a RoutNe, [] ] TEST BOOKLET No, f 27 TEST FOR POST GRADUATE PROGRAMMES BIOTECHNOLOGY Time: 2 Hours Maximum Marks: 450 INSTRUCTIONS TO CANDIDATES 1. Yow are provided with a Test Booklet and an Optical Mark Reader (OMR) Answer Sheet to mark Aras Bases Po not soil the Answer Sheet. Read carefully all the instructions piven om the ‘Answer Sheet, 2 Write your Rell Number inthe space provided on the top of this page. > Also write your Roll Number, Test Code, and Test Subject in the colurans provided forthe same ‘on the Answer Sheet, Darken the appropriate bubbles with a Ball Point Pen, ‘4. The paper consists of 150 objective type questions. All questions carry equal marks 5 Bach question has four alternative responses marked A, B, C and D and you have to darken the bubble fully by a Ball Point Pen comesponding tothe correct response as indice inthe example shown on the Answer Sheet. & Each correct answer caries 3 marks and each wrong answer caries 1 minus mark. 7- Please do your rough work only onthe space provided fori atthe end ofthis Test Booklet 5 Mou should retum the Answer Sheet to the Invgiltor before you leave the examination ball However, you can retain the Test Booklet, °- Every precaution has been taken to avoid eros in the Test Booklet. In the event of such Seoreseen happenings the same may be brought to the notice of the ObsewerChar Superintendent in writing. Suitable remedial measures will be taken at the time of evaluation, if necessary, SEALCorIz 1 BIOTECHNOLOGY Which of the following transgenic plant is a B-carotene yielder? (A) Rice (B) Carrot (C) Pea (D) Tomato LSC and SSC regions of the genome are found in (A) nucleus (B)_ mitochondrion (©) chloroplast (D) bacterium The CCMB is situated in (A) Lucknow (B) New Delhi (C) Hyderabad (D) Chandigarh ‘Ac-Ds higher plant transposon was first described in (A) maize (B)_arabidopsis, {C)_ tobacco (D) pea A bacterium can divide every 20 min, Beginning with a single individual, how many bacteria will there be in the population, if there is an exponential growth for 3 hours? (A) 18 (B) 440 (© 512 (D) 1024 Anallergic reaction is initiated by the following antibody (A) IgG (B) IgM (C) IgA (D) IgE DNA foot-printing is used to (A) settle disputed parentage (B) detect protein-binding site on the DNA. (©) clone DNA (D) separate DNA by PFGE8 10. WL 12. 13. 14, 15. 0112 Which one of the following genes confers freezing tolerance in animals? (A) Aaa3 (B) CAT (©) Aroa (D) ADH Producing a blue-coloured rose involves manipulation of (A) flavonoid biosynthesis pathway (B) carotenoid biosynthesis pathway (©) chlorophyil biosynthesis pathway (D) alkaloid biosynthesis pathway Glyphosate is a herbicide which inhibits (A)_ pigment biosynthesis (B) nucleic acid biosynthesis (C)_ energy production (D) aromatic amino acid biosynthesis, In polymerase chain reaction, the DNA denaturation temperature is (a) 95°C (B) 75°C (©) 120°C (D) 65°C P53 is implicated in (A) cystic fibrosis (B) tumor suppression (C)_alzheimers (D) AIDS ‘The most abundant polysaccharide on Earth is (A) cellulose (B) chitin (©) lignin (D) starch ‘The most abundant RNA present in the cell is (A) mRNA (B) tRNA (© RNA (D) anti-sense RNA GFP is isolated from (A) chloroplast (B) flowers (©)_jelly fish (D) wings of butterfly(NO A 16. 17. 18. 19. 20. 2 2. 