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Vesicular trafficking
And Endomembranes

Green – vacuole membrane

Red - chloroplasts Bruce D. Kohorn
Bowdoin College

Figure 12-1

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Figure 12-2

Subcellular fractionation
Differential centrifugation

Figure 12A-1

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Density gradient centrifugation

Figure 12A-5

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Rough ER

Protein synthesis and

cotranslational insertion

Protein maturation
gylycosylation
S-S bond formation
assembly of multimers

Removal of misfolded proteins –

www.DennisKunkel.com targeting for degradation
in proteasomes

Smooth ER

• Lipid metabolism and membrane biosynthesis

• Steroid biosynthesis
• Carbohydrate metabolism
• Calcium storage
• Drug detoxification

Figure 12-3

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Golgi

Biologyrefernce.com

Figure 12-4

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Figure 12-5

Lysosomes

Contain digestive enzymes

(acid hydrolases etc)

Membrane on the inside

is heavily glycosylated

pH 4-5 inside

FUNCTIONS:
Defence and nutrition
Autophagy
Extracellular digestion

Figure 12-20b

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Peroxisomes

• Contain H2O2

• Detoxification
• Hydrogen peroxide metabolism
• Oxidation of fatty acids
• Metabolism of nitrogen-containing compounds

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Radioactively labeled protein trafficking

in guinea pig’s pancreatic secretory cells.

Figure 12-10

Endocytosis and Exocytosis

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Exocytosis

Figure 12-12

Endocytosis

Figure 12-13

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Phagocytosis of large particles (>0.5μm)

Pinocytosis of liquid Figure 12-14

Receptor-mediated endocytosis

Figure 12-15

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Figure 12-16

Clathrin lattice

Figure 12-17

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Clathrin assembly

Figure 12-18

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Design an experiment that will allow you to follow a

protein from ER to Golgi to secretory vesicles and to
outside of the cell.

GFP-tagged protein
Retained at ER at 40 degrees
Released for transport at 30 degrees

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Randy Scheckman

http://www.scivee.tv/node/9284

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