Bacterial Expression Vectors - EMBL

3/31/2015
Vector
Bacterial Expression Vectors - EMBL
Map
PromoterSelection Tag
Seq
m ap
seq
m ap
pBAD/His A
seq
m ap
pBAD/His B
seq
m ap
pBAD/His C
seq
m ap
pBAD/MCS
seq
m ap
pBADM-11
seq
m ap
pBADM-20
seq
Host
kan
am p
none
none
DH5a
tet
am p
CStrep
none
BL21
araBAD
am p
N-His EK
araBAD
am p
N-His EK
araBAD
am p
N-His EK
araBAD
am p
none
araBAD
am p
N-His TEV
araBAD
am p
N-TrxA
TEV
N-His
TOP10
LMG194
TOP10
LMG194
TOP10
LMG194
TOP10
LMG194
TOP10
LMG194
TOP10
LMG194
pACYC177 m apseq
pASK75
Protease
cleavage
site
none
pBADM20(+)
m ap
seq
araBAD
am p
N-TrxA
TEV
N-His
TOP10
LMG194
pBADM-30
m ap
seq
araBAD
am p
N-His
TEV
N-GST
TOP10
LMG194
pBADM30(+)
m ap
seq
araBAD
am p
N-His
TEV
N-GST
TOP10
LMG194
pBADM41(+)
m ap
seq
araBAD
am p
N-His
TEV
N-MBP
TOP10
LMG194
NLL-
TOP10
m ap
Origin
Source
Description
cloning
New England
vector with
Biolabs
p15A origin
expression
pBR322 Biom etra
vector
expression
pUC
Invitrogen
vector
expression
pUC
Invitrogen
vector
expression
pUC
Invitrogen
vector
expression
pUC
A. Geerlof
vector
expression
pUC
A. Geerlof
vector
expression
pUC
A. Geerlof
vector
expression
vector;
contains
pUC
A. Geerlof
two STOP
codons in
MCS
expression
pUC
A. Geerlof
vector
expression
vector;
contains
pUC
A. Geerlof
two STOP
codons in
MCS
expression
vector;
contains
pUC
A. Geerlof
two STOP
codons in
MCS
p15A
https://www.embl.de/pepcore/pepcore_services/strains_vectors/vectors/bacterial_expression_vectors/popup_bacterial_expression_vectors/
expression
1/9
3/31/2015
pBADM-52 seq
pBADM52(+)
m ap
seq
m ap
pBADM-60
seq
pBADM60(+)
pBAT4
pBAT5
pCal-n
pET-3a
pET-3b
pET-3c
m ap
seq
m ap
seq
m ap
seq
m ap
seq
m ap
seq
m ap
seq
m ap
seq
araBAD
araBAD
araBAD
am p
DsbA TEV
N-His
LMG194
pUC
A. Geerlof
vector
am p
LLDsbA TEV
N-His
TOP10
LMG194
pUC
A. Geerlof
expression
vector;
contains
two STOP
codons in
MCS
am p
NNusA TEV
N-His
TOP10
LMG194
pUC
A. Geerlof
expression
vector
araBAD
am p
NNusA TEV
N-His
T7
am p
none
none
BL21(DE3)
pUC
M. Hyvnen
T7
am p
none
none
BL21(DE3)
pUC
M. Hyvnen
T7/lac
am p
N-CBP Thr
BL21(DE3)
ColE1
Stratagene
T7
am p
none
none
BL21(DE3)
pBR322 Novagen
T7
am p
none
none
BL21(DE3)
pBR322 Novagen
T7
am p
none
none
BL21(DE3)
pBR322 Novagen
TOP10
LMG194
pUC
A. Geerlof
pET-3d
m ap
seq
T7
am p
none
none
BL21(DE3)
pBR322 Novagen
pET-12a
m ap
seq
T7
am p
leader
none
seq.
BL21(DE3)
pBR322 Novagen
T7
am p
N-His Thr
BL21(DE3)
pBR322 Novagen
T7/lac
am p
N-His Thr
BL21(DE3)
pBR322 Novagen
T7/lac
am p
N-His Xa
BL21(DE3)
pBR322 Novagen
T7/lac
am p
N-His Ent
BL21(DE3)
pBR322 Novagen
T7
am p
leader
seq.
none
C-His
BL21(DE3)
pBR322 Novagen
T7/lac
am p
C-His none
BL21(DE3)
pBR322 Novagen
pET-14b
pET-15b
pET-16b
pET-19b
m ap
seq
m ap
seq
m ap
seq
m ap
seq
m ap
pET-20b(+)
seq
pET-21d(+)
m ap
expression
vector;
contains
two STOP
codons in
MCS
expression
vector
expression
vector
expression
vector
expression
vector
expression
vector
expression
vector
expression
vector
form erly
pET-8c
expression
vector for
secreted
proteins
expression
vector
expression
vector
expression
vector
expression
vector
expression
vector for
secreted
proteins
expression
2/9
3/31/2015
seq
pET-22b(+)
pET-24d(+)
pET-28a
pET-28c
m ap
seq
m ap
seq
m ap
seq
m ap
seq
T7/lac
am p
leader
seq.
