Manual spectroflourometerRF5301PC

Table of Contents
PIN 220-92340-00
Table of Contents
1. Introduction 1-1
2. RF-5301 PC Components 2-1
2.1 Parts Inspection 2-1
2.2 Instrument Description 2-2
RF-5301PC 2.2.1 Front View 2-2
2.2.2 Right Side View 2-3
Personal Fluorescence 2.2.3 Left Side View 2-4
2.2.4 Rear View 2-5
Hardware 2.3 RF-5301 PC Specifications 2-6
2.4 Optical System 2-8
3. Hardware Installation 3-1
Instruction Manual 3.1 Installation Site 3-1
Revision 1.0 3.2 Installing the Xenon Lamp (Power is OFF) 3-2
3.3 Connecting the PC 3-7
3.4 SWitching Power ON 3-7
3.5 Software Installation 3-8
3.6 Instrument Initialization 3-8
3.7 Adjusting the Light Source Position 3-8
3.7.1 Coarse Adjustment 3-8
3.7.2 Fine Adjustment 3-10
3.8 Resetting the Xenon Lamp Illumination Time 3-11
3.9 Adjusting VRO 3-12
3.10 Performance Check 3-14
4. Maintenance and Inspection 4-1
4.1 Precautions in Relocating the Unit 4-1
4.2 Life Time of the Xenon Lamp 4-1
4.3 Xenon Lamp Illumination Time Management 4-1
4.4 Disposing of a Consumed Xenon Lamp 4-1
4.5 Replacing a Fuse 4-2
4.6 Wavelength Accuracy Check 4-3
4.6.1 Wavelength accuracy check for emission monochromator 4-3
4.6.2 Wavelength accuracy check for excitation monochromator 4-4
4.7 Checking SIN Ratio 4-6
5. Troubleshooting 5-1
) 5.1 Errors During Initialization 5-1
§ SI-IIMAOZU
Shimadzu Scientific Instruments, Inc.
5.2
5.3
Hardware Communication Problems
Hardware Error Messages Related to Software Operation
5-2
5-4
5.4 Troubleshooting Hardware 5-5
7102 Riverwood Drive 5.5 Scanning Problems 5-7

Columbia, MD U.S.A. 21046-2502
41 0/381 -1227
RF-5301PC Hardware Manual iii

Table of Contents Table of Contents
List of Figures
Figure 2-1
Front View 2-2
Figure 2-2
Right Side View 2-3
Figure 2-3
Left Side View 2-4
Figure 2-4
Rear View 2-5
) Figure 2-5
Diagram of the RF-5301PC Optical System 2-9
Figure 3-1
External View ofMain Unit with Dimensions in Millimeters 3-2
Figure 3-2
Lamp Compartment Cover with Placement ofScrews 3-3
Figure 3-3
Slide Plate 3-4
Figure 3-4
Turn Screw Counterclockwise to Loosen 3-4
Figure 3-5
Xenon Lamp 3-5
Figure 3-6
Turn Knurled Screw Clockwise to Mount Lamp 3-5
First Edition: April 1995 Figure 3-7

Turn Clockwise to Secure Lamp 3-6
SHlMADZt: SClENTIFlC INSTRUMENTS, INC. (SSr) PROVIDES THIS PuBUCATION "As Is" WITIlOIJr
Figure 3-8
View of Positive Terminal and Knurled Screw 3-6
WARRANTY OF ANy KIND, ErmER EXPRESS OR lMPUED, INCLUDING, BIJr NOT LIMITED To, THE Figure 3-9
Interface Cable Connection 3-7
lMpUED WARRANnES OF MERCHANTABD..lTY OR FITNESS FOR A PARTICULAR PURPOSE. Some states do Figure 3-10
WL Setting Dialog Box 3-9
not allow disclaimer of express or implied warranties in certain transactions, therefore this Figure 3-11
Position of the White Paper Adjacent to the Cell Holder 3-9
statement may not apply to you.

Figure 3-12
Horizontal Light Source Adjustment 3-9
Any technical inaccuracies, omissions, or typographical errors in this publication will be corrected Figure 3-13
Vertical Light Source Adjustment 3-10
as soon as feasible. Changes are periodically made to the information herein and incorporated into Figure 3-14
Instrument Parameters Dialog Box 3-10
new editions of the publication. SSi may make improvements and/or changes in the product(s) Figure 3-15
Lamp Alignment Indicator 3-11
and/or the program(s) described in this publication at any time.

Figure 3-16
Spectrum Parameters Dialog Box 3-12
It is possible that this publication may contain reference to, or information about, SSi products Figure 3-17
Raman Peak with Cross Hair Displayed 3-13
(instruments and programs), programming, or services that are not announced in your country. Figure 3-18
SIN Ratio Check Dialog Box 3-14
Such references or information must not be construed to mean that SSi intends to announce such Figure 4-1
Fuse Location 4-2
products, programming, or services in your country.

Figure 4-2
Fuse Holders 4-2
This document and the software described in it are furnished under license from SSi and may be Figure 4-3
copied only in accordance with the terms of such license. Figure 4-4
Spectrum Screen (Emission) 4-4
Figure 4-5
Address any comments to: Shimadzu Scientific Instruments, Inc., 7102 Riverwood Drive,
Columbia, MD 21046. SSi may use or distribute any of the information which you supply in any
Figure 4-6
Spectrum Screen (Excitation) 4-6
way it believes appropriate without incurring any obligations whatsoever.
The following registered trademarks are used throughout this document:
IBM, PC DOS and PS/2 are registered trademarks of International Business

Machines, Inc. List of Tables
Microsoft and MS-DOS are registered trademarks and Windows is a trademark Table 2-1
List ofParts 2-1
of Microsoft Corporation.
Table 5-1
Initialization Error Messages 5-1
PopUp Scan rM is a trademark of Shimadzu Scientific Instruments, Inc., U.S.A. ) Table 5-2
Hardware Communication Messages 5-2
Table 5-3
Hardware Error Messages 5-3
SpeedBox" is a registered trademark of Shimadzu Scientific Instruments, Inc.,

Table 5-4
Hardware Error Messages Related to Software Operation 5-4
U.S.A.
Table 5-5
Hardware Problems 5-5
Table 5-6
Hardware Errors Related to Scanning 5-7
© Copyright 1995 Shimadzu Scientific Instruments, Inc., U.S.A. Table 5-7

Hardware Errors Related to Fluorescence 5-7
ii RF-5301PC Hardware Manual iv RF-5301PC Hardware Manual

IntrOduction
1. Introduction
Thank you for your purchase of the RF-5301PC Spectrof]uorometer.
The RF-5301 PC allows high sensitivity analysis to be performed based on a

