Sl no

Std
Glucose

D water

B
1
2
3
4
5
Uk

0
0.2
0.4
0.6
0.8
1
1

1
0.8
0.6
0.4
0.2
0
0

Conc of
sucrose
(mg/ml)
0
20
40
60
80
100
?

Vol of
Anthrone
4
4
4
4
4
4
4

Incubation

O.D 620nm
0
0.143
0.305
0.492
0.644
0.759

0
0.183
0.368
0.485
0.631
0.86

Mean OD
0
0.163
0.3365
0.4885
0.6375
0.8095
0

Standard
deviation
1
0.9

y = 0.008x + 0.0042
R = 0.9995

0.8
0.7
O.D at 620nm

0.000
0.028
0.045
0.005
0.009
0.071

Anthrone method - standard curve

0.6
0.5
0.4
0.3
0.2
0.1
0
0

20

40

60
Conc of Glucose mg/ml

80

100

120

Series1
Linear (Series1)

120