Bng phng php ho hc, phn t enzym c ni li vi nhau to

thnh cc si ngang dc bng phn ng gia cc nhm amin ca enzym vi tc

nhn polymer ho ca mt ho cht nh glutaraldehyd. C nhiu phn ng
ho hc c th s dng bt ng enzym bng cch ny cc nhm chc nng
khng chnh yu ca chng c gn vi cc cht mang v c nh gm, thu
tinh, st, titan ... hoc vi cc polymer thin nhin nh sepharose v cellulose,
vi cc polymer tng hp nh nilon, polyacriamid, cc polymer vinyl khc v
chnh cc polymer ny gi c cc nhm ho chc hot ng tr li. Trong
nhiu th thut thc hin gi enzym vi cht mang bng phng php ny
nhn thy cc nhm hot ng ho hc b phn tn ln xn. V th cc nghin
cu gn y ch n k thut bt ng enzym sao cho cc enzym khi gn
vi cht mang khng b mt hoc gim i hot tnh ca mnh. Gii hn ca k
thut bt ng enzym c trnh by bng 4.5.
Bng 4.5. Nhng hn ch ca k thut bt ng enzym
Phng php

u im

Nhc im

S
gn
kt
ng lng

Khng b nh hng ca
pH, ion ca mi trng
hoc nng c cht

Li hot ng c th bin i gi thnh

t

Polymer
ho
ng lng

Enzym hot ng mnh,

chm mt hot tnh

Hot tnh b mt trong khi khng hiu qu

i vi cc c cht c phn t lng ln;
khng c kh nng ti sinh

Hp ph

n gin, enzym khng b

bin i, c th thu hi
cht mang, gi thnh thp

B thay i trong mi trng ion ho mnh,

enzym b y ra dng cho protease

Enzym khng b bin i

v ho hc

C cht khuch tn vo trong sn phm i

ra ngoi, chun b kh khn, enzym b mt
hot tnh, khng hiu qu i vi c cht
c phn t ln

By
hc

bng

ho

K thut by enzym vo trong cc gel l mt thnh cng khi tin hnh

polymer ho hoc to ht bng cc phn ng ng t. Polyacriamid, collagen,
silicagel ... l nhng cht t ra thch hp cho k thut ny. Tuy nhin qu
trnh to gel kh khn v hiu qu hot tnh ca enzym thp.
Trn thc t thng c khuynh hng bt ng t bo hn l bt ng
enzym v b qua c cng on chit xut v tinh ch enzym mt thi gian,
tn km.
Bt ng t bo ang l vn c tim nng to ln, bi v n cho php
thc hin khng ch i vi vic to ra cc sn phm chuyn ho s cp, m
cn ng dng bin i to ra cc sn phm chuyn ho th cp rt c gi
tr, nh cc khng sinh bn tng hp, cc steroid, iu khin lin tc cc qu
trnh ho hc thng qua cc in cc enzym, x l nc thi, c nh nit...
(bng 4.6).
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