) unique optical system which involves a highly efficient Blazed Holographic
Grating (BHG) as well as a low-noise circuitry that includes a digital filter.
Chapter 2 of this Hardware Manual includes sections on parts inspection,

views of the instrument with parts identification, specifications, and the optical
system. Later chapters contain details on installation and setup of the
instrument, and maintenance. The last chapter offers help on hardware error
messages, hardware troubleshooting, and scanning problems.
To facilitate operation of this instrument, assistance is available from your

Product Specialist at 410/381-1227.
RF-5301PC Hardware Manual 1-1

RF-5301PC Components
2. RF-5301 PC Components
2.1 Parts Inspection

') This instrument is shipped with the following parts and accessories. Upon
receipt, verify that all of the listed items are included in your shipment.
Table 2-1 List of Parts
Type Description PIN ety
RF-5301PC Main unit and standard

206-81603-92 1
accessories (see below)
Part Power cord (100 - 120V) 071-60814-01 1
RS-232 cable 220-92172-00 1
Supplies 150W xenon lamp 200-81500-0 1
Fuse SA (100-120V) 072-01661-23 2
Tools Phillips screwdriver 200-94612 1
l Screwdriver for sensitivity adjustment 200-94613 1
RF-5301 PC Hardware Manual 2-1

RF-5301PC Components RF-5301PC Components
2.2 Instrument Description 2.2.2 Right Side View
2.2.1 Front View
~
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._~oi
~
.,..~~. (1l0)~1~=~/t'=1~1
\...~;=::::: ;>c:::;: ~
flY
• 0.-i
1.
Figure 2-2 Right Side View
Light source horizontal adjustment screw

Use to adjust the horizontal position of the light source.
11
/
0)
/
2. Light source vertical adjustment screw

/
\ Use to adjust the vertical position of the light source.
-! ~~
I~09?CI \ 3. Lamp retaining screw
1 ;D--fi I Use when installing or removing the lamp. The screw is accessed by
inserting the provided screwdriver through the guide tube.
Figure 2-1 Front View 4. Power switch

Use to switch the instrument ON/OFF.
1. Sample compartment
Set the sample to be measured into the cell holder inside the sample 5. Lamp ON/OFF switch
compartment. Keep this switch in the ON position.
2. Front panel 6. Voltage switch 1
Detach the front panel to install various accessories. Switches voltage setting in 2 steps CAC lOOV, 200V), based on the power
voltage. Use at the factory setting of lOOV.
3. Reagent injection port cover
Detach the reagent injection port cover to inject a reagent or make other 7. Voltage switch 2
additions to the sample cell. Switches voltage setting in 4 steps CAC lOOV, 120V, nov, 240V), based
on the power voltage. Use at the factory setting of l20V.
4. Light source compartment cover )
Detach the light source compartment cover to install or remove the xenon 8. Parallel port
lamp. Not used for this instrument.
5. Power indicator lamp
Indicates that the power is ON when lit.
2-2 RF-5301PC Hardware Manual RF-5301PC Hardware Manual 2-3

9. RS-232C port 2.2.4 Rear View

Used for communication with a personal computer.
10. I/O - 1 connector

Connects to an auto sampler.
11. VO - 2 connector
) ') e"" I (2
Connects to a sipper. 3
o 0
a
O~
2.2.3 Left Side View
~
Figure 2-4 Rear View
1. Sensitivity adjustment VRO
~
~
r ~
2.
Use to adjust the detection sensitivity when the negative high voltage (-HV)
control is set to ON. For adjustment, refer to the Hardware Manual,
Hardware Installation, Chapter 3, Adjusting VRO, Section 3.9.
Sensitivity adjustment VR1

ffj~~~~~1fJ
Use to adjust the detection sensitivity when the negative high voltage
u u control is set to OFF. DO NOT adjust this, as it is set to an appropriate
position prior to shipment of the instrument.
Figure 2-3 Left Side View
3. Sensitivity adjustment VR2
1. Analog output terminal
Use to adjust the detection sensitivity when the light source compensation
Outputs a voltage based on the fluorescence intensity (1 VIFS). Connects
is set to ON. DO NOT adjust this, as it is set to an appropriate position
to Chromatopac, etc.
prior to shipment of the instrument.
2. Contact closure terminal
4. Ground terminal
Close this contact to start a measurement.
Connects terminal to a ground wire. When the power outlet does not
incorporate a ground terminal, be sure to ground the instrument via this
terminal.
) )

2.3 RF-5301 PC Specifications 7. Sensitivity selection Two selectable settings, HIGH and
LOW (sensitivity difference: approx. 50
times)
1. Light source 150W xenon lamp

8. I/O One RS-232C port (for communication
2. Light source compartment Ozone self-extinction lamp housing with PC)
3. Monochromators lon-blazed holographic concave grating \

Sipper interface; Autosampler interface;
for both excitation and emission

Analog output interface
monochromator F/2.5
9. Power 100V, 120V, 220V, 240V AC, 50/60 Hz
3.1 Number of lines per mm 1300 lines / mm
10. Ambient temperature range 15 - 35 Q C
3.2 Wavelength scan range 220 - 900nm and O-order light
11. Ambient humidity range 45 - 80% r.h. [Less than 70% when
3.3 Measuring wavelength range 220 - 750nm and O-order light

temperature is 30 Q C or higher]
3.4 Bandwidth 1.5, 3, 5, 10, 15, 20nm (excitation and

12. Dimensions 670mm (26.5") wide
emission monochromators)
530mm (21 ") deep
1.5,3,5, 10, 15, 20nm, 6HH (half height
on excitation monochromator only)

270mm (10.7") high
3.5 Wavelength accuracy ±1.5 nm

13. Weight 43 kg (95 pounds)
3.6 Wavelength scan speed Selectable in 7 steps: Survey, Super,
Fast, Very Fast, Medium, Slow, and
Very Slow (Survey @ 5,500nm / min.)
3.7 Slewing speed Approximately 20,000nm / min.

