Enzyme volume (l)

0
3
6
9
12
15
18

Rate of absorbance (min-1)

0.000
0.025
0.045
0.065
0.075
0.105
0.120

Enzyme volume (l)

12
12
12
12
12
12

Rate of absorbance (min-1)

0.035
0.040
0.045
0.045
0.040
0.035

Enzyme activity (U)

0.000
0.005
0.009
0.013
0.015
0.021
0.024

Substrate concentratio Enzyme Activity without inhibiEnzyme Activity with inhibitor (U)
0.000
0.000
0.0000
0.025
0.005
0.0006
0.050
0.008
0.0010
0.10
0.013
0.0032
0.15
0.014
0.0054
0.20
0.016
0.0066
0.25
0.017
0.0074
0.30
0.019
0.0080
1/[S]

1/V

1/V inhibited

40.0
20.0
10.0
6.7
5.0
4.0
3.3

200.0
125.0
76.9
71.4
62.5
58.8
52.6

1800.0
f(x) = 44.3900556178x - 53.53040164

1600.0

1400.0

1200.0

1000.0

1666.7
1000.0
312.5
185.2
151.5
135.1
125.0

1400.0

1200.0

1000.0
1/V
800.0

600.0

400.0

200.0

f(x) = 3.9799756659x + 41.8698460639

-30 .0

-2 0 . 0

-1 0 . 0

0.0

0.0
10.0
1/[S]

20.0

30.0

40.0

50.0

y with inhibitor (U)

0.030
0.025
0.020

Enzyme activity (moles.min-1)

0.015
0.010
0.005
0.000
0

Enzyme

0.0

50.0

in-1)

0.030
0.025
0.020
0.015
0.010
0.005
0.000
0

10

12

Enzyme volume (l)

14

16

18

20