Forensics Application

Presentation
Sarah Cates Gigi Emerson

Background on Situation

Dr. Doodles Donleys laboratory investigated bacterial and viral pathogens.

Dr. Donley had recently been honored with the prestigious Scientist of the Year award and she was throwing a party
to honor herself. The entire University was invited.
At around 2 am, the night security guard was making his rounds, and he noticed a light on in Dr. Donleys lab. The
place was a messthe remains of a rockin party was obvious, but Dr. Donleys bruised and battered body was a
surprise.
The security guard called the Monarch University police at 2:05 am. The police determined that several vials were
missing from the level 4 containment room and Dr. Donley was presumed to have been attacked by some sort of foul
play.
Apparently Doodles was an expert in the field of microbiology. In other words, she studied virii and bacteria. So far it
has been determined that several vials of Mycobacterium Tuberculosis, Smallpox and HIV were missing.
Luckily Dr. Donleys injuries were not life threatening; however, she did seem to have a concussion and couldnt
identify the attacker(s). The FBI and a special Monarch homeland security team have been called in to investigate.

FBI Report

Blood underneath Dr. Donleys fingernails: O+ and A-

Lip prints on 2 glasses matching: Dr. Donley, Dr. Grapevine and Dr. Doctor

Fingerprints on Dr. Donleys glass matching Dr. Donley and Dr. Doctor

The stolen pathogens were: TBH, HIV, and Smallpox

An insurance document was found with blood on it of type: O+ (Dr. Donley)

An attempt to poison Dr. Doodles Donley by cyanide was found in her cocktail glass. Luckily the glass was tipped over
before Dr. Donley could partake.

Dr. Donleys time of attack is estimated to be between midnight and 2 am.

Suspects
Sarah

Austin Newman Powers

Blood Type A-

Part of an exclusive underground cult dedicated to the overthrow of Monarch University.

He has been heard saying that with Dr. Donley out of the way, the road would be clear to execute
manifest destiny.

He has been heard saying that the US should still be a colony of Britain

Currently Dr. Powers whereabouts are unknown--is he in the U.S. or back in the U.K.

Dr. Powers was also a good friend of Dr. Islands

Interview
https://drive.google.com/file/d/0B4j5KHORTT0-cDhPMm9GUGVSUkU/view?usp=sharing

Valerie Grapevine

Blood Type O+

Dr. Grapevine was very suspicious of Dr. Donley.

Last week she was caught rummaging through Dr. Donleys athletic bag looking for something.
Know one knows what, but the rumors are flourishing in the walls of Monarch University.

Apparently, Dr. Grapevine was the only one to know that Dr. Doodles had a twin sister

Latricia Doctor

Blood Type A+

Dr. Doctor has a Ph.D. in theatrical arts.

She was reportedly furious that Dr. Donley refused to acknowledge her as a colleague.

Dr. Donley was so egotistical that she often told Dr. Doctor that her Ph.D. wasnt in a legitimate
content area. Dr. Doctor vowed to set Dr. Donley straight about her ignorance!

Dr. Doctor was a loved professor by her students and she desperately wanted the grant that was
awarded to the science department.

Interview
https://drive.google.com/file/d/0B-qGGNL5fHbic19uZUNfYXA5akU/view?usp=sharing

Kelvin High

Blood Type B+

Dr. High was jealous of Dr. Donleys popularity and knowledge of string and M theory.

He felt he would never live up to Dr. Brian Greene in the eyes of Dr. Donley. If only he could
discover the gravitonthen gravity wouldnt get him down, and he would impress Dr. Donley and be
on top of the world!

Dr. Doodles reported that Dr. High had hit on her sister at the party, but she turned him down as she
has always had her heart set on Dr. Stretch, an unrequited love.

Lazarus Dangerkitty

Blood Type B+

Dr. Dangerkitty works in the lab above Dr. Donleys.

He has always been jealous of Dr. Donleys ability to acquire grant monies.

He has been overheard saying that if he could only dissect Dr. Donleys brilliant mind, he could
acquire the information to rule the world with his plastic cats.

Be careful he is known to have multiple personalities and a cunning mind!