D
3.8 SIN Ratio The SIN ratio at the Raman peak of
distilled water is 150 or higher under the
following measurement conditions:
Excitation wavelength: 350nm
Emission wavelength: Peak wavelength
of the Raman line
Bandwidths: 5nm for both excitation and
emission
Response: 2 seconds
4. Detector Photomultipliers for both sample
fluorescence measurement and

monitoring [Sample: R 3788-02;
Monitoring: R 212-14]
)
5. Light source compensation method Dynode feedback method by monitored
excitation light
6. Response o- 98% / 0.02, 0.03, 0.1, 0.25, 0.5, 2, 4,

and 8 seconds
2-6 RF-5301 PC Hardware Manual RF-5301 PC Hardware Manual 2-7

2.4 Optical System 17) Photometric photomultiplier R3788-02

18) Collection point position
The optical system of the RF-5301PC is displayed in Figure 2-5. A 150W 19) Entrance slit
xenon lamp (1) is used as the light source, and in the sample compartment, 20) Exit slit
trapped ozone is broken down by the internal heat of the compartment. The
21) Light quantity balancing aperture
bright spot of the xenon lamp is enlarged via the elliptical mirror (2), and after
being collimated into a beam, is focused via a concave mirror (4) on to the I
excitation slit assembly (3) through the entrance slit (19). Part of the beam,
which is then dispersed to a spectrum via the concave diffraction grating (5), is
directed out of the exit slit (20), passes through a collecting lens assembly
(11), and impinges on the sample cell (12).
The concave diffraction grating used in the spectrofluorometer employs a

19
highly efficient ion-blazed holographic grating produced by an original
Shimadzu technology. For light source compensation, a portion of the
excitation light is reflected by a beam splitter quartz plate (6) to a Teflon
reflecting plate (7), and the scattered light reflected by the Teflon plate is
passed through an aperture (21) to balance the light quantity, and is then
directed to Teflon reflecting plate #2 (8). The scattered light from Teflon plate
#2 is reduced by an optical attenuator (9) and directed to a monitor
photomultiplier (10).
The emitted light from the cell is passed through a lens (13), and directed into
the emission monochromator, consisting of the slit assembly (14) and concave
diffraction grating (15). The spectral light is reflected from a convex mirror
D
(16) and directed to the measurement photomultiplier (17).
1) Xenon lamp 150W
2) Si0 2 coated elliptical mirror
3) Excitation slit assembly
4) Concave mirror
5) Concave diffraction grating (Excitation)
6) Beam splitter quartz plate

10
7) Teflon reflecting plate (1)
8) Teflon reflecting plate (2)

Figure 2-5 Diagram of the RF-5301 PC OpticaL System
9) Optical attenuator
10) Monitor photomultiplier R212-14

11) Collecting lens assembly (2-lens assembly)
12) Cell
)
13) Collecting lens
14) Emission slit assembly
15) Concave diffraction grating (emission)
16) Concave mirror
2-8 RF-5301 PC Hardware Manual RF-5301PC Hardware Manual 2-9

Hardware Installation
3. Hardware Installation
3.1 Installation Site

To take full advantage of the RF-530IPC's performance capabilities and to
ensure its operational stability over a long service life, verify that the
installation site selected satisfies the following requirements.
Please note that inferior performance or damage to the instrument caused by

installation at a site which does not satisfy these requirements may void your
warranty even within the warranty period.
Maintain room temperature between 15 - 35°C.
Avoid a location subject to direct sunlight.
Avoid a location subject to strong vibrations or prolonged weak

vibrations.
Avoid a location subject to intense magnetic or electromagnetic fields.
Maintain the humidity between 45 - 80%. (When temperature is greater

than 30°C, humidity should not exceed 70%.)
Avoid a location subject to corrosive gases or organic/inorganic gases

which absorb in the ultraviolet region.
Maintain in a relatively dust-free environment.
The dimensions of the RF-5301PC are 670 mm (26.5") wide x 530 mm (21")
deep x 270 mm (10.7") high. In addition, the operations are conducted from a
PC, requiring an installation surface area of approximately 1500 mm (59")
wide x 800 mm (31.5") deep, depending on the size of the PC.
The weight of the RF-530IPC is 43 kg (95 Ibs.). Verify that the installation
bench is sturdy enough to support at least this weight.

Hardware Installation Hardware Installation
670 ~ When installing or replacing the lamp, disconnect the power cord from
the power receptacle before removing the lamp compartment cover.
,
Immediately after the power is turned OFF, residual electric charges
~
l ~
o 'w.'
~I
a i could cause an electric shock. Therefore, after the power cord is
I:; [] ,
disconnected, wait at least five (5) minutes before removing the lamp
compartment cover.
~ 1IIIIIiiU )
m~~m~~ .J
$ 0
~ @
a!'i]L-
W
WARNING: Since gas is contained at high pressure inside the xenon
lamp, breakage of the lamp would be very dangerous. Be very careful
• .----1 I I ~13
to avoid imparting any shock, vibration or pressure to the lamp which
could cause it to break (explode). (For details, please refer to the
lamp's inspection certification included with the lamp.)
Lamp Installation Procedure

1. Leave the lamp switch in the ON position.
[
T
c
e-
2. Detach the lamp compartment cover by removing the two retaining
screws.
I N
I I~F·.V_O.QECI
l 01
U \.:::I
Figure 3-1 External View of Main Unit with Dimensions in Millimeters
D
Position of Screws
3.2 Installing the Xenon Lamp (Power is OFF)

When installing the xenon lamp, verify that the power is switched OFF to
prevent electrical shocks, and disconnect the power cable from the power
receptacle.
NOTE: When installing a new bulb, DO NOT touch the glass surface
of the lamp. If accidental contact is made, wipe off the lamp with a Figure 3-2 Lamp Compartment Cover with Placement of Screws
moistened cloth using the cleaner provided with the lamp or wi th a soft
cloth moistened with ethyl alcohol. Fingerprints remaining on the 3. Loosen the two sliding plate retaining screws, and slide the plate to
lamp when the lamp is turned on could result in burnout of the lamp. expose the inside of the lamp compartment.
)
CAUTION: When the xenon lamp is turned on, about 30,OOOV are
applied to the positive electrode (labeled +HV). In addition, while the
lamp is turned on, a 90V charge keeps the lamp above IOOce. Therefore,
ascertain that the lamp compartment cover is in place whenever the
instrument is in use.

5. Remove the xenon lamp from the case and remove the knurled screw at
its negative terminal.
Slide Plate
Xenon lamp bulb
~ cJ-; \\eft'{\
V:~\oef(,( i0'f).~
)~ 0
.,,} h.
:)vJ),\~~ ~
~. (}3V"fi""~
5<' n.~
Figure 3-3 Slide Plate
. {\'7 ~ ~'tv-
J-.- ' cJD L..------?-f=IJ'~-----------=---------'
4. Insert the accessory screwdriver into the guide tube through the lamp
mounting screwdriver hole, and loosen the lamp mounting screw by
~C(Jf' \ ~ Figure 3-5 Xenon Lamp

turning it counterclockwise.
~o--'v)O~.'0Insert the negative terminal of the lamp into the lamp compartment with
either the black or red mark in the cemented part of the terminal facing
the lamp mounting screw.
==
== D
@ ==
== CAUTION: If the lamp terminals are reversed when inserting the
==
== lamp, the lamp will be damaged (explode) when the power is switched
== ON.
Figure 3-4 Turn Screw Counterclockwise to Loosen
,
Facing
the
) lamp
mounting
screw
Figure 3-6 Turn Knurled Screw Clockwise to Mount Lamp