none
C-His
BL21(DE3)
pBR322 Novagen
T7/lac
kan
C-His none
BL21(DE3)
pBR322 Novagen
T7/lac
kan
BL21(DE3)
pBR322 Novagen
T7/lac
kan
BL21(DE3)
pBR322 Novagen
N-His
Thr
C-His
N-His
Thr
C-His
pET-32a(+)
m ap
seq
T7/lac
am p
N-Trx
Thr
N-His
EK
C-His
AD494(DE3)
Origam i
pBR322 Novagen
(DE3)
pET-32b(+)
m ap
seq
T7/lac
am p
N-Trx
Thr
N-His
EK
C-His
AD494(DE3)
pBR322 Novagen
Origam i(DE3)
pET-32c(+)
m ap
seq
T7/lac
am p
N-Trx
Thr
N-His
EK
C-His
AD494(DE3)
pBR322 Novagen
Origam i(DE3)
pET-39b(+)
m ap
seq
T7/lac
kan
m ap
pET-40b(+)
seq
T7/lac
kan
NDsbA
C-His
NDsbC
N-His
C-His
Thr
EK
Thr
EK
BL21(DE3)
BL21(DE3)
pBR322 Novagen
pBR322 Novagen
pETM-10
m ap
seq
T7/lac
kan
H-His
none
C-His
BL21(DE3)
pBR322 G. Stier
pETM-11
m ap
seq
T7/lac
kan
N-His TEV
BL21(DE3)
pBR322 G. Stier
kan
N-His
NSUMO
SUMO3
BL21(DE3)
pBR322 H. Besir
pETM11m ap
SUMO3GFP seq
T7/lac
vector
expression
vector for
secreted
proteins
expression
vector
expression
vector
expression
vector
expression
vector for
disulfide
bond
containing
proteins
expression
vector for
disulfide
bond
containing
proteins
expression
vector for
disulfide
bond
containing
proteins
vector for
expression
in periplasm
vector for
expression
in periplasm
contains
EFH insert
in m odified
pET-24d
contains
MAD insert
in m odified
pET-24d
pETM11
with E. coli
optim ized
synthetic
sum o3 gene
contains
3/9
3/31/2015
pETM-12
m ap
seq
T7/lac
kan
N-His Thr
BL21(DE3)
pBR322 G. Stier
pETM-13
m ap
seq
T7/lac
kan
none
BL21(DE3)
pBR322
pETM-14
m ap
seq
T7/lac
kan
N-His 3C
BL21(DE3)
pBR322
pETM14_ccdB
m ap
seq
T7/lac
kan
N-His 3C
BL21(DE3)
pBR322
pETM-20
m ap
seq
T7/lac
am p
N-Trx
TEV
N-His
BL21(DE3)
pBR322
pETM-21
m ap
seq
T7/lac
am p
N-Trx
Thr
N-His
BL21(DE3)
pBR322
pETM-22
m ap
seq
T7/lac
kan
N-Trx
3C
N-His
BL21(DE3)
pBR322
pETM22_ccdB
m ap
seq
T7/lac
kan
N-Trx
3C
N-His
BL21(DE3)
pBR322
pETM-30
m ap
seq
T7/lac
kan
N-His
TEV
N-GST
BL21(DE3)
pBR322
pETM-33
m ap
seq
T7/lac
kan
N-His
3C
N-GST
BL21(DE3)
pBR322
pETM33_ccdB
m ap
seq
T7/lac
kan
N-His
3C
N-GST
BL21(DE3)
pBR322
pETM-40
m ap
seq
T7/lac
kan
none
N-MBP TEV
BL21(DE3)
ABD insert
in m odified
pET-9d
contains
ABD insert
G. Stier
in m odified
pET-24d
m odified
pET-24d;
contains
A. Geerlof
two STOP
codons in
MCS
S. Suppm ann pETM-14
(MPI
m odified for
Biochem istry) SLIC
contains
RAGE insert
G. Stier
in m odified
pET-32
contains
RAGE insert
G. Stier
in m odified
pET-32
m odified
pET-24d;
contains
A. Geerlof
two STOP
codons in
MCS
(MPI
m odified for
Biochem istry) SLIC
contains
DCOH
G. Stier
insert in
m odified
pET-24d
m odified
pET-24d;
contains
A. Geerlof
two STOP
codons in
MCS
(MPI
m odified for
Biochem istry) SLIC
pBR322 G. Stier
m odified
pET-24d;
contains
two STOP
4/9
3/31/2015
pETM-41
m ap
seq
T7
kan
N-His
TEV
N-MBP
BL21(DE3)
pBR322
pETM-43
m ap
seq
T7/lac
kan
N-MBP 3C
