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Yeast Signal

Transduction Lab
Saccharomyces
cerevisiae

Yeast are

Eukaryotes
Single celled organisms
Kingdom Fungi
Commonly used in biological experiments
Also used in commercial applications such
as bread and wine preparation

Fungi vs. Bacteria


FUNGI
Eukaryote
Unicellular or
Multicellular
Cell Wall
Never photosynthetic

BACTERIA
Prokaryote
Always Unicellular
Cell Wall
Can be photosynthetic

Haploid Strains of Yeast


MAT a
Release a factor pheromones
Have receptors for factor

MAT
Release factor pheromones
Have receptors for a factor

Yeast Mating
When the appropriate factors are released, a
MAT and MAT a cell can mate
The binding of the pheromone to the
receptor causes a signal transduction
pathway to be activated.
Cells start to grow towards one another
they shmoo!

Diploid vs. Haploid


What are the advantages of being diploid?
2 copies of every gene
Allows you to compensate for a bad gene

Growth of Yeast
YED media
Complete media
Any yeast should be able to grow on it.

MV media
Minimal media
Lacks some nutrients that yeast might need
If a yeast cell cant make these nutrients
themselves, they cant grow on MV media

Yeast mutants
Some yeast contain mutations that do not
allow them to produce specific compounds
= auxotrophic
When a yeast mutant is auxotrophic for a
specific compound, it will be unable to
grow on MV media.

Yeast mutants (continued)


In order for an auxotrophic yeast to grow
The compound has to be added to the media
OR
The yeast must mate with another yeast strain
that can complement it (so it can make the
compound) = complementation

Lab Experiment
Week 1: Mate haploid yeast and observe
growth on MV media (to show
complementation), observe shmooing
Week 2: Determine the defect in haploid
yeast strains through mating and
complementation on MV media

Your homework:
Study for the Pre Lab Quiz
Set up lab notebook:

Write a SPECIFIC title


Write an Intro (2-5 sentences)
Paste in procedure
Make a flowchart for EACH page of the
procedure