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Starch
Yes
Iodine
Yes
Background Questions:
1. What is the difference between osmosis, diffusion and facilitated
diffusion? Facilitated diffusion is diffusion using carrier or channel
proteins in the cell membrane that assist in the movement of molecules
across a concentration gradient. Osmosis has to do with the movement of
only water.
Procedure:
A. Diffusion through a Membrane:
1. Obtain a small plastic cup. Go to the counter and put three
droppers full of starch and three droppers full of glucose into the cup.
2. Set up a dialysis tubing Cell like the one Ms. V demonstrated.
3. Fill the tubing cell with three droppers full of iodine. Tie the top
of the bag closed.
4. Rinse the cell bag under running water.
5. Make an initial observation in the date table 1.Record your
observations for the color of the cup solution and the color of the bag
under 0 minutes.
6. Check the bag and the cup every 10 minutes for 30 minutes and
record your observations in the data table.
7. After your last observation at 30 minutes, take out the bag and
cut the top off. Pour the contents of the bag into a test tube.
8. Add 15 drops of Benedicts solution to the tube. Place the tube in
a boiling water bath and watch for a color change to orange for a
positive glucose test. IF THE TUBE IS ANY OTHER COLOR THAN
ORANGE, THE RESULT IS NEGATIVE. Record the results in cell
membrane data table 1.
9. Record the results of the glucose test.
10.CLEAN ALL THE GLASSWARE AND THROW ALL YOUR TRASH
AWAY. CLEAN UP YOUR LAB AREA.
B. Cell Simulation
1. Go to the front of the room and make two cells from the dialysis
tubing in the beaker marked Cell Membranes.
2. Tie one end of the cell off with string and fill the inside of cell
number 1 with 3 droppers full of fluid from the beaker marked Cellular
Fluid.
3. Tie off the other end of the cell.
4. Measure the weight of the cell on the balance. Record the weight
of the bag in data table 2.
5. Record the color of the cellular fluid in the cell in the data table.
6. Place the cell into a clean plastic cup.
7. Fill the cup with just enough soda to cover the cell.
8. For cell number two, put three droppers full of soda into the cell
bag and tie it off securely.
9. Weigh cell number two on the balance and record its weight in
data table 2.
10.Place cell two in a plastic cup and this time cover the cell with
just enough cellular fluid solution.
11.Let the bags sit in the cups for 25 minutes.
12.While you are waiting, do the Plasmolysis demonstration below.
13.After 25 minutes, remove the bags from the cup and dry it off
with paper towel.
14.Record the color of the fluid in the bags and the color of the
fluids in the cups.
15.Weigh the cells on the balance and record the weights in data
table 2.
16.Wash out the cup and put it away and throw the cell away in the
trash.
17. Answer your hypothesis based on your observations and data in
your conclusion.
C. Plasmolysis Demonstration
1. Get a small piece of red onion skin. MAKE SURE ITS FROM THE
RED SIDE NOT THE CLEAR SIDE.
2. Place the skin onto a clean slide. Make a wet mount slide out of
the onion and add a cover slip.
3. Using good microscope technique, focus the slide on scanning
power.
4. Switch to low power and sharpen the focus.
5. Switch to high power and observe two or three onion cells.
6. Draw the two or three cells on white paper and be sure to
include the red portion of the cell membrane in your drawing.
7. Remove the cover slip from the slide.
8. Take the slide to the front and add a drop of saltwater to the
onion.
9. Replace the cover slip and let the slide sit for 5 minutes.
10.After 5 minutes, re-focus the slide and find three cells on high
power.
11.Draw two or three cells on high power. Be sure to draw the red
cell membranes and where they are located on the cell. Go to the data
10
Purple
Brown
20
Purple
Brown
30
Brow
Brown
Final Weight
(g)
5.5
Initial Color of
Cellular Fluid
Clear
Final Color of
Cellular Fluid
light Brown
6.3
Brown soda
color
Brown
5.5
5.7
Jonah:
If we experiment to see whether the soda and cellular fluid will diffuse then the
soda will be able to pass through the membrane however the cellular fluid will not
because the soda is acidic and will be able to pass through. When we tested if the
iodine, starch, or glucose will get past the membrane, only the iodine and starch
could pass over. The glucose will not diffuse because it is hard to digest. My
partners predictions were correct for test A however they were not very accurate
for Test B. For test B, the glucose did pass over the membrane however the
iodine and starch did not. It is very crucial to have diffusion in living organisms
because it is how things enter the body and how product are removed.
This was late because I was sick for a week.