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lanYFP Fusion Expression Vectors

R etroviral infection of cells is an effective means of


stably inserting a gene of interest into a host cell.
The pCHAC retrovirus is successful in infecting most
mammalian cells, including human. These vectors
are suitable for most challenging fluorescent protein
applications.

A llele’s pCHAC fluorescent protein fusion ex-


pression vectors use efficient and unique tech-
nologies to get the best results for our product. The
pCHAC retroviral vector is based on the Moloney
Murine Leukemia Virus (MMLV) and contains LTRs
and packaging signals. These vectors can be used
to express a protein of interest as either an N- or
C-terminal fusion to a fluorescent protein by inserting
cDNA into the MCS, or for packaging a stand-alone
control retrovirus that expresses an FP in infected
cells. Allele uses the brightest FPs, in their respective
spectrums, to date: mWasabi, mTFP1, LanRFP and
LanYFP. These vectors result in remarkably high titer, Box 1 | Product List
108 TU/ml and higher.
FP Fusion Vectors
pCHAC-lanYFP-C
ABP-PVL-YCNCS01 1µg
ABP-PVL-YCNCS10 10µg
pCHAC-lanYFP-N
ABP-PVL-YNNCS01 1µg
ABP-PVL-YNNCS10 10µg

lanYFP Properties
Characteristic lanYFP
Fluorescence Color Yellow
Excitation Max (nm) 513
Emission Max (nm) 524

S afety issues
Retroviral vectors should be han-
dled using NIH BSL-2 safety guide-
Quantum Yield
Extinction coefficient
0.95

150,000
(M-1cm-1)
lines. For more information, please
see Biosafety in Microbiological and pKa ~3.5
Biomedical Laboratories <4th edition>
Salt insensitive (NaCl) 0-500mM
which is available on the Web sites
of the National Institutes of Health at Brightness 143
http://bmbl.od.nih.gov.

F or Research Use Only. Not for Diagnostic or Therapeutic Use. Purchase does not include or carry any right to resell or transfer
this product either as a stand-alone product or as a component of another product. Any use of this product other than the permitted
use without the express written authorization of Allele Biotech is strictly prohibited

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