd. H2O
Tris (Ph7.5)
NaCl
NaF
Na3VO4
DTT
EDTA
TLA (-20C)
PMSF (-20C)
Triton X-100

Stock Conc.

Total Vol.
Final Conc.

1M
5M
0.5 M
0.1 M
1M
0.5 M
100X
200 mM
10%

20 mM
280 mM
10 mM
1 mM
1 mM
1 mM
1X
1 mM
2%

200