7. Insert the accessory screwdriver through the lamp mounting screwdriver 3.3 Connecting the PC
hole, and secure the lamp in position by turning the lamp mounting screw
clockwise. After the instrument is positioned, the RS-232 interface cable is used to
connect the RF-5301PC to the personal computer (PC). Insert the male cable
connector into the connector labeled RS-232C on the right side of the RF
o ===
5301PC, and secure the cable with the screws. Next, connect one of the
= = female connectors to the PC serial port (IBM compatibles normally have two
==
= = serial ports, COM 1 and COM2), and secure the cable with the screws
==
= =
= =
Figure 3-7 Turn Clockwise to Secure Lamp
8. Close the slide plate and tighten the slide plate set screw.
9. With the positive terminal of the cable attached to the positive terminal of Figure 3-9 Interface Cable Connection
the lamp, fix the xenon lamp in the lamp holder by firmly turning the
knurled screw by hand.
3.4 Switching Power ON
CAUTION: Damage to the xenon lamp could result if the positive Power Supply
terminal is pulled by the power cable while the lamp is ON. When The power consumption of the RF-5301PC is about 400V. Operate the RF
tightening the positive terminal with the knurled screw, be sure to 5301PC from a power supply rated at least 400VA. Supply voltage
leave some slack in the positive terminal cable. Tighten the knurled fluctuations are allowable up to ± 10%. Use a voltage regulator to regulate any
screw ONLY by hand. Using tools, such as pliers or wrenches, could voltage fluctuations above ±lO%.
cause damage to the lamp.
Grounding
The power cable of the RF-5301PC consists of three wires, including the
grounding conductor. Plug the power cable into a three-wire AC outlet for
positive grounding. When the available outlet is of the two-wire type, ground
Positive the unit from either the grounding terminal of the power cable or that on the
Terminal Screw rear of the unit.
Plugging the Power Cable

Plug the accessory power cable into the power connector on the rear of the
unit, but not before checking to see that the power switch is off. Ascertain that
) the voltage setting selector is adjusted appropriately.
Xenon Power Switch

Figure 3-8 View of Positive Terminal and Knurled Screw The xenon power switch above the power switch is then turned ON. When all
the preparations are complete, switch on the power (listen for a clicking
10. Attach the lamp compartment cover. sound). The lamp will automatically light. In addition, verify that the power
display lamp on the front of the instrument is on.
When the xenon lamp does not light, or if there is no clicking sound when the
I.;
power is switched on, or when the clicking sound continues without the xenon E~ Wavelength EM Wavelength -1.J ) / ' ,
lamp lighting, immediately switch the power OFF, and refer to
~ ~6
Troubleshooting, Chapter 5 in this Hardware Manual. ItfJ_J.tI I:jj _lI,l
) Intensity: [9219 I
3.5 Software Installation
I . Read" J 1.~eJ I ~cil.l
See the Software Instruction Manual, Installation, Chapter 2.
Figure 3-10 WL Setting Dialog Box
3.6 Instrument Initialization 2. Open the sample compartment door. As a green beam of light is emitted
from the light source toward the cell, place a piece of white paper just to
the right of the cell holder as shown in Figure 3-11.
NOTE: Before turning the power switch ON, open the sample
compartment door and check to see that no packing material is left
inside and that there is no object in the holder that would block light
transmission.
Run the RF-5301PC software and then turn the power switch ON. Upon start \.
'
up, the unit automatically begins an initialization procedure. Each time an
initialization step is successfully completed, the initialization status box
displays OK, then proceeds to the next step. If an error is encountered, the Figure 3-11 Position of the White Paper Adjacent to the Cell Holder
unit will stop the process. In this case, see Troubleshooting, Chapter 5 in this
Hardware Manual. When the initialization procedure is completed, the main 3. Using the Phillips screwdriver, manually adjust the light source
/
screen appears. horizontal direction for maximum brightness.
'/-J \. ~
cB
3.7 Adjusting the Light Source Position
/ ~ 0°
The Raman lines emitted from distilled water can be used to adjust the light Ct-{\ ,--0\l
source position. Light source position adjustment is required when the xenon \ ,,\ "r!'.' <)J p..J
lamp is installed for the first time or when it is replaced. After turning the , (~\. . ., t'0 {
power switch ON, wait at least thirty (30) minutes before adjusting the light \YJC\{-- '-l0
source position. (~ r ..
\) Ct- .J
Figure 3-12 Horizontal Light Source Adjustment
3.7.1 Coarse Adjustment 0\
1. Click on the Go to WL push button at the bottom of the screen and CAUTION: Adjust the horizontal direction first. If the vertical
enter 500nm for the excitation wavelength (green light). Select the Set
push button.
) direction is adjusted first, you may not be able to adjust the beam of
light.
4. Likewise, manually adjust the light source vertical direction using the
adjustment screw.

3-8 RF-5301PC Hardware Manual
2. Using the number in the center of the Lamp Alignment dialog box as a
guide, adjust the light source horizontal direction adjusting screw for the
@ maximum value (see Figure 3-12).
-, ~!m:""ilI!"allll" _
"I) ~~
lamp Time Used: 260 1~;:a~iji1:·;;:;t
) Ir~; .~i(4r. ~J
Figure 3-13 Vertical Light Source Adjustment

11·653
5. Remove the paper inserted in step 2. Set a cell filled with distilled water
into the cell holder and close the sample compartment door.
Figure 3-15 Lamp Alignment 1ndicator

6. Set the excitation wavelength to 350 and the emission wavelength to 397.
Proceed to the Fine Adjustment.

3. Likewise, adjust the light source vertical direction adjusting screw to its
maximum value (see Figure 3-13). This completes the light source
3.7.2 Fine Adjustment position adjustment.
1. Select Instrument... from the Configure menu. When the dialog box
appears (see Figure 3-14), click on the Lamp Align Perform push button
to bring up the Lamp Alignment dialog box (see Figure 3-15).