BL21(DE3)
pBR322
pETM-44
m ap
seq
T7/lac
kan
N-His
3C
N-MBP
BL21(DE3)
pBR322
pETM44_ccdB
m ap
seq
T7/lac
kan
N-His
3C
N-MBP
BL21(DE3)
pBR322
pETM-50
m ap
seq
T7/lac
kan
NDsbA TEV
N-His
BL21(DE3)
pBR322
pETM-51
m ap
seq
T7/lac
kan
NDsbA Thr
N-His
BL21(DE3)
pBR322
pETM-52
m ap
seq
T7/lac
kan
LLDsbA TEV
N-His
BL21(DE3)
pBR322
codons in
MCS
m odified
pET-9d;
contains
G. Stier
two STOP
codons in
MCS
m odified
pET-24d;
contains
A. Geerlof
two STOP
codons in
MCS
m odified
pET-24d;
contains
A. Geerlof
two STOP
codons in
MCS
(MPI
m odified for
Biochem istry) SLIC
contains
RAGE insert
in m odified
pET-24d;
G. Stier
leader
sequence for
export to
periplasm
contains
RAGE insert
in m odified
pET-24d;
G. Stier
leader
sequence for
export to
periplasm
contains
RAGE insert
in m odified
pET-24d;
G. Stier
leaderless
(LL) DsbA
fusion for
cytoplasm ic
expression
m odified
pET-24d;
leader
sequence for
5/9
3/31/2015
pETM-54
m ap
seq
T7/lac
kan
N3C
DsbA
N-His
BL21(DE3)
pBR322 A. Geerlof
kan
LLDsbA 3C
N-His
BL21(DE3)
pBR322 A. Geerlof
T7/lac
kan
NNusA TEV
N-His
BL21(DE3)
pBR322 G. Stier
m ap
seq
T7/lac
kan
NNusA 3C
N-His
BL21(DE3)
pBR322 A. Geerlof
pETM-70
m ap
seq
T7/lac
kan
N-CBP TEV
BL21(DE3)
pBR322 A. Geerlof
pETM-80
m ap
seq
T7/lac
kan
NDsbC TEV
N-His
BL21(DE3)
pBR322 G. Stier
pETM-82
m ap
seq
T7
kan
LLDsbC TEV
N-His
BL21(DE3)
pBR322 G. Stier
pGAT
m ap
seq
T7
am p
N-His
Thr
N-GST
BL21(DE3)
pUC
M. Hyvnen
T7
am p
N-His
Thr
N-GST
BL21(DE3)
pUC
M. Hyvnen
tac
am p
N-GST Xa
BL21
pBR322 Pharm acia
tac
am p
N-GST Thr
BL21
pBR322 Pharm acia
pETM-55
m ap
seq
pETM-60
m ap
seq
pETM-66
m ap
seq
m ap
pGEX-3X
seq
m ap
pGEX-4T-1
seq
m ap
pGAT2
T7/lac
export to
periplasm ;
contains
two STOP
codons in
MCS
m odified
pET-24d;
leaderless
(LL) DsbA
fusion for
cytoplasm ic
expression;
contains
two STOP
codons in
MCS
contains
ABD insert
in m odified
pET-24d
m odified
pET-24d;
contains
two STOP
codons in
MCS
m odified
pET-24d
m odified
pET-24d;
leader
sequence for
export to
periplasm
m odified
pET-9d;
leaderless
(LL) DsbC
fusion for
cytoplasm ic
expression
expression
vector
expression
vector
expression
vector
expression
vector
expression
6/9
3/31/2015
pGEX-4T-2 seq
pGEX-4T-3
pGEX-5X-1
pGEX-5X-2
pGEX-5X-3
pGEX-6P-1
pGEX-6P-2
pGEX-6P-3
pHAT
pHAT2
m ap
seq
m ap
seq
m ap
seq
m ap
seq
m ap
seq
m ap
seq
m ap
seq
m ap
seq
m ap
seq
tac
am p
N-GST Thr
BL21
pBR322 Pharm acia
tac
am p
N-GST Thr
BL21
pBR322 Pharm acia
tac
am p
N-GST Xa
BL21
pBR322 Pharm acia
tac
am p
N-GST Xa
BL21
pBR322 Pharm acia
tac
am p
N-GST Xa
BL21
pBR322 Pharm acia
tac
am p
N-GST Pre
BL21
pBR322 Pharm acia
tac
am p
N-GST Pre
BL21
pBR322 Pharm acia
tac
am p
N-GST Pre
BL21
pBR322 Pharm acia
T7lac
am p
N-His none
BL21(DE3)
pUC
M. Hyvnen
T7lac
am p
N-His none
BL21(DE3)
pUC
M. Hyvnen
lacIq
containing
strains eg.
