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INTRODUCTION
Reproductive modes and life cycles on organisms:
1. Asexual no sexual reproduction
2. Alternate between short periods of sexual and long periods of
asexual reproduction
3. Diploid eukaryotes sexual reproduction is evident
Sexual reproduction produce new members of species; orderly
transmission of genetic material from parents to offspring
Phenotypic variability due to segregation and independent
assortment during meiosis
Zea mays
Many plant life cycles alternate
between haploid gametophyte
stage and diploid sporophyte stage
- linked by meiosis and fertilization
Maize (corn) monoecious seed
plant; sporophyte phase dominate
in the life cycle
Both male and female structures are
present on the adult plant
1. Stamens (collectively constitute the tassel) produce diploid microspore mother cells
2. Microspore mother cell undergoes meiosis gives rise to 4 haploid microspores
3. Haploid microspores develop into mature male microgametophyte pollen grain (2 haploid sperm
nuclei)
4. Pistil produces diploid megaspore mother cells
5. Megaspore mother cell undergoes meiosis 1 of the 4 haploid megaspores survive
6. Haploid megaspore undergoes mitosis produces 8 haploid nuclei enclosed in embryo sac
7. 2 of the nuclei unite near the center becomes endosperm nuclei
8. 3 of the nuclei at micropyle end (where sperm enters) the oocyte and 2 synergids
9. 3 other nuclei antipodal nuclei; at the opposite end of the embryo sac
10.Pollination occurs when pollen grains make contact with stigma of the pistil
Mutant genes that disrupt normal tassel and pistil formation normal
products of the mutant genes affect the differentiation of male or
female tissues
Mutations of tassel seed produces only female structures
Mutations of silkless and barren stalk produces only malefunctioning reproductive organs
Caenorhabditis elegans
Beneficial in investigating genetic control of development
2 sexual phenotypes: males having testes only, hermaphrodites
During hermaphrodite larval development testes form producing
sperm is stored; oogenesis does not occur until adult stage is reached
Eggs produced are fertilized by stored sperm through self-fertilization
Offspring hermaphrodites (less than 1 percent are males)
Males can mate with hermaphrodites produces half male, half
hermaphrodites
Barr Bodies
Barr and Bertram
Experiments with female cats
Observed darkly staining body in interphase of nerve cells absent in
males (can be found in human female buccal mucosa or fibroblasts)
Stains positively for number of different DNA-binding dyes
Barr body
Sex chromatin body
Inactivated X chromosome provides possible mechanism for
dosage compensation; Arises from one the 2 X chromosomes
X genetic dosage will be same for male and females
Lyon Hypothesis
Mary Lyon and Liane Russell
Inactivation of X chromosomes occur randomly in somatic cells at a
point early in embryonic development (during blastocyst)
When inactivated all descendant cells have same X chromosome
inactivated as initial progenitor cell
Based on observations of female mice heterozygous for X-linked
coat color genes - 2 colors due to expression of alleles in both X
chromosome
Mechanism of Inactivation
Imprinting
DNA, attached histone proteins, or both are chemically modified
silencing most genes part of the chromosome
A memory is created keeping the same homolog inactivated
following chromosome replications and cell divisions
Genetic information is modified and gene expression is repressed
Epigenetics
Mechanism of Inactivation
Region of mammalian X chromosome major control unit (on
proximal end of p arms)
X inactivation center (Xic) genetic expression occurs only on X
chromosome that is inactivated; 1 MB (106 base pairs)
Contain putative regulatory units and 4 genes one of it is the Xinactive specific transcript (XIST); critical gene for X-inactivation
Case 2
Case 3
Observations:
1. Certain temperatures result to both male and female offspring
2. Pivotal temperature (Tp) range is fairly narrow (less than 5oC)
Involvement of steroids (estrogen) and enzymes involved in synthesis
on the effect of temperature in sex
Aromatase converts androgens (testosterone) to estrogen; activity
is correlated with pathway of reactions occurring during gonadal
differentiation