Dr. Dangerkitty also has a vast knowledge of poisons as he has always had a hobby of reading about
the kings of old and how they were poisoned

Crime Scene
Sarah

Station 1- short brown and white hair on coat, fingerprint found in

incubator matching suspect 3
Vial with white powder with letter EXH D, petri dish in incubator A, B, C, D

Station 2- Lipstick stains on 2 glasses/ 1 napkin napkin with spilled drink on

it, blood over scene matching A-, fingerprints on glass matching Dr. Donley
and Dr. Doctor

Station 3- article ripped out of newspaper, blood on insurance policy,

articles spread out over table with different topics such as bioterrorism

Station 4- Journal entries with torn corner, entry crossed out on 1/15/16 of a
name

Station 5- Body outline splattered with blood and a large shoe print next to
body

Station 6- Fingerprint analysis matching Dr. Doctor Elisa test tested

positive for suspects 2 and 3 (Grapevine and Dr. Doctor) blood analysis Acyanide found in Doodles glass

Who is to Blame?
Sarah

Doctor Latricia Doctor is the Guilty Party

DNA found at crime scene

Fingerprint matching that found on the incubator
Fingerprint found on glass filled with poison to kill Dr. Donley
Confessed in interview to having access to poison and a proper motive
Motive was that a large grant was made towards the science department over
the theatre department
Had personal vendetta against Donley, Donley said Dr. Doctor was not a real
doctor and even Stole her man
Tested positive on ELISA test (exposed to pathogen)

Science Behind it
Gigi

Science of Pathogens- Tuberculosis (TB)

Gigi

TB is caused by a bacterium called Mycobacterium tuberculosis.

The bacteria usually attacks the lungs, but TB bacteria can attack any part of the body such as the kidney, spine, and brain.
TB is spread through the air from one person to another.
TB is NOT spread by shaking someone's hand, sharing food or drink, sharing toothbrushes, or kissing.
TB symptoms

A bad cough that lasts 3 weeks or longer

Pain in the chest

Coughing up blood

Weight loss

No appetite

Chills

Fever

Sweating at night
There are two types of tests that are used to test for TB, the skin test and the blood test.
Not everyone infected with TB bacteria becomes sick. As a result, two TB-related conditions exist: latent TB infection and TB
disease.

Latent TB infection- the TB bacteria can live in the body without making you sick.

TB disease- TB bacteria become active if the immune system cant stop them from growing.
Treatment- TB disease can be treated by liking several drugs for 6 to 9 months. Latent TB is treated by a drug so TB disease
does not develop.
If not treated properly, TB disease can be fatal.

Science of Pathogens- Human Immunodeficiency

Virus (HIV)
Gigi

HIV attacks the bodys immune system, specifically the CD4 cells, which help
the immune system fight off infections.
If left untreated, HIV reduces the number of CD4 cells in the body, making the person more likely to get infections or
infection-related cancers.
Over time, HIV can destroy so many of these cells that the body cant fight off infections and disease.
No effective cure for HIV currently exists, but with proper treatment and medical care, HIV can be controlled.
HIV Symptoms

Fever

Chills

Rash

Night sweats

Muscles aches

Sore throat
More than 1.2 million people in the United States are living with HIV infection, and almost 1 in 8 (12.8%) are unaware of
their infection.
Gay, bisexual, and other men who have sex with men, particularly young black/African American men, are most
seriously affected by HIV.

Science of Pathogens- Smallpox

Gigi

Smallpox is a serious, contagious, and sometimes fatal infectious disease,

There are two clinical forms of smallpox.

Variola major- is the severe and most common form of smallpox, with a more extensive rash
and higher fever.

Variola minor- is a less common presentation of smallpox, and a much less severe disease, with
death rates historically of 1% or less.
There are four types of variola major smallpox: ordinary, modified, flat, and hemorrhagic.

There is no proven treatment for smallpox disease. Prevention is achieved

through vaccination.
Smallpox can be prevented by the smallpox vaccine. If given to a person
before exposure to the virus, the vaccine can protect them.
Smallpox is caused by the variola virus that emerged in human populations
thousands of years ago.

