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, 2010, 18 . PARK Y. et al. Measurement of the nonlinear elasticity of red blood cell membranes.
Phys Rev E Stat Nonlin Soft Matter Phys. 2011 May; 83(5 Pt 1):051925. Epub 2011 May 27. . PubMed,
PMID: 21728589.

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RUSSIAN FEDERATION

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RU

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(51) Int. Cl.

G01N 33/49

(2006.01)

FEDERAL SERVICE
FOR INTELLECTUAL PROPERTY

(12) ABSTRACT

OF INVENTION
(72) Inventor(s):
Skorkina Marina Jur'evna (RU),
Fedorova Marina Zotovna (RU),
Zabinjakov Nikita Aleksandrovich (RU),
Sladkova Evgenija Anatol'evna (RU)

(21)(22) Application: 2011109741/15, 15.03.2011

(24) Effective date for property rights:
15.03.2011

(45) Date of publication: 10.11.2012 Bull. 31

delivering the tipless on a separate hollow polymer

microsphere, and after attached to the tipless the
scanned area is induced to cover the cell suspension,
and force curves are recorded with staying attached
to the sample. The presented method is technically
simple, enables eliminating the cell damage during
force spectroscopy, causes no changes of the
properties of the analysed samples, provides
maintaining nativity and form of the blood cells,
enables producing the most reliable results.
EFFECT: method may be used in general and
clinical physiology for blood cell elasticity test.
1 tbl, 1 ex

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(57) Abstract:
FIELD: medicine.
SUBSTANCE: method involves making a
cantilever, scanning tissue cells, producing force
curves, calculating a Young 's modulus. The
cantilever is made of hollow polymer microspheres of
the diametre of 10 mcm attached on a tipless in the
mode of force spectroscopy. The hollow polymer
microspheres are arranged on the opposite section of
the same glass substrate with a live cell suspension;
the substrate is placed in a chamber saturated with
water vapour, and at first force spectroscopy is
performed at the section of the glass substrate
comprising the hollow polymer microspheres with

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(54) METHOD FOR BLOOD CELL ELASTICITY TEST

2 4 6 6 4 0 1

Mail address:
308015, g.Belgorod, ul. Pobedy, 85, BelGU, otdel
intellektual'noj sobstvennosti, N.D. Tsurikovoj

(73) Proprietor(s):
Federal'noe gosudarstvennoe avtonomnoe
obrazovatel'noe uchrezhdenie vysshego
professional'nogo obrazovanija "Belgorodskij
gosudarstvennyj natsional'nyj issledovatel'skij
universitet"(NIU "BelGU") (RU)

R U

Priority:
(22) Date of filing: 15.03.2011

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1. A-Hassan E., Heinz W.F., Antonik M.D et al. Microelastic mapping of living cells by
atomic force microscopy // Biophys. J. - 1998. V.74 (3). - P.1564-1578.
2. // www.ntmdt.ru/page/nanoindent.
3. Benoit M., Gaub H.E. Measuring cell adhesion force with the atomic force microscope at
the molecular level // Cell tissues organs. - 2002. - V.172. - P.174-189.
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