1. The overall performance of MFC depends on the cardinal part of the system; anode.

A number of anode materials are currently in research to adjudge the better one in
terms of the startup time, power output and durability.
2. anode modification and fabrication strategies to increase the power output, durability
and compatibility of the anode interface with the inoculated microorganisms.
3. purely dependent upon the turnover rate of microorganisms, rate of substrate
degradation, effectual performance of electrodes and ion exchange membranes and
internal resistance of the system.
4. The observed low power is attributed to the limitations such as tuning the growth, cultivation
and handling of living microorganisms, understanding the exact mechanism on electron
liberation, sensitivity and low coulombic yield
5.