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The endospore stain is a differential stain used to visualize bacterial endospores.

Endospores are formed by a few genera of bacteria, such as ©
. By forming spores,
bacteria can survive in hostile conditions. Spores are resistant to heat, dessication, chemicals,
and radiation. Bacteria can form endospores in approximately 6 to 8 hours after being exposed to
adverse conditions. The normally-growing cell that forms the endospore is called a vegetative
cell. Spores are metabolically inactive and dehydrated. They can remain viable for thousands of
years. When spores are exposed to favorable conditions, they can germinate into a vegetative
cell within 90 minutes.

Endospores can form within different areas of the vegetative cell. They can be central,
subterminal, or terminal. Central endospores are located within the middle of the vegetative
cell. Terminal endospores are located at the end of the vegetative cell. Subterminal endospores
are located between the middle and the end of the cell.

Endospores can also be larger or smaller in diameter than the vegetative cell. Those that are
larger in diameter will produce an area of "swelling" in the vegetative cell. These endospore
characteristics are consistent within the spore-forming species and can be used to identify the
organism.

Because of their tough protein coats made of keratin, spores are highly resistant to normal
staining procedures. The primary stain in the endospore stain procedure, malachite green, is
driven into the cells with heat. Since malachite green is water-soluble and does not adhere well
to the cell, and since the vegetative cells have been disrupted by heat, the malachite green rinses
easily from the vegetative cells, allowing them to readily take up the counterstain.

The staining procedure is here.

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 What is the function of the endospore to bacterial cells?
Asked by The Goddess - Wed Sep 20 20:44:28 2006 - - 2 Answers - 0 Comments

 Bacteria form endospores when they have some sort of stress. The endospores are pretty
resistant to all sorts of things that would normally kill the bacteria (such as heat or low food). It's
the bacteria's way of making sure its genetic material survives through the tough times.
Answered by bjfrancois5 - Wed Sep 20 21:26:40 2006

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 from that endospore. True or False?
Asked by Cat - Sun Sep 14 14:46:25 2008 - - 1 Answers - 0 Comments

 True, it's the same cell... the only difference I can think of would be in the number of some
organelles and cytoplasm which it would have to regenerate
Answered by ladybug - Tue Sep 16 15:01:12 2008
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 If a bacterium regenerates from an endospore that did not possess any of the plasmids that
were contained in its original parent cell, the regenerated bacterium will probably a. lack
antibiotic-resistant genes. b. be unable to survive in its normal environment. c. lose base pairs
from its chromosome. d. lack a cell wall. e. lack a chromosome.
Asked by dreamxxfly - Mon Jun 14 21:35:12 2010 - - 1 Answers - 0 Comments

 A. plasmids are genes that are found outside the bacteria's main chromosome(s). the
chromosome contains all the genetic information needed for the bacteria to function, B is wrong.
it WILL be able to survive in its normal environment (unless that environment happens to be
filled with antibiotics. C. i dont know why it would lose base pairs... plasmids are not part of the
chromosome D. cell wall would be part of the genetic code found in chromosomes E. it isnt
missing a chromosome...its missing a plasmid so your correct answer is A. plasmids tend to carry
antibiotic resistant genes. bacteria can then swap plasmids with each other, increasing their
resistance to antibiotics. something like "bacteria sex" =P.
Answered by DT - Mon Jun 14 21:46:24 2010

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 What is the difference between endospore and exospore?
Asked by sami - Sat Jun 3 02:45:42 2006 - - 10 Answers - 0 Comments

 An endospore is any spore that is produced within an organism usually a bacterium This is in
contrast to exospore s which are rather produced by growth or budding The primary function of
most endospores is to ensure the survival of a colony. st
Answered by Starreply - Sat Jun 3 02:55:33 2006

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 what is an endospore and how does it help the tetanus survive? I really need help on this :)
Thankyou :)
Asked by stephanie R - Wed Apr 2 11:15:17 2008 - - 1 Answers - 0 Comments

 The endospore is a dormant, tough, and non-reproductive structure produced by bacteria. It is

highly environmentally resistant, especially to heat and antiseptics. It helps the bacterium survive
adverse conditions.
Answered by OKIM IM - Wed Apr 2 11:22:55 2008

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c

 
 I'm doing a project, and i'm trying to search on websites if my bacteria has an endospore
formation. anyone help? this bacteria creates typhoid fever!
Asked by Retro Metro - Mon Feb 8 20:29:18 2010 - - 1 Answers - 0 Comments

 Salmonella Sp do not form endospores

Answered by Merlin's Feline - Mon Feb 8 20:57:10 2010

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 How would an endospore stain of Mycobacterium appear?
Asked by wiccanwitch_24 - Tue Oct 16 02:29:55 2007 - - 2 Answers - 0 Comments

 The mycobacteria are rod-shaped bacteria generally 0.4 by 3.0 uem in size that are said to be
acid-fast. This means that because of their unique cell wall, when they are stained by the acid-
fast procedure, they will resist decolorization with acid-alcohol and stain red, the color of the
initial stain, carbol fuchsin. With the exception of a very few other acid-fast bacteria such as
Nocardia, all other bacteria will be decolorized and stain blue (the color of the counterstain,
methylene blue). The acid-fast stain is an important test for the genus Mycobacterium.
Fluorescent microscopy staining may also be used to identify Mycobacterium.
Answered by Bethyll D - Tue Oct 16 02:34:43 2007

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 What advantage to a bacterium would an endospore be?
Asked by bchbum2007 - Mon Sep 21 18:12:33 2009 - - 1 Answers - 0 Comments

 The bacterium could survive as an endospore in a dry environment until such time as a
moisture film returned so the bacteria could actively live.
Answered by emucompboy - Mon Sep 21 19:21:48 2009

