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  • The Effect of Sperm-Oocyte Incubation Time On in Vitro Embryo

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    The Effect of Sperm-oocyte Incubation Time on in Vitro Embryo

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    7 vues9 pages

    The Effect of Sperm-Oocyte Incubation Time On in Vitro Embryo

    Transféré par

    repositoryIPB

    Droits d'auteur :

    © All Rights Reserved
    Téléchargez comme PDF ou lisez en ligne sur Scribd
    Signaler comme contenu inapproprié
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    ‘ELSEVIER ‘Asim Reproduction Science 48 (1997) 187-195 — The effect of sperm—oocyte incubation time on in vitro embryo development using sperm from a tetraparental chimeric bull C. Sumantri *, A. Boediono, M. Ooe, M. Murakami, S. Saha, T. Suzuki United Graduate School of Veteriniry Sciences, Yamaguchi University, Yamaguchi 758, Japan ‘Accepted 27 May 1997 Abstract ‘The present study was designed as 5X 4 factorial to investigate the effects of sng sperm from 5 balls, and varied sperm-oocyte incubation times (5, 10, 15 and 20 h) on the ferilization, cleavage rates ad blastocyst formation onan in vitro bovine embryo production system. The bulls included a tevaparental Chimera, its sires apanese Black and Limousin, its moteralgrand-sires Gapanese Brown and Holstein). The propaction of polyspermy, 2-pronucle formation, friliza- tion, cleavage and development 10 blastocyst were affected (p<0.01) by the duraion of sperm-oocye incubation, as well as by the ineracon between bulls and tir comesponding sperm-oocyte incubation time, Blastacyst cate observed after $ hin oocytes inseminated with Chimera, Japenese Black and Limousin were higher (p <0.05) shan those observed at 20 h incubation, The proportion of blastocyst from oocytes inseminated with Japanese Black observed at 10 h of incubation did not differ from that of Chimera, but bod were higher (p <05) than those observed for he Limousin, Japanese Brown and Holstein ies. The present study showed that there was an effect by the duration of sperm-oocyte incubation on in vitro embryo

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