In this experiment we tried to look how pH can control the color of indicator. We also looked at the effect of urease from soya beans on urea. For the last experiment we were focus on how substrate's concentrations affect the enzyme's work. In procedure 1, we predicted that acid would change to red, base changed to purple and the neutral would have no change. From procedure 2, the test tube which has both water and enzyme the color will not change, and for the one that have urea and urease the color will change. And the one that we had choose, which is substrate concentration we predicted that the one that have the most concentration can change the color easier than others. Procedure I
Substance
Color
Citric
Red
Sodium bicarbonate
Purple
Distilled water
Blue Result Procedure I Questions
Why is it important to have three reference tubes? To check if the indicator can work with every states, acid, base and neutral.
Can you predict what color of the solution will be if the urease enzyme convert urea to ammonia? Blue or purple because ammonia is base.
Procedure 2
Substance
Color
Urea
Light purple
Urea +Enzyme
Purple
Water +Enzyme
Blue Result Procedure 2 Questions
What are the purposes of procedure 2 ? To check if the enzyme is working or not. If the enzyme works probably, the tube which has both urea and enzyme must change the color to purple.
Why do we need to add an enzyme into a test tube without urea even though we can predict that no enzyme activity will occur ? Because we need to make sure that enzyme is neutral. If this test tube change color then it mean that enzyme is not neutral and urea is not relate with it.
Effect of substrate concentration on urease
Substance
Color
Color (after 10 min)
Smell
5% Urea +Enzyme
Blue
Green
Best
10% Urea +Enzyme
Blue
Green
Normal
20% Urea +Enzyme
Blue
Green
Worst Result 10 minutes Effect of substrate concentration on urease Questions How does substrate concentration affect the urease activity? How do you know? The one which has the high concentration will has high rate, take less time, to change into ammonia because from our experiment the one which has highest concentration has the most smelly.
Why do you think the color of the indicator slowly changes as times passes? Because it take times to make urea react with water and produce ammonia which is base.
If the concentration of the enzyme was to be increased to twice the amount that was used, what do you think will happen to the color change relative to time? Explain possible reasons to support your answer. The color would change faster because the rate of changing urea to ammonia increases but it will faster until some point which is the maximum point that there are not enough urea to change to ammonia.
Conclusion From all the result, we can conclude that both indicator and enzyme work pretty well. We also conclude that enzymes concentration affect the rate of reaction by looking the result that 20% urea has the worst smell when compare with 10% and 5% then we can conclude that 20% has the most number of ammonia then we can conclude that 20% has the highest rate of reaction. So we can conclude that .