Antoine Le Gall

Contact
Information

Centre de Biochimie Structurale

29 rue de Navacelles
CNRS UMR 5048 - UM 1 - INSERM
UMR 1054
34090 Montpellier France

Cell: +33 (0)6 17 09 54 75

Fax: +33 (0)1 64 53 31 01
antoine.legall@cbs.cnrs.fr

Web page

antoinelegall.weebly.com

Citizenship

France

Research
Interests

Biophysics, optics, advanced microscopy techniques, protein DNA interaction, chromosome organisation and segregation.

Education
2008-2011 : Ph.D., Laboratoire Charles Fabry de lInstitut dOptique, Palaiseau,
France
2008-2011 : IFSBM, Institut de Formation Superieure BioMedicale, Institut Gustave Roussy, Villejuif (Biomedical courses for non biologists graduate students)
2007-2008 : Master degree in Optoelectronics, Universite Paris Sud, Palaiseau
2006-2008 : Engineering degree in Optics, Institut dOptique Graduate School, Palaiseau
2005-2006 : M.Sc., Physics and optical telecommunications, Universite de Bretagne
Sud, Lorient
2002-2005 : B.S., Physics, Universite de Bretagne Sud, Lorient

Work
Experience

2012-present: Postdoctoral position, Centre de Biochimie Structurale, Montpellier,

France
Area of Study: Drosophila chromatin insulators (in vitro) and chromosome organisation and segregation in bacteria (in vivo) (supervised by Marcelo Nollmann, Emmanuel Margeat)
2008-2011 : Ph.D., Laboratoire Charles Fabry de lInstitut dOptique, Palaiseau,
France
Area of Study: Optical tweezers and fluorescence microscopy to study the mechanisms of protein synthesis (co-directed by Nathalie Westbrook and Alain Aspect)
Collaboration with Koen Visscher from University of Arizona
May-October 2008 : Internship at National Institute of Standards and Technology,
Gaithersburg, United States
Area of Study: DNA transport in polymer nanotubes (supervised by Kristian Helmerson)
2007-2008 : Engineer project at Laboratoire Charles Fabry, Palaiseau
Area of Study: Optical tweezers to study SECIS loop of mRNA (supervised by
Nathalie Westbrook and Karen Perronet)
June-August 2007 : Engineering internship at New Focus, Division Of Bookham,
San Jose, United States
Area of Study: Responsible for the process implementations of sub-assemblies
of a system of 4 fiber coupled lasers in the production floor (supervised by

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Jimmy Liem)
May-August 2006 : Internship at Ecole Nationale Superieure des Telecommunications de Bretagne
Area of Study: Study and realization of diffractive components for tunable materials and applications to holographic recordings of optical filters (supervised by
Raymond Chevallier)
June-July 2005 : Internship at Universidad Politecnica de Cataluna, Terrassa, Spain
Area of Study: calibration of a CCD camera Pantera 1M60 (supervised by
Maria Millan Garcia-Varela)
Skills
Optics
Main field of study, advanced skills in super resolution microscopy (2D-3D PALM,
3D SIM), optical tweezers, Fluorescence Correlation Spectroscopy (FCS, FCCS),
particle tracking.
Biology
During IFSM courses : Cell culture, cell transfection, cytofluorometric analysis,
western blot and cloning a gene of interest into a plasmid (enzymatic digestion,
PCR, ligation, transformation and screening bacteria colonies).
Teaching
2013 : Teaching super resolution praticals at the EMBO workshop held at the
Centre de Biochimie Structurale, Montpellier, France
2009-2011 : Teaching labworks for under graduate students from the Institut
dOptique : Microscopy, afocal systems, photodetection and diffraction
2009-2011 : Co-supervising different under graduate students doing their internship in the lab,
Languages
French: mother tongue
English: fluent (TOEIC test: 905/990, April 2008)
Spanish: intermediate (2 months internship in Terrassa, Spain, 2005)
Computing
Matlab, IGOR, C, Pascal, Prolog, Labview, Solidworks, OSLO, Mathcad.