23. Cloning was first carried out in (A) monkey (B) cat (C)_ guinea pig (D) lamb Guy's 13 Ab has been successfully generated through (A). stem cell culture (B) hela cell culture (©) tobacco root culture (D) hybridoma cell culture “Thousands of gene expression can be studied using the following technique: (A) DNA finger printing (B) PCR (C)_ Gene finder (D) Micro array technique “Which one of the following is involved in alcohol production? (A) Aspergillus (B) Zygomonas (©) Bacillus {D) Acetobacter By which method can DNA be directly introduced into a cell without a vector? (A) Gene knock out (B)_ Gene amplification (C)_ Antisense RNA technology (D) Electroporation Dideoxy nucleotide is commonly employed in (A) mutation (B) nucleotide substitution (C)_ DNA sequencing (D) DNA repair Which of the following enzyme is specifically used in PCR? (A) Ligase (B) Reverse transcriptase (C)_ Methylase (D) Tag polymerase PCR was invented by (A) EM Souther (B) Kerry Mullis (©) Melvin Calvin (D) Hatch and Slack‘OES A 60112} 26. 27, 28, 29, 30. 31 The Hybridoma cells have the capacity of producing, (A) RBC (B) antigen (C) CD4 cells (D) antibodies RFLP can be used in (A) forensic study (B) intron analysis (C) amplification of template (D) comparison of closely related species Chloroplast contains the following pigments: (A). Chlorophyll a (B)_ Chlorophyll b (C) Carotenoids (D) Allof the above Which of the following gas is involved in global warming? (A) co (B) CH, (C) SO. (D) Os The cultivation of honey bees is called (A) agriculture (B) sericutture (©) apiculture (D) moriculture Cells without cell wall are called (A) tonoplasts (B) protoplasts (C) leucoplasts (D) chromoplasts Virus-fiee plants can be obtained through (A) root culture (B)_ mericloning (C) callus culture (D) floricutture An organism capable of causing disease is known as (A) phage (B)_ mycoplasma (©) fungus (D) pathogen{ANE (az) 32, 33 34, 35, 36, 37, 38, 39. 5 Which one of the following plant is used for bio-diesel production? (A) Sunflower (B) Castor (C) Jatropha (D) Groundnut The haploids can be raised from (A). endosperm (B) callus (C)_ somaclone (D) pollen The structure of the protein is mostly determined by (A) hydrogen bonds (B) amino acid sequence (C)_ disulphide bridges (D) polypeptides ‘The number of units of activity per mg protein of enzyme is known as (A) specific activity (B) tum over number (©) molar activity (D) allosteric activity Crown-gall is caused by (A) candida (B) fusarium (©) agrobacterium (D)azospirillum Incommercially important seeds, the dormancy can be broken by (A) 2,4-D (B) ABA (© GAs (D) BA Which among the following is an important rice biofertilizer? (A) Water hyacinth (B) Ottelia (©) Pistia (D) Azolla ‘Non Watson-Crick pairing is most common in (A) A-DNA (B) B-DNA (© CDNA (D) Z-DNA40. 4l 42, 43. 44, 45. 46. 41. olla 6 The first transgenic animal reported was a (A) mouse (B) pig (C) fish (D) lamb Which of the following is a post-translational event? (A) Addition of polyA tail (B)_ Methylation (©) Demethylation (D)_ Glycosylation Which of the following antibiotics is of recent origin? (A) Penicillin (B) Tetracyclin (C) Streptomycin (D) Ciprofloxacin Which of the following environmentally friendly marker is employed in modem molecular biology? (A) Antibiotics (B) Tracers (C)_ EPSPS (D) Metabolic genes Cells have a mechanism that removes introns and yields mRNA. This process is specifically called as (A)_ RNA splicing (B)_ RNA maturation (C)_ processing of RNA (D) RNA editing Among the following, which one of them is not protein but an enzyme? (A) Metallo enzyme (B) Allosteric enzyme (C) Zymogen (D) Ribozyme The process of making a product of a gene is (A) genetic map (B) gene therapy (©) gene expression (D) genetic engineering An RNA molecule that complements another RNA is called (A) mRNA (B)_ RNA duplex (C)_ amplification (D) antisense RNAA CUA eoulz 48. 