9.

Growth and nutrition of

microorganisms
Growth : increase in cellular constituents which
results in increase of size and cell division.
Binary fission : growth of an individual cell continues
until the cell divides into 2 new cells of equal size. A)
elongation b) DNA replication c) cell division
Definition : generation: interval for the formation of 2
cells from one cell; generation time/doubling time:
time required for the cell population to double.
Growth cycle
 Lag phase, exponential phase, stationary phase,
death phase
 Instantaneous growth rate constant,  and its
relationship with growth rate constant, k.
Measurement of growth

Total cell count

Viable count : only live cells counted which
forms colonies.
Turbidity measurement : spectrophotometer;
measure unscattered light, unit, OD. Microbial
cells scatter light striking them. Microbial cells
in a population are of roughly constant size,
the amount of scattering is  to the
concentration of cells present. More cells,
more turbid.