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DNA Structure and Replication

Douglas Wilkin, Ph.D.

Jean Brainard, Ph.D.

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Printed: November 6, 2015

Douglas Wilkin, Ph.D.
Jean Brainard, Ph.D.



Chapter 1. DNA Structure and Replication

DNA Structure and


Summarize Chargaffs rules.

Explain the findings of James Watson and Francis Crick.
Define nucleotide.
Summarize the process of semi-conservative DNA replication.

How do these four structures form DNA?

In an extremely elegant model, thats how. As you will soon see, the model predicts how the DNA sequence can
code for proteins, and how the molecule can be replicated.
DNA Structure and Replication
Chargaffs Rules

Other important discoveries about DNA were made in the mid-1900s by Erwin Chargaff. He studied DNA from
many different species. He was especially interested in the four different nitrogen bases of DNA: adenine (A),
guanine (G), cytosine (C), and thymine (T) (see Figure 1.1). Chargaff found that concentrations of the four bases
differed from one species to another. However, within each species, the concentration of adenine was always about
the same as the concentration of thymine. The same was true of the concentrations of guanine and cytosine. These
observations came to be known as Chargaffs rules. The significance of the rules would not be revealed until the
structure of DNA was discovered.
The Double Helix

After DNA was found to be the genetic material, scientists wanted to learn more about it. James Watson and Francis
Crick are usually given credit for discovering that DNA has a double helix shape, like a spiral staircase (see Figure


Nitrogen Bases in DNA. The DNA of all species has the same four nitrogen bases.

1.2). The discovery was based on the prior work of Rosalind Franklin and other scientists, who had used X rays to
learn more about DNAs structure. Franklin and these other scientists have not always been given credit for their
contributions. You can learn more about Franklins work by watching the video at this link: http://www.youtube.c
om/watch?v=s3whouvZYG8 (7:47).
The double helix shape of DNA, together with Chargaffs rules, led to a better understanding of DNA. DNA, as a
nucleic acid, is made from nucleotide monomers, and the DNA double helix consists of two polynucleotide chains.
Each nucleotide consists of a sugar (deoxyribose), a phosphate group, and a nitrogen-containing base (A, C, G, or
Scientists concluded that bonds (hydrogen bonds) between complementary bases hold together the two polynucleotide chains of DNA. Adenine always bonds with its complementary base, thymine. Cytosine always bonds with
its complementary base, guanine. If you look at the nitrogen bases in Figure 1.1, you will see why. Adenine and
guanine have a two-ring structure. Cytosine and thymine have just one ring. If adenine were to bind with guanine
and cytosine with thymine, the distance between the two DNA chains would be variable. However, when a one-ring
molecule binds with a two-ring molecule, the distance between the two chains is kept constant. This maintains the
uniform shape of the DNA double helix. These base pairs (A-T or G-C) stick into the middle of the double helix,
forming, in essence, the steps of the spiral staircase.

DNA Replication

Knowledge of DNAs structure helped scientists understand how DNA replicates. DNA replication is the process in
which DNA is copied. It occurs during the synthesis (S) phase of the eukaryotic cell cycle. DNA replication begins
when an enzyme, DNA helicase, breaks the bonds between complementary bases in DNA (see Figure 1.3). This


Chapter 1. DNA Structure and Replication

The DNA molecule has a double helix

This is the same basic shape

as a spiral staircase.

Do you see the

resemblance? Which parts of the DNA

molecule are like the steps of the spiral

exposes the bases inside the molecule so they can be read by another enzyme, DNA polymerase, and used to build
two new DNA strands with complementary bases, also by DNA polymerase. The two daughter molecules that result
each contain one strand from the parent molecule and one new strand that is complementary to it. As a result, the
two daughter molecules are both identical to the parent molecule. DNA replication is a semi-conservative process
because half of the parent DNA molecule is conserved in each of the two daughter DNA molecules.
The process of DNA replication is actually much more complex than this simple summary. You can see a detailed
animation of the process at this link: http://www.youtube.com/watch?v=-mtLXpgjHL0 (2:05).


Chargaffs rules state that the amount of A is similar to the amount of T, and the amount of G is similar to the
amount of C.
Watson and Crick discovered that DNA has a double helix shape, consisting of two polynucleotide chains held
together by bonds between complementary bases.
DNA replication is semi-conservative: half of the parent DNA molecule is conserved in each of the two
daughter DNA molecules.

Making Connections

Click image to the left or use the URL below.
URL: http://www.ck12.org/flx/render/embeddedobject/115600


DNA Replication.

DNA replication is

a semi-conservative process.

Half of

the parent DNA molecule is conserved

in each of the two daughter DNA

Explore More

Use these resources to answer the questions that follow.

Explore More I

http://www.hippocampus.org/Biology Non-Majors Biology Search: DNA Structure and Function


Describe the structure of DNA.

The phrase sides of the ladder refers to what structure(s)?
Why is there a specific pairing pattern among the bases?
Why are the two strands of the double helix perfect and specific compliments?
List three functions of DNA that are based on its structure.

Explore More II

http://www.hippocampus.org/Biology Non-Majors Biology Search: Replication



Chapter 1. DNA Structure and Replication

Why must DNA be replicated?

When does replication occur?
Describe the first step of replication.
Why is each strand of DNA able to serve as a template for replication?
Explain the meaning of semi-conservative replication.

Explore More III

DNA Replication at http://johnkyrk.com/DNAreplic.swf .

Build a DNA Molecule at http://learn.genetics.utah.edu/content/begin/dna/builddna/ .


What are Chargaffs rules?

Identify the structure of the DNA molecule.
What are nucleotides? What makes up a nucleotide?
Why is DNA replication said to be semi-conservative?
Create a diagram that shows how DNA replication occurs.
What is complementary base pairing? Explain why complementary base pairing is necessary to maintain the
double helix shape of the DNA molecule.

1. Hana Zavadska. Shows the four nitrogen bases that make up the DNA of all species . CC BY-NC 3.0
2. DNA: Image copyright ermess, 2014; Staircase: Andrew Gould. Compares the shape of a DNA molecule wit
h a spiral staircase . DNA: Used under license from Shutterstock.com; Staircase: CC BY 2.0
3. Madeleine Price Ball. A simple illustration of the DNA replication process . Public Domain