3.8 Resetting the Xenon Lamp Illumination Time
The length of time (in hours) that the lamp has been used is indicated in the
'SKip Lamp Alignment dialog box (see Figure 3-15). To reset the xenon lamp
fluorometer: @§~] o Off illumination time, upon installation of the lamp for the first time or when
replacing it, perform the following steps:
-.!::!.V Control: @On o Off
E'MT Protect: @On o Off 1. Select Instrument... in the Configure menu to open the Instrument
Parameters dialog box.
Auto Shutle r:
2. Select the Lamp Align Perform push button to display the Lamp
Dark Level Correction:
Alignment dialog box. Click on the Reset button. (These steps are
SIN Ratio Test: unnecessary when continuing from Installing the Xenon Lamp in this
chapter, Section 3.2.)
Lamp Align:
"·r;/'~·'j';'W"." "',-
Instrument Serial Number: 00000000000001
Instrument ROM Version: 0.90
Lamp Time Used: 91

.- / I Z
'·,=0,gK; "1
Figure 3-14 1nstrument Parameters Dialog Box
3 - 10 RF-5301PC Hardware Manual RF-5301 PC Hardware Manual 3 - l'

3.9 Adjusting VRO 4. Place a cell containing distilled water in the cell holder and close the
sample compartment cover.
The sensitivity adjustment is performed using the Raman peak of distilled
water. 5. Press the Go To WL push button, and set the emission wavelength to
450nm. Select the Set push button. Press the Auto Zero push button and
1. Select the Spectrum mode. then the Start push button to initiate the measurement. When the scan is
q ") completed, enter a file name in the File Name dialog box which appears
2. Select Instrument... in the Configure menu, and set -HV Control and on a Spectrum screen similar to that in Figure 3-17.
PMT Protect to ON. Click OK.
110 ••
3. Select Parameters••• in the Configure menu to display the Spectrum file Acquire Mode .configure Manipulate Eresentation Help
Parameters dialog box (see Figure 3-16). Set the parameters as follows
50000. i i i i. i i i
by tabbing through the box:
Spectrum Type: Emission

EX Wavelength: 350
EM Wavelength Range: 350 - 450
Recording Range: 0.000 (Low); 50.00 (High) %;
~
~
Scan Speed: Fast
Sampling Interval: 1.0nm
EX / EM Slit Width: 5nm / 5nm
Sensitivity: High (
Response Time: Auto
Repeat Scan / Auto File: No setting required at this time. 360.0 360.0 396.1390 420.0 4400 450.0
Wavelength (nm)
Spectrum Iype: o Excitation @Emission o Synchronous

EX ~avelength:
1
3SO Inm
Figure 3-17 Raman Peak with Cross Hair Displayed
EM Wavelength Range: -Start
1
3SO fnd 4SO
1
Recording Range: !"ow I 0.000 High Iso.ooo I 6. Press the right mouse button, and select Cross Hair from the Graph
PopUp menu, and from that submenu, select Display. When the cross
SJ;anning Speed: IFast ~ hair used for reading values appears, position it on the Raman peak of
I~
Sampling Interval [nm):
Slit Width [nm):

11.0
E~
EE EM
EE I 7.
-
397nm to read the peak intensity.
When the peak intensity value is, 10±2{, the following adjustment is
Se!!sitivity: @[H.iii~! o Low unnecessary. When it is outside the stated limit, adjust the uppermost
Resl!onse Time (sec): IAuto [!l volume trimmer (VRO) on the back of the instrument (see RF-5301 PC
Components, Chapter 2, Section 2.2.4 of this Hardware Manual).
IBepeatscan..·II,AutoFile... ) 1m. OKs_II cancet!
NOTE: Do not turn any other trimmer (i.e., VR 1, VR2), as they are
Figure 3-16 Spectrum Parameters Dialog Box adjusted appropriately prior to shipment.
3 - 12 RF-5301PC Hardware Manual RF-5301PC Hardware Manual 3 - 13

8. Repeat steps 5 through 7 until the intensity reading satisfies the 5. The amount of noise determined from the time measurement of the
requirement. Raman peak is calculated as the SIN Ratio. An SIN ratio of at least 150 is
considered normal. When an abnormal value is obtained, please refer to
3.10 Performance Check this Hardware Manual, Troubleshooting, Chapter 5.
After installing the instrument and the lamp, and adjusting the light source position,
the performance check procedure is conducted to verify that the instrument is
~ l ) CAUTION: Contaminated or low-purity distilled water would elevate
operating normally. the baseline, increasing noise.
1. When this operation is not performed immediately after the previous

procedures, switch the power ON and light the lamp, and allow the lamp
to stabilize for at least 30 minutes. Otherwise, ascertain that the lamp has
been on for thirty (30) minutes.
2. Select Instrument... from the Configure menu to display the Instrument

Parameters dialog box, and click on the SIN Ratio Perform push button.
The SIN Ratio Check dialog box appears, along with the message "Fill a
cell with distilled water; Set the cell in the holder." as in Figure 3-18.
Place a cell containing distilled water into the cell holder and close the
sample compartment cover. Click OK to automatically begin the check.
" 1lF-5301 PC ~~r~ q~~~~ \t':r-t :'...

file Acquire t.4ode tontigure Manipulate fresentation Help
[,~ J!rint;t:rl
440.0 4500
! ::[ o
oOOJ[
Filla cell with distilled water.
Set the ullin the holder. ksp";$i;'i;Il'tJ
. 0500~1 ~ ~
" 000 ';;00 [Tf'Eilit'''lilfl
000 200.00
T.ne(sec)
<00'"
10m>
Instrument Serial Number: ~O()OOOOOOOOOOl I
Peak Height: [==:=I SIN Ratio: [==:=I
0000[. ! ,,, ..... , d I
3500 3800 3800 '200 «0.0 .son
~,,",:,"~,,....;.: ~n,,~~"":'-,~"""'''''''_. ~,~m,,,,"_. ..,...
Figure 3-18 SIN Ratio Check Dialog Box J),
3. The Raman peak measurement of distilled water is quickly conducted,
and the peak height is displayed. The SIN Ratio measurement requires
approximately ten (10) minutes.
3 - 14 RF-5301 PC Hardware Manual RF-5301PC Hardware Manual 3 - 15

Maintenance & Inspection
4. Maintenance and Inspection
4.1 Precautions in Relocating the Unit

Be sure to remove the xenon lamp from the unit before transporting the RF
5301PC. (Store the lamp in its special protective case.) Since gas is contained
at high pressure inside the xenon lamp, breakage of the lamp would be very
dangerous. Be very careful to avoid imparting any shock, vibration or
pressure to the lamp which could cause it to break (explode).
Also, when installing a new lamp, DO NOT touch the surface of the lamp bulb.
When accidental contact is made, wipe off the lamp with a piece of cloth
moistened with the cleaner provided with the lamp or with ethyl alcohol.
Fingerprints remaining on the lamp when the lamp is turned on could result in
burnout of the lamp.
4.2 Life Time of the Xenon Lamp

The xenon lamp is a consumable part. After accumulation of excessive hours
of use, the lamp will begin to flicker as the emission point fluctuates. In this
condition, an unacceptable level of noise will be evident.
The recommended usage time is 500 hours. Avoid using the lamp beyond
1000 hours of service. On rare occasions, the lamp could break, causing
major damage to the internal components of the instrument or injury to the
operator.
4.3 Xenon Lamp Illumination Time Management

