Mechanisms in Hematology: B Lymphocytes Antigen-Independent Differentiation In The se eee Genes | cytokines marke Bone Marrow The sites of B lymphocyte generation vary with the 3 ee | ope different developmental stages. During embryonic ©) a io development, the first B cell precursors are detected} | 3 i coca in the omentum and the liver. After birth, the bone 33 ° a marrow takes over as the major site of B cell Jl e development, which begins near the endosteum 3a v and moves toward the centre of the marrow cavity Fd e ves as the cell differentiates. The hallmark of this stage voy of B cell differentiation is rearrangement and expression of the immunoglobulin genes; in 7 O™ vosuK ly addition, there are changes in expression of cell “a” u Le surface and intracellular proteins. 9 Be Worn] | ht Development of mature B cells from HSCs depends | | |o| on contact with stromal cells; different stromal cells | |< 6 wom] oS, are involved at various stages of differentiation. je a T Stroma (Greek: mattress) is a generic term that . nm includes a variety of cells such as fibroblasts, 5 oO endothelial cells, macrophages, and adipocytes, 5 T Stromal cells regulate 8 cell development in a 03 number of ways: (1) by direct interaction, (2) by e@ @ formation of the extracellular matrix (collagen, Bs eS a 0 proteoglycans) which regulates cell migration and e binds growth factors, and (3) by release of i v Stam cytokines. tose