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Publications

A. Valeri, A. Le Gall, M. Nollmann. New insights into genome organization, Review

in prep, Trends in genetics .
A. Le Gall, A. Valeri, M. Nollmann. Roles of chromatin insulators in the formation
of long-range contacts, Review in revision, Nucleus, Extra View .
M. Marbouty*, A. Le Gall*, D. Cattoni,A. Koh, A. Cournac, J.B. Fiche, J. Mozzinacci, H. Murray, R. Koszul, M. Nollmann. 3D folding mechanisms and function
of higher-order chromatin domains, In revision, Cell. * These authors contributed
equally to this work
V. Gocheva, A. Le Gall, M. Boudvillain, M. Nollmann, E. Margeat. Direct observation of the translocation mechanism of transcription termination factor Rho, In
revision, Nucleic Acids Research
H. Chommy, N. Fiszman, A. Le Gall, D. Dulin, M. Saguy, N. Westbrook, K. Perronet, O. Namy. Translation kinetics of single mammalian ribosomes, Submitted
to RNA
D. Cattoni, A. Le Gall, M. Nollmann Chromosome organization: Original condensins, Current Biology 24, 3 (2014).
M.U. Richly, S. Turkcan, A. Le Gall, J.-B. Masson, N. Westbrook, K. Perronet, A.
Alexandrou Calibrating optical tweezers with Bayesian inference, Optics Express
21, 25 (2013).
J. Vogelmann*, A. Le Gall*, S. Dejardin, F. Allemand, A. Gamot, G. Labesse,
O. Cuvier, N. Negre, M. Cohen-Gonsaud, E. Margeat, M. Nollmann. Chromatin
insulator factors involved in long-range DNA interactions and their role in the
folding of the Drosophila genome, Plos Genetics10, 8 (2014)
* These authors contributed equally to this work
D. Cattoni, S. Thakur, C.Godefroy, A. Le Gall, J. Lai Kee Him, P.E. Millet, P.
Bron, M. Nollmann. Structure and DNA binding properties of the Bacillus subtilis
SpoIIIE DNA translocase revealed by single-molecule and electron microscopies,
Nucleic Acids Research (2013)
A. Le Gall, D. Dulin, Gilles Clavier, Rachel Meallet-Renault, Philippe Bouyer,
Karen Perronet and N. Westbrook. Improved photon yield of a green dye with a
reducing and oxidizing system, ChemPhysChem 12, 9 (2011)
A. Le Gall, K. Perronet, D. Dulin, K. Visscher, and N. Westbrook. Simultaneous
calibration of optical tweezers spring constant and position detector response,
Optics Express 18, 26 (2010).
D. Dulin, A. Le Gall, P. Bouyer, K. Perronet, N. Westbrook, N. Soler, D. Fourmy
and S. Yoshizawa. Towards single-molecule observation of protein synthesis, Revista Cubana de Fisica, Tecnolaser 2009, Cuba (2009)
D. Dulin, A. Le Gall, K. Perronet, N. Soler, D. Fourmy, S. Yoshizawa, P. Bouyer,
and N. Westbrook. Reduced photobleaching of BODIPY-FL, Physics Procedia,
HBSM, Australia (2009)

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Oral
Presentations

A. Le Gall, M. Marbouty, D. Cattoni, A. Koh, A. Cournac, J.B. Fiche, J. Mozzinacci,

H. Murray, R. Koszul, M. Nollmann. The organization and architecture of Bacillus
subtilis chromosome revealed by super-resolution microscopy and HiC. Seminar
at Centre de Biochimie Structurale. France. 2013.
A. Le Gall, D. Cattoni, J. Reche, J.Y. Bouet, M. Nollmann. Architecture and
dynamics of the SopABC partition system of the F-plasmid in E.coli. GRISBi
meeting. France. 2013.
A. Le Gall, D. Dulin, N. Soler, D. Fourmy, S. Yoshizawa, P. Bouyer, N. Westbrook,
K. Perronet, A. Aspect. Following translation kinetic using quantum dot-labeled
ribosomes Life in Focus Meeting. Switzerland . 2011.
A. Le Gall, K. Perronet, N. Fiszman, D. Dulin, K. Visscher, N. Westbrook. Simultaneous calibration of optical tweezers spring constant and position detector
response.
European Network of Excellence Photonics 4 Life Scientific Meeting. France.
2010.
A. Le Gall, K. Perronet, N. Fiszman, K.Visscher, N. Westbrook. Stretching oligonucleotides using optical tweezers. Seminar at IOGS, Palaiseau. France. 2010.
A. Le Gall, D. Dulin, N. Soler, D. Fourmy, S. Yoshizawa, P. Bouyer, N. Westbrook,
K. Perronet, A.Aspect. Following translation kinetic using quantum dot-labeled
ribosomes. EOS Annual Meeting. France. 2010.
A. Le Gall, D. Dulin, N. Soler, D. Fourmy, S. Yoshizawa, P. Bouyer, N. Westbrook,
K. Perronet, A.Aspect. Translation kinetic of immobilized quantum dot-ribosomal
complexes. Journees de lEcole Doctorale Ondes et Matiere. France. 2010.