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 what are conditions are favorable for endospore germination? Every resource I find just states
"favorable conditions" and doesn't explain what those conditions are...
Asked by jazzy - Wed Jul 22 15:52:57 2009 - - 1 Answers - 0 Comments

 Maturity of cells, depletion of nutrients, accumulation of wastes all stimulate production of

spores.
Answered by saffronesque - Wed Jul 22 16:15:11 2009

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 What are the major steps involved in endospore formation?
Asked by bacteria - Sun Feb 25 00:23:30 2007 - - 1 Answers - 0 Comments

 Endospores and Endospore Staining --- Endospore Formation Endospores are formed by
vegetative cells in a process called sporulation. Sporulation is initiated when conditions for
growth of the vegetative cells become harsh (for example, when water or an essential nutrient is
limiting). Sporulation is a complex process involving as many as 200 genes. These genes are
activated by an environmental trigger, causing sporulation of the cell. The steps leading to
endospore formation result in the creation of a dry, metabolically inert and extremely resistant
endospore from a moist, metabolically active vegetative cell. Extensive sporulation studies have
been done on Bacillus subtilis, and this microbe can perform the entire« [cont.]
Answered by dcny21 - Sun Feb 25 00:28:40 2007

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 What is the function of a bacterial endospore?
Asked by adebolaji22 - Mon Oct 30 14:53:47 2006 - - 2 Answers - 0 Comments

 when essential nutrients are depleted, ceratin gram positive bacteria form these
endospores.They are very duable and can with stand extreme heat, cold, lack of water, exposure
to mony toxic chemicals and still survive. Thier essential function is to carry on the bacteria's
life, they ensure the survival of a bacterium through periods of environmental stress. and can live
for millions of years!
Answered by tiff-so-fierce - Mon Oct 30 15:41:55 2006

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 Thanks!
Asked by Stephanie and Renae D - Mon May 19 23:50:36 2008 - - 1 Answers - 0 Comments

 Yes, specimens need to be heat-fixed on the slide so that the material will not fall off during
staining.
Answered by OKIM IM - Tue May 20 00:20:03 2008

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 why is it not necessary to include a negative control for endospore staining?
Asked by Just Breathe - Sat Mar 21 20:45:15 2009 - - 1 Answers - 0 Comments

 When you stain endospores no control is needed because endospores are all identical (in terms
of properties.) Thus, control groups are not needed.
Answered by Mikkie - Sat Mar 21 21:48:15 2009
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 Of what advantage to clostridium is an endospore?
Asked by prettyamericanangel - Fri Feb 10 02:55:48 2006 - - 2 Answers - 0 Comments

 The same advantage it is to any bacterium. It's a thick walled cell that allows the bacterium to
remain dormant in times of stress without dying.
Answered by kunwarrarra - Fri Feb 10 03:37:04 2006

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 A) Carry out fermentative metabolism B) Don't produce endospores C) Lack cell walls D)
Are nonpathogenic E) Are aerotolerant
Asked by unknown - Wed Nov 4 13:33:00 2009 - - 1 Answers - 0 Comments

 A) Carry out fermentative metabolism

Answered by Alone GuY - Sat Nov 7 18:38:09 2009

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 #
 
 
 In the endospore stain of Bacillus megaterium I noticed the endospores, but could not tell if
the spores in the middle of the vegetative cell or at a terminal end. I also couldn't tell if the
endospore was swollen where it appearred to be pushing on the wall of the vegetative cell or if it
just looked like a circle inside the cell with no swelling. Can someone help?
Asked by Amanda F - Wed Sep 23 18:37:24 2009 - - 1 Answers - 0 Comments

 when you do these labs you need to make drawings and ask the instructor if something seems
ambiguous to you. No one can see into your microscope right now.
Answered by unknown - Wed Sep 23 18:43:57 2009

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 How does an endospore form and what does it do?
Asked by unknown - Sun Apr 4 18:04:51 2010 - - 1 Answers - 0 Comments

 Formation: When a bacterium detects environmental conditions are becoming unfavorable it

may start the process of sporulation, which takes about eight hours. The DNA is replicated and a
membrane wall known as a spore septum begins to form between it and the rest of the cell. The
plasma membrane of the cell surrounds this wall and pinches off to leave a double membrane
around the DNA, and the developing structure is now known as a forespore. Calcium
dipicolinate is incorporated into the forespore during this time. Next the peptidoglycan cortex
forms between the two layers and the bacterium adds a spore coat to the outside of the forespore.
Sporulation is now complete, and the mature endospore will be released when the surrounding«
[cont.]
Answered by gootiween - Sun Apr 4 18:09:27 2010

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 Help!! BIO!! please!
Asked by Rachael W - Wed Nov 21 18:49:34 2007 - - 1 Answers - 0 Comments

 so the stuff inside has a way out !

Answered by Rich P - Wed Nov 21 19:01:46 2007

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$$
 help!
Asked by twiztedweb - Thu Oct 18 02:11:46 2007 - - 1 Answers - 0 Comments

 It doesnt have any, so it is negative in an endospore stain.

Answered by QuiteNewHere - Thu Oct 18 02:20:58 2007

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 Are acid-fast stains AND endospore stains heat fixed prior to heating (steaming) them for 5
minutes?
Asked by jon & Corrie A - Sun Feb 25 16:53:48 2007 - - 2 Answers - 1 Comments

 You use the small paper towel with the green malachite to keep the endospore intact and
moist. On the acid fast my book says that you need to fix your smear on the slide with one of two
methods, run the slide through the blue flame 2-3 times, or leave the slide with the smear in a
slide warmer @ 60 degrees for 10 minutes.
Answered by kam - Sun Feb 25 17:04:40 2007

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