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9 Photosynthesis
Essential Idea: Photosynthesis uses the energy in sunlight to
produce the chemical energy needed for life
-the light excites (raises to a higher energy level) an electron inside the
chlorophyll and energy is passed on (successive exciting and the falling back
of electron when energy is emitted) until the electron reaches a specific
chlorophyll molecule at the reaction center
(A pigment molecule inside the photosystem absorbs energy so that it
excites an electron to a higher energy level. When the electron relaxes and
drops back down into the lower, more stable energy state, it passes on the
energy to the next pigment molecule. Eventually, the energy gets passed
onto the reaction center.)
-this process is called photoactivation
-The electron then leaves chlorophyll and is passed down an electron
transport chain
Photosystem II:
-Results in the production of ATP and O2
-When the energized electron leaves the reaction center and is passed along
a electron transport chain, energy is released each time the electron is
passed from carrier to carrier
-The energy that is released causes protons to be pumped across the
thylakoid membrane into the fluid space (thylakoid space) inside the
thylakoid
-this creates a proton gradient (build up of protons, causes concentration
imbalance)
-ATP synthase (enzyme that produces ATP) is also located in the thylakoid
membrane
-they allow protons to flow out across the membrane, down the
concentration gradient
-Energy is released as protons move out: this energy is used to synthesize
ATP
-The coupling of electron transport and ATP synthesis via proton
concentration gradient = chemiosmosis
Photosystem I:
-Absorbs photons and passes electrons along carriers to NADP+ in stroma
- NADP+ accepts 2 electrons from the transport chain and one hydrogen ion
from the stroma to form NADPH
-electrons lost is replaced by photosystem II
-The flow of electrons through the thylakoid membrane is limited by the
amount of NADP+ available to ultimately pick up electrons that are excited
and passed through carriers both in photosystem I and II
-the electrons is then passed back along carriers to photosystem I
-As the electrons is passed along, protons are pumped in across the thylakoid
membrane, producing a concentration gradient
-ATP is synthesized as protons flow back out across the membrane via ATP
synthase (down the concentration gradient)
Calvin Cycle:
-Discovered by Melvin Calvin, Andrew Benson at UC Berkeley in 1945-1955
-used a suspension of unicellular algae to study the steps involved in
changing carbon dioxide to carbohydrates
-used radioactive CO2 (with
14
2. Reduction
-Each glycerate 3-phosphate is then reduced to triose phosphate
-2 ATP, 2NADPH, 2H+ are used to provide energy
3. Synthesis of carbohydrates
-2 glycerate 3-phosphate molecules can be combined to make glucose
phosphate
4. Regeneration of RuBP
-Calvin cycle is a cycle because RuBP is regenerated to replace one that was
used up
-Triose phosphate used to make RuBP
-5 molecules of triose phosphate (3C) are used to make 3RuBP (5C) using
ATP
C3 + C3 ----> C6
C6 + C3 ----> C4 + C5 (RuBP)
C4 + C3 ----> C7
C7 + C3 ----> C5 + C5
-for every 6 molecules of triose phosphate, 5 are converted to RuBP (takes
3 turns of the calvin cycle)
-Light Intensity:
-as light intensity increases, rate of photosynthesis increases
-almost directly proportional
-at high light intensity, the rate becomes constant
-the plant is unable to use light at such high intensity, may even cause
damage to the chlorophyll system
-Effect of temperature:
-at low temperatures, all enzymes work slowly
-at high temperatures, RuBP carboxylase does not work effectively
-the rate limiting step is when the carbon dioxide is fixed onto RuBP