This instrument incorporates a lamp usage monitoring function. To verify the
accumulated hours of illumination of a lamp, select Instrument... from the
Configure menu in the menu bar. Remember to press the Reset key to reset
the time indicator to 0 after replacing the lamp.
4.4 Disposing of a Consumed Xenon Lamp

Care must be taken when discarding a used xenon lamp. Inappropriate
handling can be extremely dangerous, due to the possibility of the lamp
exploding. When disposing of a used lamp, wrap it in thick cloth or similar
material, and break it with a hammer before disposal.
RF-5301 PC Hardware Manual 4-1

4.5 Replacing a Fuse 4.6 Wavelength Accuracy Check
When the fuse burns out, replace it with a new fuse using the following The emission line (435.8nm) for mercury contained in most fluorescent room
procedure. lights can be used to check the accuracy of the monochromator wavelengths.
1. Using the screwdriver provided, pry open the claw on the fuse cover at 4.6.1 Wavelength accuracy check for emission monochromator
the rear of the instrument.
'" ) 1. Select Spectrum from the Acquire mode menu to conduct a spectrum
measurement.
2. Select Instrument.•. in the Configure menu, and set -HV Control and
PMT Protect to OFF. Click OK.
3. Select Parameters•.. in the Configure menu to display the Spectrum

by tabbing through the box:
Spectrum Type: Emission

EX Wavelength: 350
EM Wavelength Range: 400 - 500
Recording Range: -10.000 (Low); 1000.00 (High)
Scan Speed: Medium
Figure 4-1 Fuse Location Sampling Interval: 0.2nm
2. Remove the fuse holders (two identical holders). EX / EM Slit Width: 3.0nm / 3.0nm
Sensitivity: High
3. Replace the used fuses with new ones, and insert the fuse holders into the
Response Time: Auto
fuse compartment. Be sure to match the arrows on the fuse holders and
the cover. Repeat Scan / Auto File: No setting required at this time.
Spectrum Iype: o Excitation @ Emission o Synchronous

EX 't:!'avelength:
1
350 Inm
EM Wavelength Range: )itart 1

400 I £nd 1500 I
Recording Range: bOW
1-10I !:!igh 1100 0 I
,
/ Fuse SJ;anning Speed: IMedium
f!J
~ ;use
Sampling !nterval [nm): 10.2 f!J
,:~HOld" I Slit Width lnm): EX
E:E EM
EE
j) ) Sensitivity: @High o Low
ResQonse Time [sec]: IAuto [3
Figure 4-2 Fuse Holders IBepellt sc.;n... J I Auto file.:. ,I I.·.· .'§[' ,.1 I· .Canee'l > J
Figure 4-3 Spectrum Parameters Dialog Box
RF-5301PC Hardware Manual RF-5301PC Hardware Manual 4-3

4-2
Maintenance & Inspection Maintenance & Inspection
4. Select OK to validate the parameters. The main screen reappears. As the 4.6.2 Wavelength accuracy check for excitation monochromator
parameters are sent to the instrument, the Fluorometer Window on the 1. Insert a piece of white paper (without fluorescent dye) in the sample
Status Bar indicates Fluorometer Setup. The EX and the EM compartment according to Figure 4-5.
wavelengths and the intensity reading are displayed in the Fluorometer
Window when completed.
5. Open the sample compartment door, remove the cell from the sample
)
compartment, and verify that the light from the fluorescent lamp is
I I
directed into the emission monochromator. (Leave the sample
~
compartment door open for the test.)
/Cell
6. Press the Start push button to initiate measurement. When the

measurement is completed, select Radar. A spectrum is obtained similar
to that shown below, depending on the room lighting conditions.
I'll"
50mm
....8ill.-...
hOlder~
I I;; ~-
Eile ~cquire Mode J;.onfigure Manipulate flresentation ttelp I Excitation

+
... .
14mm
/
light
Size of the white paper

White paper
7 L
i:c
D Figure 4-5 Position of White Paper in Sample Compartment
2. Select Instrument... in the Configure menu, and set -HV Control to

OFF. Click OK.
420 0
3. Select Parameters•.• in the Configure menu to display the Spectrum

.
r·~h'· ~:'.'N 7LG?w.. =~ by tabbing through the box:
Figure 4-4 Spectrum Screen (Emission) Spectrum Type: Excitation
6. Select Peak Pick in the Manipulate menu (see the RF-5301PC Software EM Wavelength: o
Instruction Manual, Peak Pick, Section 5.4.5), and in the Peak Pick EX Wavelength Range: 400 - 500
window, verify that the peak is at 435.8 ±1.5nm. Record the peak Recording Range: 0.000 (Low); 1000.0 (High)
wavelength. If the measured wavelength is not 435.8 ±1.5nm, please
Scan Speed: Medium
contact your Service Representative.
Sampling Interval: 0.2nm
Next, the excitation monochromator wavelength accuracy check is conducted. I) )
EX / EM Slit Width: 3.0nm / 3.0nm
Sensitivity: High
Response Time: Auto
Repeat Scan / Auto File: No setting required at this time.
4-4 RF-5301PC Hardware Manual RF-5301 PC Hardware Manual 4-5

Maintenance & Inspection Maintenance & Inspection
7. Select Peak Pick in the Manipulate menu (see the RF-530J PC Software
Instruction Manual, Peak Pick, Section 5.4.5), and in the Peak Pick
Spectrum Iype: @ Excitation o Emission o Synchronous window, verify that the 450.1nm bright line of the xenon lamp is within
EM ~avelength: 10 1 nm the range of ±2.5nm. The cumulative error, which depends on the
I position of the white paper (approximately ±1 nm), will affect the
.\
EX Wavelength Range: Start 1400 .End
1500 1
Recording Range: low I 0.000 I !:!igh 11000.00 1 ') wavelength accuracy of the instrument.
S.!;anning Speed: IMedium
0 I 8. Set a cell filled with distilled water into the sample compartment.
Sampling !nterval [nm):
1°·2 11] Execute the Lamp Align function from Instrument in the Configure menu
and readjust the VR1 so that the Intensity value falls within the range of
Slit Width (nml: E~
E::E EM
E::E 10.000 ±5.
Sensitivity: @High o Low
Resl!onse Time (sec): IAuto
~, I 9. Press the Start push button to initiate measurement. When the
measurement is completed, select Radar. A spectrum is obtained similar
If!epeats.;a~·..:1 I': Auto fii~ ... ,'I I, .o~ "I r':·· C~;'cel"j to that shown in Figure 4-7.
Figure 4-6 Spectrum Parameters Dialog Box This is an easier method for obtaining the wavelength accuracy of the
instrument. An Hg lamp is necessary to obtain the exact wavelength
4. Select OK to validate the parameters. The main screen reappears. As the accuracy. Please contact Shimadzu or its nearest representative for
parameters are sent to the instrument the Fluorometer Window indicates further information.
Fluorometer Setup. The EX and EM wavelengths and the intensity
reading are displayed in the Fluorometer Window when completed.
4.7 Checking SIN Ratio
5. Select the Go to WL push button at the bottom of the screen. Set the
excitation wavelength to 450nm and the emission wavelength to Onm. D The SIN ratio is checked using the Raman peak of distilled water. For details,
refer to the RF-530I PC Hardware Manual, Performance Check, Section 3.10
6. Adjust VR1 so that the Intensity value falls within the range of 100.000
or the RF-5301 PC Software Instruction Manual, Instrument, Section 5.3.5.
200.000.
.HII.IIi <~l:::"'~
file ,;cquire Mode £onfigure Manipulate e,esentation .t!elp
\;
l:
lSO£lOO
/'
'00 000
73787~-i I I I
)
400.0 420,0 4800 500.0
i ¥I"':""'''''m, •.,w.¥··, .·'''~''·'''·'.F
Figure 4-7 Spectrum Screen (Excitation)

