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Date: 11-5-15
Safety: Do not get sucrose solution
Procedure: See Lab Handout
Materials: See Lab Handout
Diffusion pre-lab:
Graph:
Questions:
1. The higher molar concentration in the beakers caused a
larger concentration gradient into the dialysis bags causing its
mass to increase.
2. If all of the bags were placed into a 0.4 m sucrose solution
the 0.0 m and 0.2 m sucrose solutions would decrease in size to
try to fix the concentration gradient, the 0.6 m 0.8 m 1.0 m
sucrose solution would increase in size to fix the concentration
gradient, and the 0.4 m sucrose solution would remain the same
because it is the same amount of solution in each bag.
3. All the initial values were different, so to get a more
accurate representation of how much they increased, a
percentage was used.
4. 20grams to 18g= -2g change, -2/20= -10% change
5. Hypertonic
Conclusion: After the lab, we determined that the molar
concentrations of the solutions go as follows: Red is .4M, Orange is
0M, Yellow is .8M, Green is .6M, Blue is .2M, and Purple is 1.0M. We
determined the concentrations by Water and solute potential of the
solutions. The one with more solute concentration didn't have as
much water potential which meant the mass of the bags changed less
with more. In the 0m concentration, the solution outside of the bag
had a lower water potential than insider which meant that the
concentration gradient made water flow out of the bag, causing a
decrease in mass inside the bag. The rest of the concentrations had a
higher water potential and lower water potential than the bag, which
caused the concentration gradient to flip and make water flow into
the bags because of the low water potential. Our graph reflects how
the lower concentrated solutions increased less, and even decreased,
compared to the higher concentrations. Our hypothesis was correct
according to our findings. The more sugar in the bag, means a larger
solute potential and a smaller water potential, meaning that the mass
increases more due to the concentration gradient. Our results may
have been slightly altered by the time the bags were left out of the
water, which may have led to some evaporation of the water, altering
the water potential, and the concentration gradient. This could be
solved by having more people tying bags so that the time out of
solution is minimal and decreases the chance of the evaporation.
1C: Potato Cores
Variables:
-Independent: Molar Concentration of solution
Graph:
6.
7.
8.
8b.