49. 50, Bt 52, 53 54 7 According to Food Safety Standards Authority of India, which of the following states supply purest milk? (A) Puducherry and Goa (B) Tamilnadu (C) Kamataka and Kerala (D) Andhra Pradesh What is the current level of COs in the atmosphere? (A) 392 ppm (B) 300 ppm (C) 240 ppm (D) 200 ppm Recently, Intemational AIDS Vaccine Initiative (AVI) identified the following finding in AIDS patients: (A) ART (B) Vaccine (C)_ Target binding (D) Neutralizing antibody (vNAbs) Many of the biosynthesized nanoparticles exhibit a common property such as (A) antimicrobial activity (B) X-RD (©) plasmon resonance (D) quantum dot Frederick Sanger who won the Nobel Prize twice discovered (A) 2.3-didoexynucleotide method for DNA sequencing, (B) Labeling of 3’ end of DNA with P” (C) Automatic sequencing (D) Capillary gel sequencing “The whole complex of binding an antibody to 2 bacterial toxin covalently is, called (A)_ immuno complex (8) immuno adhesion (C)_ magic bullet () ricin The heavy metal binding protein, metallothionein, is rich in (A) arginine (B)_ histidine (C)_ methionine (D) cysteine6or12 55 56 97. 58, 59. 60. 61. 62, The first genome to be sequenced was from (A) Haemophilus influenza (B) E.coli (©) Saccharomycetes cerevisae (D) Plasmodium falciparum Which one of the following is a nitrogen-fixing bacterium? (A) Rhizobium (B) Frankia (©) Acetobacter (D) Thiobacillus “The first case of a life patenting was done by (A) Anand Chakrabarty (8) Milstein and Kohler (©) Rolfe () Cocking HIV binds to the following molecule: (a) CD4 (B) CDs (©) MHCI () MHCIL ‘The study of genetic manipulation of a cell leading to overproduction of a particular metabolite is called (A) enzyme engineering (B) metabolic engineering (C)_ metabolic derangement (D) end product inhibition ‘Which one of the following is a radiative gas? (A) Hp (B) O2 (©) CO: ©) ™% Skin colour in man is determined by (A) single gene (B) poly genes (C) alleles (D) mutant genes The human genome consists of the following number of genes: (A) 100,000 (B) 50,000 (©) 30,000 @) 15,000‘Na (eo1i2 J 63 64, 65. 66. 61, 68. 69. 70. ‘The first recombinant protein produced was (A) Thrombin (B) Humilin (C). Factor VII (D) Cyclodextrin Gene therapy is mostly employed against (A) genetic diseases {B) fungal diseases (©) bacterial diseases (D)_ viral diseases Which of the following is a bifunctional enzyme? (A) RuBPCase (B) PEPCase (©) PEPKinase (D) Malic enzyme the GM crop that faces biggest challenge in India for ‘commercialisation is (A) Br-cotton (B) Brmaize (©) Bebrinjal (D)_Be-potato One of the plants that exhibits antimitotic activity a8 2 prelude to treatment of cancer is, (A) onion (B) cluster bean (C)_ soybean (D) cabbage ‘Fo increase drought tolerance in plants, the following gene employed: (A) Mt-D (B) Pro (©) Bet (Dp) HSP “The most popular gene to induce herbicide tolerance 1% plants is (a) LUC (B) GFP (C)_ aroa (Dp) CAT HBSAgis a very popular edible vaccine produced in (A) potato (B) cauliflower (©) sugareane (D)_ beet root‘ENO 60112 1 72, B 74, 75, 16. 