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Posters

A. Le Gall, M. Marbouty, D. Cattoni, A. Koh, A. Cournac, J.B. Fiche, J. Mozzinacci,

H. Murray, R. Koszul, M. Nollmann. he organization and architecture of Bacillus
subtilis chromosome revealed by super-resolution microscopy and HiC. American
Society for Cellular Biology, ASCB. Philadelphia. 2014.
A. Le Gall, D. Cattoni, J. Reche, J.Y. Bouet, M. Nollmann. Architecture and
dynamics of the SopABC partition system of the F-plasmid in E.coli. 24`eme
congr`es de la Societe Francaise de Biophysique. Guethary. 2014.
A. Le Gall, D. Cattoni, J. Reche, J.Y. Bouet, M. Nollmann. Architecture and
dynamics of the SopABC partition system of the F-plasmid in E.coli. Biophysical
Society, 58th Annual Meeting. USA. 2014.
A. Le Gall, D. Cattoni, J. Reche, J.Y. Bouet, M. Nollmann. Architecture and
dynamics of the SopABC partition system of the F-plasmid in E.coli. Novel
Optical Microscopies for Biomedical Applications. France. 2013.
A. Le Gall, D. Cattoni, J. Reche, J.Y. Bouet, M. Nollmann. Architecture and
dynamics of the SopABC partition system of the F-plasmid in E.col. SMLMS
meeting. Francfurt. 2013.
A. Le Gall, K. Perronet, D. Dulin, A. Villing, P. Bouyer, K. Visscher, N. Westbrook.
Fast and direct calibration of optical tweezers. EOS Annual Meeting. France.
2010.
A. Le Gall, D. Dulin, P. Bouyer, K. Perronet, N. Westbrook, N. Soler, D. Fourmy and
S. Yoshizawa. Deploiement par pince optique dune tige-boucle dARN impliquee
dans la synthese des selenoproteines. ENS Cachan. 2010.
A. Le Gall, D. Dulin, K. Perronet, P. Bouyer, and N. Westbrook. Increased photostability of Bodipy-FL. Linz winter workshop 12th annual meeting. Austria. 2010.
D. Dulin, A. Le Gall, K. Perronet, P. Bouyer, N. Westbrook, N. Soler, D. Fourmy,
and S. Yoshizawa. Suivi de la synthese de proteines en molecule unique par
microscopie de fluorescence. Congres general de la Societe Francaise de Physique.
France. 2009.
D. Dulin, A. Le Gall, P. Bouyer, K. Perronet, N. Westbrook, N. Soler, D. Fourmy
and S. Yoshizawa. Single-molecule fluorescence microscopy study of the ribosome
translation process. Nanosystem Engineering and Biophotonics, ENS Cachan.
France. 2009.
D. Dulin, A. Le Gall, P. Bouyer, K. Perronet, N. Westbrook, N. Soler, D. Fourmy,
and S. Yoshizawa. Detection and manipulation of single biomolecules for the
study of ribosome dynamics. International OSA Network of Students Meeting
(IONS05). 2009.

References

Dr. Marcelo Nollmann

Lab page

Phone: +33 (0)4 67 41 79 12

marcnol@gmail.com

Prof. Nathalie Westbrook

Institut dOptique

Phone: +33 (0)1 64 53 33 41

nathalie.westbrook@institutoptique.fr

Dr. Karen Perronet

Institut dOptique

Phone: +33 (0)1 64 53 33 48

karen.perronet@institutoptique.fr

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