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Phages are bacterial viruses that can cause lysis of the bacterial cells they infect. Food associated infections can be traced by phage typing. Nuclei acid hybridization: the procedure assumes that if two species are similar or related, a major portion of their nucleic acid sequence will also be similar.
Phages are bacterial viruses that can cause lysis of the bacterial cells they infect. Food associated infections can be traced by phage typing. Nuclei acid hybridization: the procedure assumes that if two species are similar or related, a major portion of their nucleic acid sequence will also be similar.
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Phages are bacterial viruses that can cause lysis of the bacterial cells they infect. Food associated infections can be traced by phage typing. Nuclei acid hybridization: the procedure assumes that if two species are similar or related, a major portion of their nucleic acid sequence will also be similar.
Droits d'auteur :
Attribution Non-Commercial (BY-NC)
Formats disponibles
Téléchargez comme PPT, PDF, TXT ou lisez en ligne sur Scribd
of bacteria Isolation techniques direct inoculation streaking enrichment cultures identification and classification morphological characteristics cultural characteristics biochemical tests carbohydrate dissimilation: sugar fermentation starch hydrolysis citrate utilization MRVP tests proteolytic tests: gelatin hydrolysis test for hydrogen sulfide indole test Fermentation and proteolysis of milk(action on litmus milk) : acid clot stormy clot sweet clot peptonization Miscellaneous tests urease test catalase test coagulase test nitrate reduction oxidase test Rapid identification systems [(eg: API system)(Analytab products Inc.)]: stiff plastic strip containing 20 ind. Microtubes, each has a depression. Each tube contains a dehydrated medium at the bottom. The dried media is reconstituted into aq form when the inoculum of the test org is added. The strip is then incubated results are read by colour change using charts/computer assisted identification serological tests: studies blood serum and immune responses that are evident in serum eg: slide agglutination test: unknown bac drop into several slides; different known antiserum is added to each sample; bac agglutinate when mixed with antibody (positive test) eg: ELISA eg: Western blotting phage typing: determine which phage a bac is susceptible to. Phages are bacterial viruses that can cause lysis of the bacterial cells they infect. Food associated infections can be traced by phage typing. Eg: a)plate covered by bac b) a drop of different phage is placed on the bac. C) If phage infect the bac., the bac will lyse, clearings appear. nuclei acid hybridization: the procedure assumes that if two species are similar or related, a major portion of their nucleic acid sequence will also be similar. The process measures the ability of DNA strands from one org. to hybridize with the DNA strands of another org. eg: southern blotting. numerical taxonomy: a system that uses overall degrees of similarity and large # of characteristics to determine the taxonomic position of an org.; allows org. of unknown affiliation to be identified as members of established taxa; compared by computer.