Module1

1.TITLE:
UsingIndicatorstoExamineProteins,SugarPolymersandSugarMonomers

2.ABSTRACT:

Indicatorsareusedtoshowthepresenceorabsenceofacertainchemical.Inourlabwewillbeexperimentingwithindicatorsto
knowifthereisachemicalinourvialsandnotinourcontrol.Wethink,
If
weuseindicatorstoshowthepresenceofbiological
molecules,
then
therewillbecolorchangeinthevialswithegg,starch,syrupandnotinthecontrols
because
indicatorschange
colorwhentheyfindacertainchemical.MypartnerandIwerewondering,Howcanweuseindicatorstodemonstratethe
presenceofProtein,SugarPolymersandSugarMonomers?Ourproceduresare:puttogetherallthematerialspriortothe
experiment,weargoggles,labcoat,gloves,tiehairupifneeded,getallourpolymers,monomers,tubes,tools,etc,onebyone
dropinyourindicatorontheindependentvariable,writedownourresultsaftereveryindicatorhasbeendroppedandfinally
writedownconclusionifourhypothesisiscorrect.Ourresultisthatour3experimentalvialschangedcolorandthecontrols
stayedthesame.Ourhypothesiswassupported!

3.INTRODUCTION:

Indicators
areusedinlabstogatherinformation.Indicatorsindicate(orshow)thepresenceorabsenceofacertainchemical.
Indicatorsdontactuallychangechemicalstheyjustchangecoloriftheyfindwhattheyarelookingfor.
ChangeColor?
Yes=Foundwhatindicatesfor
No=Didnotfindwhatitindicatesfor
Inourlabwewilluse:

BenedictSolution
:Whichtestfor
sugarmonomers
(singlesugars)(HEATREQUIRED)

Iodine
:Testfor
sugarpolymer
(chainofsugars)(HEATREQUIRED)

Biurett'sSolution
:Testfor
proteins
.(NOHEAT)
Waterwillbeusedasacontrol.
Indicatorsareweakacids.Theweakacidindicatorcausethesolutiontochangecolor(GeneralChemistryOnline).Beforethe
lab,Iobservethattheeggyolkfloatsinthewaterandtheyellowpartisatbottomofthecontainer.MypartnerandIwere
wondering,HowcanweuseindicatorstodemonstratethepresenceofProtein,SugarPolymersandSugarMonomers?

4.HYPOTHESIS:

If
weuseindicatorstoshowthepresenceofbiologicalmolecules,
then
therewillbecolorchangeinthevialswithegg,starch,
syrupandnotinthecontrols
because
indicatorschangecolorwhentheyfindacertainchemical.

5.MATERIALS:

6glasstesttubes
1Erlenmeyerflaskwithwater
3dropperbottleswithBenedict's,IodineandBiurets.
2TestTubeClamps
HeatSource
TestTuberacks
1mlplasticpipets
Egg,syrupandcornstarch

6.PROCEDURE:

1) Puttogetherallthematerialspriortotheexperiment
2) weargoggles,labcoat,glovesetc
3) Tieyourhairupifneeded
4) Getallyourpolymers,monomers,tubes,tools,etcready
5) Onebuyonedropinyourindicatorontheindependentvariable
6) Writedownyourresultsaftereveryindicatorhasbeendropped
7) Writeyourconclusionandcheckifyourhypothesiswascorrect.

7.RESULTS(datatable,graphandPatternStatement2sentences):

NameofIndicator

ColorbeforeHeat

Colorafterheat

Changed?
(Yes/No)

1aBenedictsinSugar

lightblue/lightbrown

orange/orangered

yes

1bBenedictsinH2O

lightblue

lightblue

no

2aIodineinStarch

orangishbrown/
white

darkpurple/black

yes

2bIodineinH2O

red/orange

abitdarkerbutnot
obvious

no

3aBiuretsinprotein

yellow/littlebitof
blue

clear/purple

yes

3bBiuretsinH2O

blue

blue

no

Patternstatement&Evidence:
Our3experimentalvialschangedcolorandthecontrolsstayedthesame.Accordingtoourdata
table,thebenedictinsugar,iodineinstarchandbiuretsinproteinchangecolor,butThebenedictsinH2O,iodinenH2Oand
biuretsinH2Odidnotchangecolor.

8.CONCLUSION(2paragraphs):

Wehypothesizedthatifweuseindicatorstoshowthepresenceofbiologicalmolecule,thentherewillbecolorchangein
thevialswithegg,starch,syrupandnotinthecontrolsbecauseindicatorschangecolorwhentheyfindacertainchemical.Our
hypothesiswassupported!Theegg,starchandsyrupwiththeindicatorschangecolor,buttheegg,starchandsyrupinH2Odid
notchangecolor.Someoferrorsinoutlabswereaddingthemorethan4dropsofprotein,sowehadtostartover.Wealso
accidentallydroppedsomechemicalonthetable,andwipeditwithclothinsteadoftissuepaper.Wealsoheatedoneofour
experimentsfortoolongbecauseIdidnothearmypartnersaystopwhenthetimerstopped.Eventhoughwemadealotoferrors
inourexperiment,Ithinkthatourexperimentisconclusive.Iamveryconfidentinourresultsbecausewefollowedour
proceduresproperlyandwecollectedenoughdata.

Iinferthatthepatternexistbecauseindicatorschangecolorwhentheyfindacertainchemical,butthecontroldidnot
changebecauseweonlyaddedH2Ototheothervialswithegg,starchandsyrup.H2Odoesnotindicatechemicalbecauseits
justordinarywater.Ourfindingsarescientificallybecauseachangeincolorisalsoanothercharacteristicofachemicalreaction.
MypartnerandIwerealsowondering,sincewealreadyknowwhathappenswhenweheatupBenedictinsugar,iodineinstarch
andbiuretsinprotein,whatwillhappenwhenwecoolthemupinstead?Wewerewondering,whatelsecanwedotoexamineif
Proteins,SugarPolymersandSugarMonomershaveacertainchemicalinthem

9.CITATIONS:
GeneralChemistryOnlineThursday25September,2014