Study of Quantity of Casein

Present in Various Samples of

Milk

Index
1.Introduction

2.Requirements
3.Procedure
4.Observation
5.Result and discussion
6.Bibliography

Introduction
Milk is a white liquid secreted by the
mammary glands of female cows, buffaloes
etc. It is a food of exceptional inters
probability. Milk is the most nutritious food
found in nature. That is why it is widely
consumed both in its natural forms and as
diary product like butter, cheese etc. Milk
contains vitamins, proteins, carbohydrates and
the major milk protein casein are found only
in milk and nowhere else in nature. The
Composition of casein in milk is not constant.
It depends upon the source of milk.

Theory
The protein, casein, existent, milk as the
calcium salt, calcium casein etc. The salt has a
complex structure and form molecules. These
molecules have negative charges on the outer
surface .

Requirements
250-ml. beaker
Filtrations flask
Measuring cylinder
Glass rod
Spatula
China dish
Dropper
Weight bore
Different samples of milk
10% acetic acid

Procedure
1.Measure 20-ml, of a milk sample

with the help of measuring cylinder

and transfer into a clean 250ml.
beaker.
2.Make the volume of milk to about
35ml, by adding water and warm
the mixture to about 40 c.
3.Add to the warm mixture drowse
and

with

constant

stirring

10%

acetic acid unit the precipitation is

completed.

4.Stir the mixture for another 10

minutes

or

till

the

precipitated

casein forms a large amorphous

mass.

5.Filter the casein precipitate.

6.Wash thoroughly with water, dry

foils between folds of filter paper
and then in air for 1-2 days weight
the dry solid so obtained.
7.Similarly, determine the amount of
casein in other samples of milk.

Observation
Volume of Milk

Amount of % of

Milk

casein

brand

Taken in

casein

found

each case
= 20ml.
Sample
No.
1.
2.

Saras
Mother

0.60
0.65

3.00%
3.25%

3.
4.

diary
Amul
Ananda

0.85
0.75

4.20%
3.70

Results and
discussion
Different samples of milk contain different
percent of casein and the casein contents in
Amul brand are appreciable. At present Amul
is the best brand of milk available in the
market.

Bibliography

Laboratory manual in chemistry

Class XII by B.Bhushan.

Advanced organic practical chemistry for
P.G. Students & by O.P. Aggarwal.