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NAMES:

DATE PERFORMED:
DATE SUBMITTED:

Laboratory Exercise No. 1


Isolation and Identification of Staphylococci
Results:
1. Table of plate culture results:
Name of
organism

Colonial morphology on BAP

Microscopic
morphology
(BAP)

Appearance on MSA

Hemolysis:

Growth (+/ ):

Pigmentation:

Fermentation (+/ ):

Hemolysis:

Growth (+/ ):

Pigmentation:

Fermentation (+/ ):

Hemolysis:

Growth (+/ ):

Pigmentation:

Fermentation (+/ ):

Hemolysis:

Growth (+/ ):

Pigmentation:

Fermentation (+/ ):

Novobiocin Zone
of diameter

S. epidermidis

S. saprophyticus

S. aureus

E. coli

2. Results of Identification tests:


Name of
organism

Coagulase
(+/)

Time required
to clot plasma

Appearance of
plasma after 4 h

S. epidermidis
S. saprophyticus
S. aureus
*Your interpretation of results from MSA and novobiocin plates.

3. Illustration/ Images:
a. BAP
b. MSA
c. Coagulase Test (+/ tubes)
d. Novobiocin disk Test
e. Rapid latex agglutination test

Mannitol*
(+/)

Novobiocin*
(R/S)

Latex
Agglutination
(+/)

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