oo Ke. (Phest INTER-OFFICE MEMORANDUM Subject: Economic Evaluations of Glucose Dote: November 20, 1970 Isomerase Project To: Mr. Claude M. Sawyer, Jr. CONFIDENTIAL No economic evaluations of the various phases of the glucose isomerase project are required at this time. More research information is required on the various cell lysis procedures (Manton-Gaulin and mold lysis) and the enzyme binding data (IRA-904 and Monsanto system). Once this data is obtained, an economic evaluation should be made of each portion of the project and the alternatives. This evaluation could be based on enzyme reuse, flow rates and the engineering scaleup parameters. The recent reports (RDM, 1970, No. 99 and ROR, 1970, No. 46) are a summary of the pilot plant data on sonification and DEAE-cellulose as an enzyme carrier. The sonification procedure is not commercially feasible (Memo: C. G, Pheil to Mr, S, A. Angotti, October 6, 1970) due to the low flow rate through the unit. However, it is still used as a control system to evaluate other lytic systems. The DEAE-cellulose effort was terminated upon depletion of the enzyme activity in the pilot plant colums. This support is held in reserve as an alternate method if no other support is economically feasible. However, the comercial use of this support is dependent on the development of another type of process operation other than a column operation. This support material is also used as a control system to evaluate the binding levels and enzyme stability of the alternate methods. ne Om Se A 4] V aries CGP:bmg Xe: Mr. E. A, Vassallo Mr. S.A. Angotti Dr. E. C. Ritchel Dr. Murray Senkus Dr. C. E. Teague, dr. Dr. PLC. Markunas Dr. AL H. Laurene Mr. MLR. Haxton Dr. H. J. Bluhm 09> eorts