CHM260 LABORATORY REPORT

EXPERIMENT 2
UV-VISIBLE DETERMINATION OF AN UNKNOWN
CONCENTRATION OF KMnO4 SOLUTION.

NAME:
STUDENT ID:
GROUP:
DATE OF REPORT SUBMISSION:
LECTURERS NAME:

PROCEDURE
A. Preparation of the KMnO4 Standard Solutions
1. 0.01 KMnO4 was weighed on a weighing paper. Then was transferred into 100mL volumetric
flask.
2. The solid was dissolved with distilled water to the mark. The mixture was shaken to produce
3.
4.
5.
6.

homogenous solution.
The stock was pour into a beaker.
5mL of the stock was pipette and dilute with distilled water in a 100mL volumetric flask.
The mixture was transfer into a beaker.
Step 4 was repeated with 10mL, 15mL, 20mL stock solution and was transfer into a small
beaker.

B. Preparation of the Unknown.

1. The stock was pipette between 5-20mL of the stock KMnO4 solution and diluted with distilled
water in a 100mL volumetric flask.
2. The mixture was transfer into a beaker.
C. Determination of absorption Maximum(max)
1. The absorbance reading was starting with least concentrated and gradually increasing by
using spectrophotometers.
D. Operation of the UV-Visible Spectrophotometer
1.Carry Win UV icon was selected.
2.Scan icon was clicked.
3.Setup, CARY was click. Then, start and stop scan was key in.
1) Y-mode (min=0 and max=1)
2) X-mode =800-200nm
3) Beam mode = Dual beam
4. Cycle mode was selected if more than 1 cycle is required.
5. Scan Control Speed was selected to fast
6. Baseline was click, and then baseline correction function was check.
7. Accessories 1 were click and Use Cell Changer was check to select cells.
8. Maximum peak was selected at the peak option.
9. Auto Store icon, Storage on (Prompt at Start) was set.
10. To save method, file was click and saved the scan method.
11. The blank cuvette was filled with distilled water.
12. The cuvette then was put into the instrument and baseline was click.
13. The blank cuvette was removing and sample cuvette was put.

14. The icon start was click to start the measurement.

E. Determination of the Unknown Concentration
1. The concentration icon was clicked.
2. Set up and CARY was clicked, then maximum wavelength was key in.
3. Replicate = 3 was selected.
4. The standard icon was click, and Calibrate During Run function was checked.
5. The calibration standard unit and the number of standard samples were set.
6. Fit type was selected.
7. Sample icon was clicked and key in the number of samples and unknown.
8. Report icon, the operator name and the comment was key in.
9. Auto Store icon, Storage on (Prompt at Start) was set.
10. File-Save Method as-Ok was click to save method.
11. Blank cuvette was put and ZERO was click.
12. Removed the blank and was replaces with sample cuvettes.
13. Start icon was clicked to start the concentration measurement.

DATASHEET EXPERIMENT 2

UV-VISIBLE DETERMINATION OF AN UNKNOWN CONCENTRATION OF KMnO4

SOLUTION.

NAME:

DATE:

STUDENT ID:

GROUP:

Solution

Concentration in (ppm)

Absorbance

Standard 1
Standard 2
Standard 3
Standard 4
Unknown
Mass of KMnO4=__________g

max=__________

Show the sample calculation for the preparation of standard 3:

Concentration of unknown:_______________