Académique Documents
Professionnel Documents
Culture Documents
Under Supervision of :
Submitted by :
Nitish kumar
Department of Biotechnology
14001509009
CONTENTS
INTRODUCTION
OBJECTIVES
REVIEW OF LITERATURE
MATERIALS AND METHODS
i) CHARACTERIZATION
ii) ANTIBACTERIAL ACTIVITY
iii) FREE RADICAL SCAVENGING ACTIVITY
SIGNIFICANCE OF STUDY
REFERENCES
INTRODUCTION
It offers better built, longer lasting, cleaner, safer and smarter products for the
home, for communications, for medicine, for transportation, for agriculture, and
for industry in general.
Silve nanoparticles are of great interest for use as antimicrobial agents. Smaller
Silver Nanoparticles have greater antibacterial activity hence they are new
generation of antimicrobials
Calotropis is used alone and sometimes with other plants to cure variety
of human and animals ailments. It is used in various polyherbal
preparations. Different parts of the plant have immense potential to cure
various diseases and disorders.
Skin diseases
Antiviral activity
Anti-inflammatory activity
Analgesic and Antinociceptive
Activities
Activity
Arrow poison
PartsAnti
Usedtumor activity
Latex
Biocidal activity
Latex
Cleansing water
Energy plantation
Leather tanning
Fungicidal, insecticidal
Whole Plant
Whole Plant
Whole Plant
OBJECTIVES
activity
nanoparticles.
of
synthesized
silver
REVIEW OF LITERATURE
Many studies have been conducted on, silver nanoparticles using different
parts of plants like nodal, leaves and whole plant by various researchers
S.No
1
green
synthesis
of
Outcomes
and Face centered cubic (FCC) structure and
silver size was ranging from 19 to 45 nm (L) and
nanoparticles
Calotropis
concentration
and
the
complete
S.No
Work done
Outcomes
of
Silver nanoparticles
Nanoparticles
Using Calotropis gigantean
Leaf
was
observed
nanoparticles
Ocimum sanctum.
limon
S.No
G. Geoprincy et
al, 2012
Work done
Outcomes
diffraction
and
Fourier
SYNTHESIS OF SILVER
NANOPARTICLES
Collect fresh leaves
Wash with distilled water to remove dust (3 times).
Finely chopped + 100ml D.W
Boil for 15 min. and then cool (at 60 C ) .
Filter using whatmann filter paper.
Freeze dry the extract (-70C2C; 48 h)
Store at 4C
Continued
CONTINUED
CHARACTERIZATION
Characterization
refers to the study of materials
features such as its composition, structure, & various
properties like physical, electrical, magnetic etc.
UV visible spectroscopy: In general 200-800 nm light wavelength
is used for the characterization of size range 2-100 nm.
X-Ray Diffraction (to study structure, crystallinity, particle size,
distribution, particle shape and homogeneity of whole sample)
ANTIBACTERIAL ACTIVITY
AgNPs
AgNO3
REFERENCES
Gamble J S; Flora of the Presidency of Madras, Vol. I, II, III, Botanical survey of India,
Calcutta, 1935.
Saikia D, Gogoi PK, Phukan P, Bhuyan N, Borchetia S, Saikia J. Green synthesis of silver
nanoparticles using Asiatic Pennywort and Bryophyllum leaves extract and their antimicrobial
activity. Adv. Mater. Lett. 2015; 6: 260-264.
manjul gondwal and geeta joshi nee pant* biological evaluation and green synthesis of silver
nanoparticles using aqueous extract of calotropis procera Int J Pharm Bio Sci 2013 Oct; 4(4):
(P) 635 643
Baskaralingam Vaseeharan,1 Clara Gunapoorani Sargunar,2 Yong Chin Lin,3 Jiann Chu Chen3
Green synthesis of Silver nanoparticles through Calotropis gigantea leaf extracts and
evaluation of antibacterial activity against Vibrio alginolyticus Italy Nanotechnology
Development 2012; 2:e3 doi:10.4081/nd.2012.e3
Singhal G, Bhavesh R, Kasariya K, Sharma AR, Singh RP. Biosynthesis of silver nanoparticles
using Ocimum sanctum (Tulsi) leaf extract and screening its antimicrobial activity. J Nanopart
Res. 2011; 13: 2981 2988.
Farooqui MA, Chauhan PS, Krishnamoorthy P, Shaik J. Extraction of silver nanoparticles from
the leaf extracts of Clerodendrum inerme. Dig J Nanomater Bios. 2010; 5: 4349.
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