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7. Soak several pieces of the 3MM filter paper wick in 20 x SSC and place in a clean
tray.
8. Carefully place the gel on the filter paper, avoiding trapping air bubbles under the
gel.
9. Surround 3 edges of the gel with clingfilm and if necessary, top up with a small
amount of 20 x SSC to ensure the filter paper remains damp, before adding the
last piece of clingfilm.
10. Put a small amount of 20 x SSC on the gel, and then remove the Hybond filter
from the 2 x SSC and carefully place it onto of the gel, avoiding trapping air
bubbles.
11. Place the soaked 3MM filter paper onto of the gel and if needed, top up with
another small amount of 20 x SSC to keep the filter paper damp. Gently roll over
the top with a glass pipette to ensure no air bubbles are trapped.
12. Add the paper towels on top, then cover with a sheet of glass and place a weight
on top. Leave overnight.
13. The following day, the filters should be exposed to UV light to bind the DNA to the
filter. They are then ready to be hybridised and probed before being exposed to xray film.