Science of DNA Fingerprinting

Sarah

DNA Fingerprinting is predominantly used to compare biological evidence to a suspect in a criminal investigation
The way to confirm the DNA Fingerprint is by comparing the markings to that of the DNA at the crime scene and see
which lines line up, needs to be a fairly identical fingerprint to match
DNA fingerprinting was used in this lab in order to match the DNA that was found at the crime scene with that of one
of the 5 suspects (Matched with Dr. Doctor)
DNA Fingerprinting can also be used to determine parentage but it is most commonly used in crime scene
The procedure used to obtain the DNA Fingerprint is as follows

Obtain cells containing DNA, extract the DNA and purify

Using enzymes, the DNA is cut along certain points in the chain

The DNA is then loaded into a gel and then sending an electric current through that gel

The shorter the fragment of DNA the faster it moves towards the positive end of the current
DNA Fingerprinting does not require a lot of DNA to function- it requires as little as 1 drop of blood which makes it
ideal for crime scenes were high amounts of DNA may not be present
DNA Fingerprinting has also been used to identify organisms that cause diseases

DNA Fingerprint Graph

Our DNA Fingerprint Test matched to Dr. Doctor

Science of Hair, Fingerprints, Blood Tests, ELISA

Tests Gigi

Hair- In humans, hairs found on the head, pubic region, arms, legs, and other body areas have characteristics that can determine their origin.
Because hairs can be transferred during physical contact, their presence can associate a suspect to a victim or a suspect/victim to a crime
scene. The types of hair recovered and the condition and number of hairs found all impact on their value as evidence in a criminal
investigation.
Fingerprints- Fingerprints identified at crime scenes fall into three categories:

Patent

Latent

Impressed

Patent fingerprints are those that are clearly visible to the naked eye and are normally made because the individual has had
their fingers in some sort of liquid or powder.

Latent fingerprints are prints that are not visible to the naked eye but are visible under certain conditions; they can be made
visible by introducing them to powder or chemical agent.

Impressed fingerprints are those that have been made in soft material or tissue by pressing down with the finger or hand.
Blood tests- Blood testing is important in crime scene investigation because it is another way to identify the victim or suspect. Once clean
blood can be hard to see unless a UV light is being used.
ELISA test- An enzyme-linked immunosorbent assay, also called ELISA or EIA, is a test that detects and measures antibodies in your blood.
This test can be used to determine if you have antibodies related to certain infectious conditions. Antibodies are proteins that your body
produces in response to harmful substances called antigens. An ELISA test may be used to diagnose:

HIV

Lyme disease

Syphilis

Toxoplasmosis

rotavirus

Bibliography
"Basic TB Facts." Centers for Disease Control and Prevention. Centers for Disease Control and Prevention, 13 Mar. 2012. Web. 19 Apr.
2016. <http://www.cdc.gov/tb/topic/basics/default.htm>
"Blood." Forensic Science -. N.p., n.d. Web. 19 Apr. 2016. <http://www.forensicscience.ie/Services/Forensic-Areas/Biology/Blood/>
"ELISA." Healthline. N.p., n.d. Web. 19 Apr. 2016. <http://www.healthline.com/health/elisa#Overview1>
FBI. FBI, 01 Mar. 2011. Web. 19 Apr. 2016. <https://www.fbi.gov/about-us/lab/forensic-science-communications/fsc/july2000/deedric1.htm>
"Smallpox Disease Overview." CDC Smallpox. N.p., n.d. Web. 19 Apr. 2016. <http://www.bt.cdc.gov/agent/smallpox/overview/disease-facts.
asp>
"U.S. Statistics." U.S. Statistics. N.p., n.d. Web. 19 Apr. 2016. <https://www.aids.gov/hiv-aids-basics/hiv-aids-101/statistics/>
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Bibliography cont.
"DNA Forensics." - GeneEd. N.p., n.d. Web. 19 Apr. 2016.
<https://geneed.nlm.nih.gov/topic_subtopic.php?tid=37&sid=38>
The Editors of Encyclopdia Britannica. "DNA Fingerprinting." Encyclopedia Britannica Online. Encyclopedia Britannica, n.d. Web. 19 Apr. 2016.
<http://www.britannica.com/science/DNA-fingerprinting>
"DNA Profiling." Wikipedia. Wikimedia Foundation, n.d. Web. 19 Apr. 2016.
<https://en.wikipedia.org/wiki/DNA_profiling>