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Beetroot Juice on Fatigue/Performance in Exercise

KIN 3250
BY: Jordan, Sarah, Carlee & Salima

Background
Nitrates: therapeutic
applications (Butler et al.,
2008)

Nitric oxide (NO)Multiple methods of


production (Lundberg et al.,
2008)

L-Arginine-NOSNO pathway
Nitrate-Nitrite-NO
pathway
Jones et al., 2014

Background
Dietary nitrate
dependent on
commensal
bacteria (Duncan et
al., 1995; Govoni et al., 2008).

Rapidly
available
following
ingestion

(Webb et

al., 2008)

Webb et al., 2008

Hoon,
2013

TimeArguably
to Exhaustion
vs Time
time trials
Trial
Performance
are
more
appropriate markers
of athletic
performance
Thus time trials are
used to predict
performance

mak et al., 2012 & Lansley et al., 2011

Dietary&
Nitrate
in
Acute
Chronic

500 ml of beetroot
juice has proven to
increase nitrite
concentrations
Examples of studies
NO3
supplementation
have been three
times daily for two
days, three days, six
days, two hours and
2.5 hours prior to
exercise

Bond et al., 2012

Hoon et al., 2013

Beetroot Juice

Performance

There is some evidence to support the idea


that beetroot juice can increase performance
in athletes
In the following areas:
Cycling
Rowing
Kayaking
Sprinting

A randomized double blinded crossover design


was used
Subjects: nine competitive male cycling athletes
Dosage: subjects consumed 0.5L of beetroot juice
(6.2 mmol nitrate) or placebo 2.75 hours before
each TT
Equipment: A computrainer cycle ergometry
system
Exercise: 4 km & 16.1 km TT, 48-72 hour washout
period between trails
Lansley et al., 2011

Results
16km

Lansley et al., 201

4km

Subjects: 20 Trained Male Cyclists


Dosage: 140 ml of beet root juice (8.7
mmol NO3) or placebo 2.5 h before a 1
hour cycling time trial
Double blind repeated measures
crossover design

Cermak et al., 2012

Results

Cermak et al., 2012

Results

Cermak et al., 2012

No Change with
Beet Root
Supplementation

No difference in mean power output in intermittent


sprints to exhaustion with 16 moderately trained
recreational athletes (Martin et al ,2014)

Increase in Plasma Nirate but no difference in VO2 or


in 1500m Time Trial Performance in 8 trained male
runners (Boorsma et al,2014)

No statistically significant improvement in 50 mile


Time Trial performance in 8 well trained male cyclists
(Wilkerson et al, 2014 )

Potential
mechanisms:
Mitochondrial
targets

Larsen et al., 2011

Potential
mechanisms:
Contractile
efficiency

Bailey et al., 2010

Potential mechanisms:
Type 2 muscle fibers

Ferguson et al., 2015

Ferguson et al., 2013

Potential mechanisms: Type


1 and/or 2 muscle fibers?

Bailey et al., 2014

Bentley et al., 2014

Responders and Non


Responders
Potential factors:
Changes in plasma
nitrate (Wilkerson et al., 2012)
Basal NO2
concentrations (Poveda et al.,
1997)

Vascular adaptations
and hypoxia resistance
(Jensen et al., 2004)

Muscle fiber type

(Tesch et al.,

1985; Ferguson et al., 2015)

NOS pathway
adaptations (McConnell et al.,
2007)

Chewing gum/Mouth
wash use (Govoni et al., 2008)

Wilkerson et al., 2012

Should we
recommend
Beetroot Juice?

What are your thoughts?

ight we recommend beetroot to some people and not others

In Conclusion
STUDIES

Bond,2012
Boorsma,201
4
Cermak,2012
Lansley,
2011

IMPROVEME
T IN TIME
TRIAL
PERFORMA
NCE

X
X

Martin, 2014

Peeling, 2014

Thompson,20

Responders vs.
NonResponders

Want more resources


to learn about
Beetroot Juice?

Check out this link:


http://www.todaysdietitian.com/newarchives/0206
12p48.shtml
Read and answer questions: 1-5 & 10

Thank you!

- Carlee, Jordan, Salima &


Sarah

References
Bailey, S. J., Fulford, J., Vanhatalo, A., Winyard, P. G., Blackwell, J. R., DiMenna,
F. J., Wilkerson, D. P., Benjamin, N., & Jones, A. M.(2010) Dietary nitrate
supplementation enhances muscle contractile efficiency during knee-extensor
exercise in humans. Journal of applied physiology. 109(1)135-48
Bailey, S. K., Varnham, R. L., DiMenna, F. J., Breese, B. C., Wylie, L. J., & Jones,
A. M. (2015) Inorganic nitrate supplementation improves muscle oxygenation,
O uptake kinetics, and exercise tolerance at high but not low pedal rates.
Journal of applied physiology. 118(11) 1396-405
Bentley, R., Gray, S. R., Schwarzbauer, C., Dawson, D., Frenneaux, M., & He, J.
(2014) Dietary nitrate reduces skeletal muscle oxygenation response to
physical exercise: a quantitative muscle functional MRI study. Physiological
reports. 2(7) e12089.
Bond, H., Morton, L., & Braakhuis, A. J. (2012) Dietary nitrate supplementation
improves rowing performance in well-trained rowers. International Journal of
Sports Nutrition and Exercise Metabolism. 22(4) 251-56.
Boorsma, R. K., Whitfield, J., & Spriet, L. L. (2014) Beetroot
juicesupplementationdoes not improveperformanceof elite 1500-m runners.
Medicine and science in sports and exercise. 46(12) 2326-34
Butler, A. R., & Feelisch, M. (2008) Therapeutic uses of inorganic nitrite and
nitrate: from the past to the future. Circulation. 117(16) 2151-9

