Biophotonics

Module 3: Exercises
NPTEL Biophotonics 1
Exercises
What is Kohler illumination? Why is it required and how
is it implemented in a microscope?
What is the typical resolution limit of optical microscopy?
What sets this limit?
What is dark-field imain? When would you use it
instead of !riht-field imain? instead of !riht-field imain?
What is the difference !etween phase contrast and "#$
imain modes? When would you use these?
What are the typical liht sources used for fluorescence
microscopy
"escri!e the construction of a fluorescence filter set and
explain why each of it%s components is needed
&'(E) Biophotonics 2
Exercises
What are quantum dots? What are some of the
ad*antaes and disad*antaes of quantum dots
compared to fluorescence dyes?
+ow is a confocal microscope a!le to et !etter resolution
than a con*entional microscope?
"escri!e some situations where you would use "escri!e some situations where you would use
fluorescence lifetime imain ,-)#M. and -/E(0
What is 1$(? +ow is it a!le to et imae slices in the
axial direction?
What is the rane of forces that an optical twee2er can
measure? &ame some !ioloical phenomena where this
rane of forces would !e rele*ant
&'(E) Biophotonics 3