77, 10 Enzyme can be engineered using the following technique (A) Site-directed mutagenesis (B) Physical mutagen (©) Chemical mutagen (D) Induction of disulfide bridges In tissue culture the following auxin is primarily required: (A) Myo-inositol (B) IPA © 24D (D) IBA Our coastal regions can be guarded against tsunami more economically by employing (A) blockades (B) concrete walls (C)_ cement blocks (D)_ mangrove afforestation ‘Nucleic acids absorb maximally at (A) 240m (B) 250.nm (©) 260mm (D) 280mm Most higher eukaryotes contain (A) exons (B) introns (©) exons and introns (D) overlapping genes When the final product of a metabolic pathway inhibits the action of the existing enzymes, the process is called (A) repression (B) feedback inhibition (C) catabolic repression (D) uncompetitive inhibition ‘The emerging defense strategy employed in plants against biotic and biotic stresses is (A) abscisic acid (ABA) (B) salicylic acid (SA) (C)_jasmonic acid (JA) (D) ‘ABA-SA-JA inter metabolic pathwayun (eori2 2B. 79. 80. 81 82. 83 84 u In recent times, Multiphoton Fluoroscence Microscope is employed in the detection of (A) cancer (B) jaundice (©) malaria (D) AIDS ‘The most important potentially industrial application of halophilic bacteria is (A) stress tolerant proteases (B) osmoregulatory compounds (C) salinity tolerance (D) stress protective agents Jatropha is considered as a wonder plant because of its (A) drought tolerance (B)_unpalatability to animals (C)_ high oil content in seeds (D) Allof the above Which of the following is a non-leguminous plant to contain leghaemoglobin in Np fixing root nodules? (A) Arachis hypogaea (B) Paraponia andersonii (©) Glycine max (D) Cyamopsis tetragonaloba ‘Transgenic plants accumulating glycine betaine were found to exhibit more tolerance to (A) salinity (B) heavy metals (©) cold (D) high temperature ‘An anti-oxidant vitamin is (A) ascorbic acid (B) biotin (C)_ pantothenic acid (D) deoxy-caleiferol “The light-dependent uptake of O; and evolution of COs in photosynthetic organism is called (A) light reaction (B) dark reaction {C)_ photorespiration (D) oxygenase reactionON EAE O12 86. 87. 88, 89. 1 2 To avoid transgene escape to the environment, genes ate cloned into chloroplast for expression and the process is called (A) vectorless mode of transformation (B) green technology (C)_ metbolic engineering (D) transplastomic technology ‘The antioxidants of green tea specifically prevent (A) cancer (B) jaundice (C)_ hypertension (D) AIDS To distinguish intraspecific genetic variation, the identification tool employed is: (A) RFLP. (B) RAPD (©) DNA bar coding (D) PCR Which of the following is a new threat to the environment? (A) Heavy metals (B) Radioactive wastes (C)_ Radiative gases (D) Wastes Eco RI is isolated from (A) E.coli (B) A. niger (© B.thuringensis (©) fire fly The total genetic material collected and sequenced from an environment is called (A) structural genomics (B)_ metagenomics (© functional genomics (D) genetic diversity The vast majority of life on Earth is (A) plants (B) animals (C) insets (D) microbesQE (az) 92. 93, 95, 96. 97. 13 Im the case of genetic diseases and spinal cord injury, the efficacy of the following therapy is tested: (A) Neural stem cells (B) Embryonic stem cells (C)_ Bone marrow stem cells (D)_ Acombination of all the above “Antisense coat protein gene is used against (A)_ pest resistance (B) viral resistance (C)_ nematode resistance (D)_ fungal resistance Which of the following treatment in the control of malaria is promising and environmentally safe? (A) Pyrethroids (B) Chloroquinone (C)_ Indoor spray of chemicals (D) Artemisinin-based combination therapy ‘The genome of Trichoderma sp. potentially codes for an industrially relevant product (A). Gliotoxin (B) Atmospheric N? fixation (C)_ Aegerolysins (D) Chitinase and glucanase One of the following is a novel ormamental plant commercially grown 1% nurseries: (A) Crotons (B)_ Rose (©) Fems (D) Cycas Helicoverpa armigera is an important pest of (A). brinjal (B) cotton (©) potato (D) papaya98, 99, 100. 