References
Cermak, N. M., Res, P., Stinkens, R., Lundberg, J. O., Gibala, M. J., & Van Loon, L.
J. (2012). No improvement in endurance performance after a single dose of
beetroot juice. International journal of sport nutrition and exercise metabolism.
22(6) 470-8
Duncan, C., Dougall, H., Johnston, P., Green, S., Brogan, R., Leifert, C., Smith, L.,
Golden, M., & Benjamin, N.(1995) Chemical generation of nitric oxide in the
mouth from the enterosalivary circulation of dietary nitrate. Nature Medicine.
1(6) 546551.
Ferguson, S. K., Hirai, D.M., Copp, S. W., Holdsworth, C. T., Allen, J. D., Jones, A.
M., Musch, T. I., & Poole, D.C. (2013) Impact of dietary nitrate supplementation
via beetroot juice on exercising muscle vascular control in rats. The journal of
physiology. 591(2) 547-57
Ferguson, S.K., Holdsworth, C.T., Wright, J.L., Fees, A.J., Allen, J.D., Jones, A.M.,
Musch, T.I., & Poole, D.C. (2015) Microvascular oxygen pressures in muscles
comprised of different fiber types: Impact ofdietarynitrate supplementation.
Nitric oxide. 48. 38-43
Govoni, M., Jansson, E. A., Weitzberg, E., & Lundberg, J. O. (2008) The increase
in plasma nitrite after a dietary nitrate load is markedly attenuated by an
antibacterial mouthwash. Nitric oxide. 19(4) 333-7
Hoon, W. M., Johnson, A. N., Chapman, G. P., & Burke, M. L. (2013). The effect of
nitrate supplementation on exercise performance in healthy individuals: a
systematic review and meta-analysis. International Journal of Sports Nutrition
and Exercise Metabolism. 23(5), 522-532.

References
Jensen, L., Bangsbo, J., &Hellsten, Y. (2004) Effect of high intensity
training on capillarization and presence of angiogenic factors in human
skeletal muscle.Journal of Physiology.557 (2) 57182.
Jones, A., M. (2014) Dietary Nitrate Supplementation and Exercise
Performance. Sports med. 44(Suppl 1) 35-45.
Lansley, K. E., Winyard, P. G., Bailey, S. J., Vanhatalo, A., Wilkerson, D.
P., Blackwell, J. R., & Jones, A. M. (2011). Acute dietary nitrate
supplementation improves cycling time trial performance. Medicine &
Science in Sport & Exercise. 43(6), 112531.
Larsen, F. J., Schiffer, T. A., Borniquel, S., Sahlin, K., Ekblom, B.,
Lundberg, J. L., Weitzberg, E. (2011) Dietary inorganic nitrate improves
mitochondrial efficiency in humans. Cell metabolism. 13(2) 149-59
Lundberg J., O., Weitzberg, E., & Gladwin M., T. (2008) The nitrate
nitritenitric oxide pathway in physiology and therapeutics. Nature
reviews. 7(2) 156-67
Martin, K., Smee, D., Thompson, K., Rattray, B. (2014) No Improvement
of Repeated-Sprint performance with Dietary Nitrate. International
Journal of Sports Physiology and Performance. 9(5) 845-50

References
McConell, G. K., Bradley, S. J., Stephens, T. J., Canny, B. J., Kingwell, B.
A., & Lee-Young, R. S. (2007) Skeletal muscle nNOS protein content is
increased by exercise training in humans.American journal of
physiology.293(2) R821R828.
Poveda, J. J., Riestra , A., Salas, E., Cagigas, M. L., Lopez-Somoza, C.,
Amado, J.A., & Berrazueta, J.R. (1997) Contribution of nitric oxide to
exercise-induced changes in healthy volunteers: effects of acute
exercise and long-term physical training.European journal of clinical
investigation.27(11) 96771.
Tesch, P. A., & Karlsson, J. (1985) Muscle fiber types and size in
trained and untrained muscles of elite athletes.Journal of Applied
Physiology.59(6) 171620.
Webb, A. J., Patel, N., Loukogeorgakis, S., et al. (2008) Acute blood
pressure lowering, vasoprotective, and antiplatelet properties of
dietary nitrate via bioconversion to nitrite. Hypertension. 51(3):784
790.
Wilkerson, D. P., Hayward, G. M., Bailey, S. J., Vanhatalo, A., Blackwell,
J. R., & Jones, A. M.(2012) Influence of acute dietary nitrate
supplementation on 50 mile time trial performance in well-trained
cyclists. European journal of applied physiology. 112(12) 4127-34

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