Troubleshooting
5. Troubleshooting
5.1 Errors During Initialization

When the power is switched on to the spectrofluorometer, initialization of the
instrument is performed and eight initialization and self-diagnostic procedures
are conducted. An error message is displayed when an abnormality is
encountered during the initialization sequence. Take the appropriate
corrective action for any errors encountered, according to the directions which
follow.
When an error is generated during initialization, acknowledge the message box

and click on the Start button or Read button on the lower right of the screen
to establish communication between the PC and RF-5301PC, enabling
operation of the instrument. As normal operation of the instrument under this
condition cannot be guaranteed, it is recommended that you call your Service
Representati ve.
Table 5-1 Initialization Error Messages
Error Indication Remedial Action
ROM Contact your Service Representative.
RAM Contact your Service Representative.
EE-PROM Contact your Service Representative.
Excitation Slit Contact your Service Representative.
Emission Slit Contact your Service Representative.
Excitation Monochromator Contact your Service Representative.
Emission Monochromator Contact your Service Representative.
Baseline Is sample compartment cover open? When sample

compartment cover is closed and this error occurs,
contact your Service Representative.
RF-530 1PC Hardware Manual 5-1

Troubleshooting Troubleshooting
5.2 Hardware Communication Problems Table 5-3 Hardware Error Messages
When there is a problem in communication between the PC and RF-5301PC, Error Messages Remedial Action
take corrective action as directed in Table 5-2. When communication is not
being conducted normally, OFF is displayed in the Fluorometer Window of the Cannot open COM port! The specified COM port cannot be opened. This
Status Bar. error occurs when another application is using the
COM port or when there is a major Windows
conflict. In addition, this can also occur when a
Table 5-2 Hardware Communication Messages
nonexistent COM port is specified in the PC
Configuration dialog box.
Item To Be Checked Remedial Action
Communications Initialization There maya problem with the PC hardware. Take
Cable Connection Verify that RS-232C cable is properly connected at
Failed! The specified COM appropriate action as directed by your PC
the RS-232C ports of the PC and the RF-5301PC.
port cannot be initialized. documentation.
After verifying connections and tightening the
connector screws, click the Start or Read button on
the Main screen to reestablish communication. Communications time out! Communication cannot be conducted between the
PC and the RF-5301 PC. Verify cable connections
Cannot communicate with The PC and spectrofluorometer cannot
and the COM port setting in the PC Configuration
spectrofluorometer. communicate. Verify the cable connections and the
dialog box.
appropriate COM port setting in the PC
Configuration dialog box. Failure of DCB initialization! The Device Control Block for the specified COM
port cannot be created. This normally occurs when
COM Port Setting Referring to the documentation provided with your
the RFPC.CFG file is corrupted.
PC, verify that the COM port (RS-232C port) is set
up correctly. Select PC Configuration... from the General communications This error is generated when there is an attem pt to
Configure menu of the RF-5301 PC software, and
confirm that the selected COM port radio button D
error! create a file for a directory which does not exist, or
when the disk is either full or abnormal. When a
(button is black) corresponds to the COM port floppy disk is being used, be sure to insert the
which is actually connected. If the appropriate floppy disk fully into the drive.
button is not currently selected, click on the correct
COM port radio button. After completing the setting, Undefined Control The command indicated is not defined for control of
close the PC Configuration dialog box, and click on Command. the spectrofluorometer, and cannot be used. This
the Start or Read button to reestablish error occurs when the RFPC.CFG file is corrupted
communication. After communication is or when there is a poor cable connection, etc.
established, select Save Parameters... in the
Unknown Communications Same as Undefined Control Command.
Configure menu, and save the current settings to
Error!
the RFPC.CFG file.
5-2 RF-5301 PC Hardware Manual RF-5301PC Hardware Manual 5-3

5.3 Hardware Error Messages Related to Software Operation 5.4 Troubleshooting Hardware
Error messages associated with software operation, the cause of such messages If problems arise with the instrument, take correcti ve action as directed in
and appropriate corrective actions are listed in Table 5-4. Table 5-5. If the symptom does not correspond to any of the descriptions in
the table, contact your Service Representati ve.
Table 5-4 Hardware Error Messages Related to Software Operation
Table 5-5 Hardware Problems
Error Message Cause and Remedial Action Symptom Probable Cause Remedial Action
- - ~
Cannot Auto Zero Displayed when the signal level is too high to allow Power is not
AC cable is not
running the Auto Zero function. Set the sensitivity to supplied when Plug the AC cable firmly into the unit inlet
correctly inserted
LOW and narrow the slit to reduce the level of the power switch is and the AC outlet.
into socket.
signal. When analyzing a solid or powder sample, turned on.
use of an appropriate filter is an effective solution.
When the excitation and emission wavelengths are Power fuse blown. Replace the fuse with a new one.
too close to one another, increase the distance
Any other cause. Call your Service Representative.
between them. If the above measures do not
--
resolve the problem, or if Auto Zero cannot be
SIN Ratio does not
performed even when the shutter is closed, the Poor lamp source
meet specified Re-adjust thelight source position.
instrument may not be operating normally. In this positioning.
value.
case, contact your Service Representative.
Wait at least thirty (30) minutes after
Unstable lamp
Cannot turn ON negative When the lamp is not illuminated, the lamp negative turning on the power to allow the lamp to
illumination.
high voltage (-HV). (Lamp voltage cannot be turned ON. reach a stable state.
does not light.)
Old xenon lamp. Replace with new lamp.
EM monochromator has An error occurred in the emission monochromator
slipped. wavelength drive. Contact your Service
--.
Representative.
Signals are not
The lamp is off. See "The xenon lamp does not light."
generated.
EX monochromator has An error occurred in the excitation monochromator
slipped. wavelength drive. Contact your Service Extremely poor
Representative. position adjustment Re-adjust the light source position.
for light source.
Lamp usage time is invalid. The xenon lamp illumination time is incorrect. Try f---
resetting the lamp illumination time to O. If this Shutter closed. Open the shutter.
problem occurs again, contact your Service
Representative. Improper
Set the wavelength and bandwidth
measurement
properly.
The number of hours the Replace the xenon lamp. condition setting.
lamp has been on has Any other cause. Call your Service Representative.
exceeded SOD! For optimum -
instrument performance
replacement of the lamp is
recommended.
5-4 RF-530 1PC Hardware Manual RF-5301PC Hardware Manual 5-5