101, 102. 104. 105. 14 Given the DNA sequence, ATTAAGGC, which one of the following is the antisense RNA? (A) AUUAAGGC (B) UAAUUCCG (©) CGGAATTA (D) TAATTCC ‘The transgenic plant ‘flavr-savr’ is characterized by (A) delayed ripening (B)_ longer shelf life (C) aroma (D)_Allof the above Photo gene has been identified in (A) nucleus (B)_ mitochondrion (©) cytoplasm (D) chloroplast Which vector is commonly used to express a foreign gene in a dicot plant? (A) Baculovirus vector (B)_ Retroviral vector (C)_ Transposon (D) Tieplasmid ‘Transfer of T-DNA from Ti-plasmid into a plant cell is mediated by (A) MOB genes (B) VIR genes (C) CAT genes (D)_OCT-NOP genes Plant developed from pollen is called (A) monoecious plant (B) di-haploid plant (C)_ sterile plant (D) androgenic plant The production of pharmaceuticals through in vitro culture is called (A)_ metabolic engineering (B) recombinant protein (C)_ biopharming (D) pharmaceutical engineering Restriction endonuclease is employed in cutting (A) single-stranded DNA. (B) double-stranded DNA (C) double-stranded RNA (D) anti-sense RNA‘a (e012) 106. 107. 108. 109. 110. ul 112. 15 Reverse transcriptase is (A) RNA dependent RNA polymerase (B) DNA dependent RNA polymerase (C)_ DNA dependent DNA polymerase (D)_ RNA dependent DNA polymerase ‘The common feature among the nucleus, chloroplast and mitochondrion is (A) lamellae (B) DNA (©) cristae (D) ETC [A technique of using very small metal particles coated with desired gene in gene transfer is called (A)_ electroporation (B)_ microinjection (C)_ biolisties (D) conjugation Which of the following is a stable isotope? (a) of @) ce © re © Which of the following bacteria is involved in the biosorption and detoxification of heavy metals? (A) Pseudomonas aeruginosa (B) Pseudomonas putida (©) Rhizobium japonicum (D) Bradyrhizobium sp. Jn the analysis of heavy metals, which of the following instrument is essentially used? (A) FTIR (B) SDS-PAGE (C)_ Sieve chromatography (D) AFM Rheumatoid arthritis is (A) an auto immune disorder (B) an immunosuppressive reaction (C)_ ahypersensitive reaction (D) aheavy metal-induced disorder60112 13, 114. 11s. 116. 117. 18. 119. 120. ‘A A 16 Nanoparticles can be confirmed by the following method: (A) X-RD (B)_UV-VIS spectrophotometer (©) SEM (D) Allof the above Which bacterium is known as the ‘Work Horse’ in molecular biology? (A) Pseudomonas (B) Acetobacter (©) E.coli (D) Azotobacter Eukaryotic polypeptides are synthesized on the following ribosome complex: (A) 808 (B) 70S (C) 608 (D) 408 Catalytic antibodies are called (A) abzymes (B) ribozymes (C)_ lysozyme (D)_novozymes Harvesting the solar energy is carried out by one of the plastids: (A) Amyloplast (B)_Leucoplast (C) Chromoplast (D) Chloroplast Hairy roots are induced in vitro by the following bacterium: (A) Agrobacterium tumefaciens (B) Agrobacterium rhizogens (©) Rhizobium japonicum (D) Pseudomonas sp. ‘The following one is a non-communicable disease: (A) Diabetes (B) Jaundice (©) Dengue fever (D) Chikungunya ‘According to the Human Genome Project study, what is the percentage of coding genes? (A) 3% (B) 10% © 15% (D) 20%(ACL (wi) 121, 122. 123, 124, 125. 126. 127. 128. 