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Figure 1: Oxygen

A 200 µm
C
60

Pressure (mmHg)
Partial Oxygen
40

B 20

0
0 0.2 0.4 0.6 0.8 1
Normalized Radius

5 Live Dead Apoptotic 0 0 0 0.5 0.5 1 Inner Outer Normalized Radius .A 200 µm B C E 200 µm F G H 1 1 Average Normalized Normalized Intensity Necrotic Apoptotic Intensity Live 0.

5 0.5 1 Inner Outer Normalized Radius .A 200 µm B C D E 200 µm G H 1 Necrotic Normalized Intensity Average Normalized 1 Apoptotic Live Intensity Live Dead 0.5 Apoptotic 0 0 0 0.

12 7.2 0.96 B 6.4 0.Figure 4: pH A 200 µm C 7.88 0 0.6 0.04 pH 6.8 1 Normalized Radius .

4 0.6 0.6 0.8 1 0 0.4 0.2 0.6 0.8 1 Normalized Radius Normalized Radius C Glutamine D Glutamate 40 5 (10-10 mmol/cell) (10-10 mmol/cell) Amount per cell Amount per cell 30 4 3 20 2 10 1 0 0 0 0.Figure 5: YSI A Glucose B Lactate 50 40 (10-10 mmol/cell) Amount per cell (10-10 mmol/cell) Amount per cell 40 30 30 20 20 10 10 0 0 0 0.2 0.8 1 .4 0.8 1 0 0.2 0.2 0.4 0.6 0.

64 0. Figure 6: Essential Amino Acids (10-10 mmol/cell) 60 * Valine Amount per cell Leucine 40 Iso-Leucine 20 0 0.71 0.11 Average Distance into the Spheroid (r/R) .22 0.35 0.49 0.

35 0.64 0.49 0.71 0.11 Average Distance into the Spheroid (r/R) .Figure 7: Nonessential Amino Acids Alanine 10 * Glycine (10-10 mmol/cell) Amount per cell 8 Proline Serine 6 Aspartate 4 2 0 0.22 0.

5 0. Large vs.5 0 0 Inner Outer Inner Outer Large Cylindroids Small Cylindroids (Diameter > 500 µm) (Diameter < 500 µm) . Small 1 1 Average Normalized Intensity Average Normalized Intensity Necrotic Necrotic Apoptotic Apoptotic Live Live 0.