Total Vol:

500

ml

Standard
(ug/ml)
0
1
10
30
50

BSA
ug/ml
50
500
500
500

BSA
ul
0
10
10
30
50

lysis buff.
ul
2
2
2
2
2

Sample
ul
2

ddH2O
ul
398

Reagent
ul
100

Total
ul
500

500

ml

Standard
(ug/ml)
0
1
10
30
50

BSA
ug/ml
50
1000
1000
1000

BSA
ul
0
10
5
15
25

lysis buff.
ul
2
2
2
2
2

Sample
ul
2

ddH2O
ul
398

Reagent
ul
100

Total
ul
500

Total Vol:

ddH2O Reagent
ul
ul
398
100
388
100
388
100
368
100
348
100

Total
ul
500
500
500
500
500

ddH2O Reagent
ul
ul
398
100
388
100
393
100
383
100
373
100

Total
ul
500
500
500
500
500

Date:

13/4/12

Back Gr.

0.66

Standard (ug/ml)
0
1
10
50

Ab

BIO-RAD

Ab -Back Gr.
0
0.751
0.091
0.984
0.324
2.117
1.457
0.66

standard curve
slope

0.0285

y-intercept

0.0332

r2

0.9985

Ab

Ab
1.6
1.4
1.2
1
0.8
0.6
0.4
0.2
0

y = 0.0285x + 0.0332
R = 0.9985

1
2
3
4
5
6
7
8
9
10
11
12

Sample Vol.:
Total Vol.:

2
500

ID
WCL-wt
6ra
C - wt
6ra
N-wt
6ra

Ab

10

20

1.174
1.035
1.878
1.897
0.954
1.028

40

50

ug/ml

ul
ul
Ab -Back Gr.
Ab
0.514
0.375
1.218
1.237
0.294
0.368
-0.66
-0.66
-0.66
-0.66
-0.66
-0.66

30

Conc./tube
ug/ml
16.86
11.99
41.56
42.22
9.15
11.74
-24.32
-24.32
-24.32
-24.32
-24.32
-24.32

Used Sample
Vol. (ul)

2
2
2
2
2
2
2
2
2
2
2
2

Total Vol.
ul

500
500
500
500
500
500
500
500
500
500
500
500

Protein Conc.
ug/ul
4.22
3.00
10.39
10.56
2.29
2.94
-6.08
-6.08
-6.08
-6.08
-6.08
-6.08

0.66
0.751
0.984
1.385
2.117
1.174
1.035
1.878
1.897
0.954
1.028

60

Date:

1
2
3
4
5
6
7
8
9
10
11
12
13
14

Sample
ID
WCL-wt
6ra
C - wt
6ra
N-wt
6ra

13/4/12
ul
72
1X
Protein Conc. Total loading 6X loading
ug/ul
Vol. ul
buffer, ul
4.22
72
12
3.00
72
12
10.39
72
12
10.56
72
12
2.29
72
12
2.94
72
12
72
12
72
12
72
12
72
12
72
12
72
12
72
12
72
12

5%
98% 2MP
ul
3.7
3.7
3.7
3.7
3.7
3.7
3.7
3.7
3.7
3.7
3.7
3.7
3.7
3.7

ug
60
Protein Vol.
ul
14.2
20.0
5.8
5.7
26.2
20.4
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lysis buffer
ul
42.1
36.3
50.6
50.6
30.1
35.9
#DIV/0!
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#DIV/0!