7 ‘The following is the second messenger in plants: (A) Calmodulin (B) Plasmodemma (©) Nodulin (D) Golgi body ‘Somaclones are due to variation in (A) ploidy level (B)_ protein profile (C) plastid number (D)_2.4-D concentration Conversion of CO> to methanol using solar energy is called (A). dark rea (B) photosynthesis (©) light reaction (D) artificial photosynthesis Identification of mud crabs belonging to the genus Scylla is carried out by the following tool: (A) PCR (B) RAPD (©) RFLP (D) Micro array Cryl expression in Bt cotton is affected by the following abiotic stress: (A) water stress (B) salinity stress (©) cold stress (D) heat shock stress The epiphytic lichens act as bio-indicators of (A) pollution (B) atmospheric moisture (©) ambient temperature (D) global warming The normal BP of an individual should be (A) 120/80 (B) 140/90 (C) 110/90 (D) 100/80 ‘The nanoparticles are potent (A) bacteriocide (B) fungicide (©) nematicide (D) _diopesticide60112 129, 130. 131 132. 133. 134 135. 136. 18 The chief proponent of endosymbiosis theory is (A) Rosalind Franklin (B) Darwin (©) Milton (D) Margulies Sexual incompatibility can be overcome by (A)_ In vitro fertilization (B) hybridization (C) cloning (D) cell culture Aromatic amino acids are biosynthesized by a special pathway called (A)_terpenoid pathway (B) shikimic acid pathway (©) elycolate pathway (D) glyoxalate pathway In addition to mitochondria, there is one more power house in plant cells called (A) nucleus (B)_glyoxysome (C) chloroplast (D) peroxisome Against dental caries, the following edible plantibody has been produced: (A) Guy's 13 antibody ®) IgE (© Iga (D) humanized antibody Vinblastin is produced by (A) Rosa indica (B) Vinca rosea (©) Vernonia cinera (D) Victoria regia Spacers are present in (A)__polycistronic mRNA (B) mRNA (©) tRNA (D) RNAi Phylogenetic tree can be constructed by using the following software: (A) clustal W (B) MSa (C)_ gene finder (D) ORF finderA (aon) 19 137, Which one of the following gases plays key role in animal cell culture? (A) COz (B) © HW (D) Os 138, Inthe case of nuclear power plant disaster, the radioactive rays emitted can be detected employing the following instrument: (A) GM Counter (portable) (B) Blotting analysis (C)_ Autoradiography (D) FTIR 139, The most common substrate used in distilleries for the production of ethanol is (A) molasses (B) com meal (©) soya meal (D) _ potato starch 140, The RNAi technology has been used in the development of plants resistant to (A) insects (B) nematode (C) fungi (D) mycoplasma 141, The most important omamental plant decorating many international airports is (A) cycas (B) fem (C)_ orchids (D)_ bonsai 142. The advantage of immobilized enzyme is (A) recycling of the enzyme (B) to avoid enzyme inhibition (C)_ to get pure end product {D) to reduce the Km value 143, Which of the following is a sugar-free plant for diabetic patient? (A). Stevia (8) Gymnema (©) Phyllanthus (D) Curcuma 144, Agar agar is obtained from (A) Agaricus sp. (B) _polyporus (©) Fusarium sp (D) seaweeds601 las. 146. 147. 148. 149. 150. 20 For large scale production of single cell protein, the following mode of culture is resorted to: (A). Petri plate (B) simple culture flask (C) continuous culture (D) bioreactor For disposal of municipal organic wastes, the following tool is used: (A)_ Incineration (B) Vermicompost (C)_ Fermentor (D) Exit remediation Pyrimidine dimer is induced by (A) visible light (B) gamma rays (©) UV rays (D)_ infra-red rays ‘The following enzyme provides protection against UV light in plants: (A)_Isocitrate lyase (B)_Photolyase (C)_ Glycolate oxidase (D) Peroxidase ‘The important function of folic acid metabolism is (A) chlorophyll biosynthesis (B) _Haeme biosynthesis (C) DNA synthesis and repair (D)_ synthesis of cytochrome In the preparation of fast food in the modern world, the following irradiation is ‘made use for safety purposes: (A) UV rays (B) Infra red rays (C). Microwaves (D) Gamma rays