Evaluation of the 1-2 Test for Detecting Salmonella in

Swine Feces
Authors: Erdman M.M.1; Harris D.L.2

Source: Journal of Food Protection, 1 March 2003, vol. 66, no. 3, pp. 518-521(4)

Publisher: International Association for Food Protection

< previous article View Table of Contents

Abstract:

The 1-2 Test is a rapid culture test for the detection of motile Salmonella. The aim of
this study was to evaluate the 1- 2 Test for its ability to detect Salmonella in swine feces
following preenrichment and selective enrichment. Pooled pen fecal samples (n = 118)
and pig rectal swabs (n = 51) were cultured for Salmonella by the 1-2 Test, which was
compared with the standard isolation protocol currently used in our laboratory. In
addition, pen fecal samples known to be free of Salmonella were spiked with various
concentrations of Salmonella enterica subsp. enterica serovar Typhimurium and
cultured by both methods to determine the minimum number of organisms needed to
produce a positive result. When naturally contaminated pen feces and rectal swabs were
used, results obtained with the standard culture method were similar to those obtained
with the 1-2 Test. However, the 1-2 Test did outperform the standard culture method
when the spiked feces samples were tested ( 2 = 4.00). The test kit reduced the time and
materials required for the detection of Salmonella in swine feces. The results of this
study indicate that the 1-2 Test is an accurate method for monitoring Salmonella in
swine feces.

Language: Unknown

Document Type: Miscellaneous

Affiliations: 1: Department of Microbiology; Veterinary Microbiology and

Preventive Medicine, Iowa State University, 207 Science 1, Ames, Iowa 50011,
USA 2: Department of Microbiology; Veterinary Diagnostic and Production
Animal Medicine, Iowa State University, 207 Science 1, Ames, Iowa 50011,
USA