DiffusionandOsmosisLab

11/5/15
LabPartners:Brianna,John,Kiana,Dominique

DiffusionandOsmosisLab

Question:Whatarethesucrosemolaritiesof6unknownsolutions?
Variables:
Independent:dialysistubing
Dependent:solutions
Constant:roomtemp,watertemp
MaterialsandProcedure:(seelabhandout)

OsmosisinDialysisBags
Weighboatmass:5.8g
Contentsof
DialysisBag

InitialMass
(grams)

FinalMass
(grams)

Mass
Difference

Percent
Change

Molarity

Yellow

16.45

17.60

1.15

6.99%

0.8

Orange

18.20

17.60

0.35

1.92%

0.0

Red

15.40

15.80

0.40

2.59%

0.4

Blue

14.50

14.50

0.00

0.00%

0.2

Green

15.70

16.75

1.05

6.68%

0.6

Purple

15.55

18.10

2.55

16.39%

1.0

ClassMeanData
DialysisTubing

ClassMeanPercentChange

Molarity

Red

5.8

0.4

Orange

1.0

0.0

Yellow

10.4

0.8

Green

8.0

0.6

Blue

2.1

0.2

Purple

14.2

1.0

DialysisQuestions
1. Asthemolarityofthesucrosesolutionsinthedialysisbagsincreased,themasschange
increasedaswell.Thisisduetoasconcentrationincreases,thesolutepotential

2.

3.
4.
5.

decreases,thusdecreasingthewaterpotentialinthebag.Thiscausesahypotonic
environmentpullingwaterintothebag,duetomorespaceopeningupforthebags.
The0.4Mbagwouldnotexperienceanymasschangeforthebagandthewaterwould
beisotonicduetohavingequalwaterpotentials.Themassofthebagswithmolarities
lowerthan0.4Mwoulddecreaseasthewatersolutionwouldhavealowerwater
potential,thanthedialysisbagdrawingwateroutofthebag,duetomorespacebeing
openinthesolutionforwatertofill.Thosewithamolarityabove.4Mthereverse
situationwouldbetruewiththebaghavingthelowerwaterpotentialthanthewater
solutiondrawingwaterfromthesolutionintothebag.
Therewasarangeofvolumesthatwereusedineachbagmakingthemasschange
possiblydeceiving,whilethepeernetchangewouldbemoreaccurate.
(1820)/20X100=10%
Hypertonic

Conclusion:Theconcentrationsforthesolutionswereasfollows:yellow0.8,orange0.0,red
0.4,blue0.2,green0.6,andpurple1.0.Theconcentrationwasdeterminedbylookingatthe
percentchangeofthefinalinitialmassandassigningthehighestmolaritytothehighest
percentchangeandsoonfortherestofthesolutions.Astheconcentrationincreasesthesolute
potentialdecreases(morenegative)aswellthuscausingadecreaseinthewaterpotentialthe
higherwaterpotentialsexistedintheareawithlowerornoconcentrationofsolute,withthe
lowerwaterpotentialbeinginthebagsorsolutionswiththehighersoluteconcentration.This
wasreflectedinthegraphforthemoreconcentratedthedialysisbagthegreaterthemass
change.Errorsthatmayhaveoccurredwhileconductingthelabcouldbeincorrectlyfillingthe
dialysistubing,notrinsingtheweighboataftertakingthemassofallofthebags,andnottaking
themassaccurately.

Question:Whatisthemolarconcentrationofthesolutewithinthepotatocores?
Variables:
Independent:potatocores
Dependent:solutions
Constant:roomtemp,solutiontemp
MaterialandProcedure:(seelabhandout)

Weighboat:5.8,5.7
Colorof
Solution

InitialMass
(grams)

FinalMass
(grams)

Mass
Difference

Percent
Change

Molarity

Red

3.60

2.80

0.80

22.22

0.4

Orange

3.40

4.05

0.65

19.11

0.0

Yellow

3.20

1.95

1.25

39.06

0.8

Blue

3.25

3.30

0.05

1.53

0.6

Green

2.70

1.70

1.00

37.03

0.2

Purple

2.70

1.70

1.00

37.03

1.0

ClassMeanData

PotatoCores

ClassMeanPercentChange

Molarity

Red

20.1

0.4

Orange

17.7

0.0

Yellow

40.1

0.8

Green

39.3

0.6

Blue

2.3

0.2

Purple

34.1

1.0


PotatoCoreQuestions
1. Aswaterisleavingthepotato,theconcentrationofthepotatocellsincreasesasthe
solutesconcentrationwouldbecomemorenegativesothewaterpotentialwould
becomelowerwithinthepotatocells.
2. Thecellwouldbehypotonic,anditwillgainwater.Thecellwillhavelessfreemoving
watermoleculesloweringitswaterpotentialbelowthatoftheenvironmentsurroundingit
makingitverylikelyforwatermoleculestomoveintothecell.
3. Thepressurepotentialisshowntobezero
4. Dialysisbag

5. Outof,becausethewaterwillwanttomovefromanareaofhighwaterpotentialtoan
lowerareaofwaterpotential,duetothespaceopenedupforthewatertofill.

8.a.8.975bars
b.1(.36m)(.0831)(300)=8.975bars
9.Solutepotentialisthemeasureofchangeinwaterpotential,ifmoresolutesarepresentina
systemthenthesolutepotentialbecomeslowerandmorenegative
10.a.distilledwater
b.distilledwater
c.Distilledwaterisahypotonicsolution,ifaredbloodcellisplacedwithinthissolutionit
wouldmeanthatthewaterconcentrationishigheroutsidethecell,andlowerinside.Waterwill
movefromahighertolowerconcentrationduetoosmosis,thereforethecellwillbefilledyetit
canonlyhavesomuchintakeand,sothecellislikelytoburstduetotheamountofpressure.
11.Thegrassneartheroaddieswhensaltedbecausethewateringrasswillmovetoa
higherconcentrationofsoluteinordertoreachequilibrium.Andsincethesalthasaveryhigh
soluteconcentration,itdoesn'tanywaterforthegrasstosurvive.