JM105
pBR322 Pharm acia
expression
vector
Pharm acia
expression
vector
pKK223-3
m ap
seq
tac
am p
none
none
pKK223-2
m ap
seq
trc
am p
none
none
pMal-c2
m ap
seq
pMal-p2
m ap
seq
m ap
seq
m ap
pProEx HTb
seq
m ap
pProEx HTc
seq
pProEx HTa
tac
TB1 or
any lacZa
com plem enting
strain
TB1 or
any lacZa
com plem enting
strain
am p
N-MBP Xa
tac
am p
leader
seq.
Xa
N-MBP
trc
am p
N-His TEV
BL21
trc
am p
N-His TEV
BL21
trc
am p
N-His TEV
BL21
T5/lac
am p
NDHFR none
C-His
M15[pREP4];
SG13009
ColE1
[pREP4]
pQE-16
m ap
seq
pQE-30
m ap
seq
T5/lac
am p
N-His none
pQE-31
m ap
seq
T5/lac
am p
N-His none
pBR322
vector
expression
vector
expression
vector
expression
vector
expression
vector
expression
vector
expression
vector
expression
vector
expression
vector
expression
vector
New England expression

Biolabs
vector
vector for
New England
pBR322
expression
Biolabs
in periplasm
M15[pREP4];
SG13009
ColE1
[pREP4]
M15[pREP4];
SG13009
ColE1
[pREP4]
Life
Technologies
Life
Technologies
Life
Technologies
expression
vector
expression
vector
expression
vector
Qiagen
expression
vector
Qiagen
expression
vector
Qiagen
expression
vector
7/9
3/31/2015
pQE-32
m ap
seq
T5/lac
am p
N-His none
pQE-60
m ap
seq
T5/lac
am p
C-His none
pQE-70
m ap
seq
T5/lac
am p
C-His none
M15[pREP4];
ColE1
SG13009
[pREP4]
M15[pREP4];
SG13009
ColE1
[pREP4]
M15[pREP4];
SG13009
ColE1
[pREP4]
Qiagen
expression
vector
Qiagen
expression
vector
Qiagen
expression
vector
pQE-80L
m ap
seq
T5/lac
am p
N-His none
M15
ColE1
Qiagen
pQE-81L
m ap
seq
T5/lac
am p
N-His none
M15
ColE1
Qiagen
pQE-82L
m ap
seq
T5/lac
am p
N-His none
M15
ColE1
Qiagen
T7
am p
N-His EK
ColE1
Invitrogen
T7
am p
N-His EK
ColE1
Invitrogen
T7
am p
N-His EK
ColE1
Invitrogen
trc/lac
am p
C-His none
BL21
pUC
Invitrogen
trc/lac
am p
C-His none
BL21
pUC
Invitrogen
trc/lac
am p
C-His none
BL21
pUC
Invitrogen
trc/lac
am p
C-His none
BL21
pUC
Invitrogen
pRSET A
pRSET B
pRSET C
pTrcHis2 A
pTrcHis2 B
pTrcHis2 C
pTrcHis2/
LacZ
m ap
seq
m ap
seq
m ap
seq
m ap
seq
m ap
seq
m ap
seq
m ap
seq
BL21(DE3)pLysS
BL21(DE3)pLysS
BL21(DE3)pLysS
pZA31-Luc
m ap
seq
PLtetO-1 cam
none
none
DH5aZ1
p15A
H. Bujard
pZE12-Luc
m ap
seq
PLlacO-1 am p
none
none
DH5aZ1
ColE1
H. Bujard
pZE21MCS-1
m ap
seq
PLtetO-1 kan
none
none
DH5aZ1
ColE1
H. Bujard
pZS*24MCS-1
m ap
seq
Plac/ara-1 kan
none
none
DH5aZ1
pSC*101 H. Bujard
expression
vector also
expressing
lac I
expression
vector also
expressing
lac I
expression
vector also
expressing
lac I
expression
vector
expression
vector
expression
vector
expression
vector
expression
vector
expression
vector
expression
vector
contains
luciferase
gene
contains
luciferase
gene
high copy
num ber
expression
vector
low copy
num ber
expression
vector
8/9
3/31/2015
9/9

Bacterial Expression Vectors - EMBL

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Bacterial Expression Vectors - EMBL

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3/31/2015