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Identifikasi tumbuhan
(Lanjutan)
(Lanjutan)
Keterangan:
1.
2.
3.
4.
5.
6.
11
9
8
7
10
6
5
4
3
2
1
Keterangan :
1. Rambut penutup
2. Epidermis
3. Hypodermis
4. Periderm
5. Berkas pembuluh koleteral
6. Sklerenkim
7. Parenkim korteks
8. Sel minyak
9. Butir pati
10. Endodermis
11. Parenkim pusat silinder
Rimpang temulawak
disortasi basah
dirajang
dikeringkan di lemari pengering
disortasi kering
diserbuk
Serbuk simplisia rimpang temulawak
Karakterisasi simplisia
- Pemeriksaan makroskopik
di saring
- Pemeriksaan mikroskopik
maserat
- Pk air
dipekatkan dengan
di freeze dryer
Ekstrak
Pengujian farmakologi
Tikus
Dipuasakan selama 24 jam
Dibunuh
Preparasi organ
Tahapan pengujian
Tiap hewan dibagi 3 perlakuan
Dengan pemberian
asetilkolin konsentrasi
bertingkat
Dengan pemberian
ekstrak temulawak
konsentrasi berturut
Pengujian Farmakologi
acetylkolin 2
BOTTOM
2.288
2.262
TOP
3.051
2.754
LOGEC50
-4
-3.944
EC50
0.0001001
0.0001138
Hill Slope
BOTTOM
0.02591
0.02655
TOP
0.04269
0.04516
LOGEC50
0.1315
0.212
Hill Slope
BOTTOM
2.23 to 2.346
2.203 to 2.322
TOP
2.956 to 3.146
2.653 to 2.855
LOGEC50
-4.293 to -3.707
-4.416 to -3.471
EC50
5.098e-005 to 0.0001965
3.836e-005 to 0.0003378
Hill Slope
1 to 1
1 to 1
Degrees of Freedom
10
10
R Squared
0.966264
0.916265
0.04214
0.04502
Sy.x
0.06492
0.0671
13
13
Best-fit values
Std. Error
Goodness of Fit
Data
Analyzed
Dose
Channel 4
Channel 4
1.00E-08
2.249
2.201
3.00E-08
2.256
2.218
1.00E-07
2.248
2.233
3.00E-07
2.265
2.255
1.00E-06
2.306
2.266
3.00E-06
2.322
2.295
1.00E-05
2.462
2.387
3.00E-05
2.522
2.466
1.00E-04
2.686
2.485
3.00E-04
2.72
2.539
1.00E-03
3.011
2.622
3.00E-03
3.031
2.763
extrak 2
BOTTOM
-1.076
-0.4576
TOP
0.5021
1.205
LOGEC50
-4.735
-4.736
EC50
1.84E-05
1.84E-05
Hill Slope
-3.184
-3.067
BOTTOM
0.01208
0.01025
TOP
0.06529
0.05655
LOGEC50
0.0169
0.01406
Hill Slope
0.2745
0.2072
BOTTOM
-1.105 to -1.046
-0.4827 to -0.4326
TOP
0.3423 to 0.6618
1.066 to 1.343
LOGEC50
-4.777 to -4.694
-4.77 to -4.702
EC50
1.673e-005 to 2.024e-005
1.697e-005 to 1.988e-005
Hill Slope
-3.856 to -2.512
-3.574 to -2.56
Degrees of Freedom
R Squared
0.998529
0.999088
0.002636
0.001774
Sy.x
0.02096
0.0172
10
10
Best-fit values
Std. Error
Goodness of Fit
Data
Analyzed
Dose
extrak 1
extrak 2
Channel 2
Channel 3
0.00E+00
3.046
4.45
1.00E-05
0.3025
0.9817
2.00E-05
-0.3809
0.268
3.00E-05
-0.8299
-0.1698
4.00E-05
-0.9392
-0.2874
5.00E-05
-1.008
-0.3899
6.00E-05
-1.015
-0.4299
7.00E-05
-1.047
-0.442
8.00E-05
-1.057
-0.4441
9.00E-05
-1.073
-0.4304
1.00E-03
-1.103
-0.4535
ATROPIN 3
Best-fit values
BOTTOM
0.2243
0.6168
TOP
-3.015
-1.778
LOGEC50
-6.775
-7.262
1.68E-07
5.46E-08
BOTTOM
0.2543
0.1764
TOP
0.1501
0.05386
LOGEC50
0.1518
0.1062
Hill Slope
EC50
Hill Slope
Std. Error
-0.4293 to 0.878
0.1634 to 1.07
TOP
-3.401 to -2.63
-1.916 to -1.639
LOGEC50
-7.165 to -6.384
-7.535 to -6.989
EC50
6.843e-008 to 4.126e-007
2.914e-008 to 1.025e-007
Hill Slope
1 to 1
1 to 1
Goodness of Fit
Degrees of Freedom
0.972384
0.98807
0.3081
0.05154
Sy.x
0.2482
0.1015
R Squared
Data
Analyzed
ATROPIN ATROPIN
1
3
Dose
Channel 1
Channel 3
-1.013
1.439
1.00E-08
0.3153
0.3172
3.00E-08
-0.6284
-0.3741
1.00E-07
-0.9411
-0.8787
3.00E-07
-1.887
-1.316
1.00E-06
-2.322
-1.688
3.00E-06
-2.785
-1.679
1.00E-05
-2.957
-1.757
3.00E-05
-3.208
-1.876
Gambar 8. Power la
(Lanjutan)
a.
Berat sampel
= 5,000g
Volume air
= 0,4 ml
Kadar air
Berat sampel
= 5,008 g
Volume air
= 0,4 ml
Kadar air
Berat sampel
= 5,012 g
Volume air
= 0,4 ml
Kadar air
b.
c.
(Lanjutan)
d.
Berat sampel
= 5,006 g
Berat sari
= 0,2784 g
Kadar sari
Berat sampel
= 5,001 g
Berat sari
= 0,2563 ga
Kadar sari
Berat sampel
= 5,001 g
Berat sari
= 0,2796 g
Kadar sari
e.
f.
(Lanjutan)
g.
Berat sampel
= 5,008 g
Berat sari
= 0,2149 g
Kadar sari
Berat sampel
= 5,007 g
Berat sari
= 0,2130 g
Kadar sari
Berat sampel
= 5,005 g
Berat sari
= 0, 2017 g
Kadar sari
h.
i.
4. Hasil penetapan kadar abu total dan kadar abu tidak larut dalam
asam