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The Ten Steps of Glycolysis


Energy Investment Phase Energy Payoff Phase
CH 2OH (in the second phase, there are 2 of each molecule)
O H
enzyme: H H H
C O
HO OH H OH
CHOH 6 2 Pi

triose phosphate
dehydrogenase
ATP H OH
CH 2 __ O __
hexokinase

P
Glucose
1 (2) Glyceraldehyde 3-phosphate
(G3P)
ADP 2 NAD+
P
__ O __ C O
CH 2O __ P 2 NADH + 2 H+
O CHOH
H H H
CH 2 __ O __

phosphoglycerokinase
P
HO OH H OH
(2) 1, 3-Bisphosphoglycerate
2 ADP
phosphoglucoisomerase

H OH
Glucose 6-phosphate O– 7
2 C O
2 ATP
CHOH
CH 2O __ P
CH 2 __ O __

phosphoglyceromutase
O P
CH 2OH
(2) 3-Phosphoglycerate
H H HO OH
O– 8
phosphofructokinase

ATP HO H
Fructose 6-phosphate C O
3 H __ C __ O __ P

ADP CH 2OH
P __ O __ CH CH 2 __ O __ P
2
O (2) 2-Phosphoglycerate
enolase

H H HO OH O– 9
HO H C O
aldolase

aldolase

Fructose 2 H2O
1, 6-bisphosphate C __ O __ P

4
CH 2
pyruvate kinase

(2) Phosphoenolpyruvate
2 ADP
__ O __ CH H
P
2 isomerase O– 10
C O
C O 5 C O 2 ATP
CHOH
C O
CH 2OH __ O __
CH 2 P

Dihydroxyacetone Glyceraldehyde CH 3
phosphate 3-phosphate (2) Pyruvate
(G3P)