Table 5-5 Hardware Problems (continued) 5.5 Scanning Problems

1. The scan of data is very noisy (and not within specifications).
Symptom Probable Cause Remedial Action
The xenon lamp The xenon lamp Turn on the xenon lamp switch on the right Table 5-6 Hardware Errors Related to Scanning
does not light. switch is off. side of the instrument.
)
Verify the connection of the high-voltage Items to be checked Remedial Action
cord to the positive terminal of the xenon
Improper wiring lamp. Be sure to unplug the AC cable Refer to the instruction manual and check the
contact. before making this connection to prevent Instrument operation and performance by following the outlined
the xenon lamp from being turned on procedures.
accidentally.
The light source may have failed. Replace when
Leave the lamp OFF for ten (10) minutes Light source
Overheated lamp. necessary.
to let it cool.

2. The relative fluorescence intensity is abnormal.
Table 5-7 Hardware Errors Related to Fluorescence
Items to be checked I Remedial Action
D Auto Zero has been

selected unintentionally.
I Remove sample, replace with blank and select Auto
(The intensity is set with
I Zero again.
the cell in the cell
holder.)
---- - ------~~~{----------
Wavelength setting is
Set the wavelength appropriately.
not appropriate
----- -~--- -~---~- - - --~~-- --
Faulty sample
, Prepare the sample correctly.
preparation
Improper cell being used: The glass cell is opaque in the UV region. Use a
quartz cell.
Light sensitivity of Use Auto Shutter, increase Scan Speed, and

sample decrease Slit Width.
5-6 RF-5301 PC Hardware Manual RF-5301 PC Hardware Manual 5-7

Hardware Manual Index
Index
A R
Accessories 1-1
Room temperature 2-1
Adjusting VRO 2-12
C SIN Ratio Check 2-14, 3-7
Cable Connections 2-7

Scanning Problems 4-7
Self-diagnostic Procedures 4-1
D Specifications 1-6
Dimensions 2-1
F
Troubleshooting Hardware 4-5
Fuse Replacement 3-2
w
G
Wavelength Accuracy Check
Grounding 2-7
Emission monochromator 3-3
Excitation monochromator 3-5
H Weight ofInstrument 2-1
Hardware Communication Trouble 4-2
Hardware Error Messages 4-4

X
Hardware Installation 2-1

Xenon Lamp
Disposal 3-1
I
Illumination Time Management 3-1
Initialization Errors 4-1

Installation 2-2
Installation Site 2-1

Life Time 3-1
Instrument Description 1-2

Power Switch 2-7
Instrument Initialization 2-8

Resetting Illumination Time 2-11
L
Light Source Position Adjustment
Coarse 2-8
Fine 2-10
o
Optical System 1-8
) Parts Inspection 1-1
Performance Check 2-14
Power Cable 2-7
Power Supply 2-7
Precautions in Relocating the Unit 3-1
RF-5301PC Hardware Manual I - 1

Manual spectroflourometerRF5301PC

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Table of Contents

2. RF-5301 PC Components 2-1

2.1 Parts Inspection 2-1

2.2 Instrument Description 2-2

RF-5301PC 2.2.1 Front View 2-2

2.2.2 Right Side View 2-3

Personal Fluorescence 2.2.3 Left Side View 2-4

2.2.4 Rear View 2-5

Hardware 2.3 RF-5301 PC Specifications 2-6

2.4 Optical System 2-8

3. Hardware Installation 3-1

Instruction Manual 3.1 Installation Site 3-1

3.3 Connecting the PC 3-7

3.4 SWitching Power ON 3-7

3.5 Software Installation 3-8

3.6 Instrument Initialization 3-8

3.7 Adjusting the Light Source Position 3-8

3.7.1 Coarse Adjustment 3-8

3.7.2 Fine Adjustment 3-10

3.8 Resetting the Xenon Lamp Illumination Time 3-11

3.9 Adjusting VRO 3-12

3.10 Performance Check 3-14

4. Maintenance and Inspection 4-1

4.1 Precautions in Relocating the Unit 4-1

4.2 Life Time of the Xenon Lamp 4-1

4.3 Xenon Lamp Illumination Time Management 4-1

4.4 Disposing of a Consumed Xenon Lamp 4-1

4.5 Replacing a Fuse 4-2

4.6 Wavelength Accuracy Check 4-3

4.6.1 Wavelength accuracy check for emission monochromator 4-3

4.6.2 Wavelength accuracy check for excitation monochromator 4-4

4.7 Checking SIN Ratio 4-6

) 5.1 Errors During Initialization 5-1

5.4 Troubleshooting Hardware 5-5

7102 Riverwood Drive 5.5 Scanning Problems 5-7

RF-5301PC Hardware Manual iii

First Edition: April 1995 Figure 3-7

statement may not apply to you.

and/or the program(s) described in this publication at any time.

products, programming, or services in your country.

way it believes appropriate without incurring any obligations whatsoever.

The following registered trademarks are used throughout this document:

IBM, PC DOS and PS/2 are registered trademarks of International Business

SpeedBox" is a registered trademark of Shimadzu Scientific Instruments, Inc.,

© Copyright 1995 Shimadzu Scientific Instruments, Inc., U.S.A. Table 5-7

ii RF-5301PC Hardware Manual iv RF-5301PC Hardware Manual

The RF-5301 PC allows high sensitivity analysis to be performed based on a

Chapter 2 of this Hardware Manual includes sections on parts inspection,

To facilitate operation of this instrument, assistance is available from your

RF-5301PC Hardware Manual 1-1

2.1 Parts Inspection

Table 2-1 List of Parts

Type Description PIN ety

RF-5301PC Main unit and standard

Part Power cord (100 - 120V) 071-60814-01 1

RS-232 cable 220-92172-00 1

Supplies 150W xenon lamp 200-81500-0 1

Fuse SA (100-120V) 072-01661-23 2

Tools Phillips screwdriver 200-94612 1

l Screwdriver for sensitivity adjustment 200-94613 1

RF-5301 PC Hardware Manual 2-1

2.2 Instrument Description 2.2.2 Right Side View